In order to find out actual influences of various environmental factors upon the healthy dog, it is important to observe diurnal changes, including those in the period of pregnancy and lactation, in such factors. In this study, diurnal changes in blood constituents and serum calcium were chiefly examined. The concentration of serum ionic calcium changed a little in the course of a day, but no significant difference was recognized between the maximum and the minimum values in the measurement. The total amount of calcium in the serum had a tendency to decrease in the afternoon and increase at night. The concentration of ionic calcium and total calcium in the urine had a tendency to increase in the afternoon and decrease in the morning. The Erythrocyte count, hemoglobin and packed-cell volume rose during the daytime and fell down at night, showing no significant difference among the average values. The serum ionic calcium and total calcium of female dogs in the periods of pregnancy, delivery, and lactation had a tendency to decrease at the end of pregnancy, increase a little in the midst of delivery, and decrease again during the period of lactation. The erythrocyte count, hemoglobin and packed-cell volume had a tendency to decrease in these periods, showing no significant difference among the measured values.
Mites of Tyrophagus dimidiatus (Kenaga Kona dani in Japanese) were added to powdered milk, which was stored in sealed cans filled either with nitrogen (group Nm) or with air (group Am). Two sets of cans containing no powdered milk but mites served for control. In one set cans were filled with nitrogen (group N) and in the other with air (group A). The mites were examined for survival and reproduction inside the cans of the four groups. As a result, all mites were found dead within 12 hours in group Nm and control group N and within 48 hours in group Am. Nothing unusual was observed at all on the mites of control group A.
It was reported in the previous paper of this series that pronon, a polypropylene glycol derivative, had a remarkable effect for the prevention of bloat, as compared with several antifoaming agents. In the present study, pronon-absorbed cocoanut grounds were examined for practical effect of controling bloat. Thirty-four ewes were used made at the Iwate Livestock Breeding Station in July, 1962. Experimental animals were fed a ration containing 10g of pronon-absorbed cocoanut grounds and 100g of wheat bran per head per day. Controls were fed a ration containing 100g of wh eat bran. Then they were grazed on a Ladino-clover pasture. One of the experimental animals was not affected with bloat, but ten control animals suffered from acute bloat. Pronon prevented bloat for twenty-four hours after feeding. The surface tension and the foaming rate of rumen juices decreased significantly after pasturing. The results showed that pronon might be a marked preventive for acute foamy bloat in ruminants.