Comparison was made among three methods of diagnosis for canine filariasis:(1) the smear and stain method, (2) the concentration method, and (3) the intradermal reaction.
Experiments were performed with 103 dogs in spring and summer and 122 dogs in the other seasons. The dogs used were all more than one year old and autopsied at the end of the experiments.
Method 1 used a thick smear of 30cmm of blood collected from the auricular vein, which was stained with methylene blue. It gave a microfilaria detection rate of 93.5 per cent in summer and 75.5 per cent in winter when applied to dogs which were found to harbor microfilariae at autopsy.
Method 2 was applied to 1cc of venous blood, using aceton. A 100 per cent detection rate of microfilariae was obtained from this method, regardless of season.
Method 3 was carried out with antigen prepared from the body of adult canine filariae which had been fractionated with acetone after the treatment with sound-waves. The antigen was dissolved in physiologic salt solution at a concentration of 0.01 per cent and injected intradermally in the hypogastric region in a net amount of 10μg/0.1cc. Positive reaction was shown in 95 per cent of those which were found parasitized at autopsy and which, nevertheless, had been negative in the detection of microfilariae from the peripheral blood.
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