Studies were performed to clarify whether or not detection of inclusion bodies from dogs naturally infected with distemper had any value of clinical application to the diagnosis of canine distemper. Specimens were collected by curettage from the mucous membranes of the conjuctiva, nasal cavity, cheek, tongue, vagina, and prepuce of the penis, and examined for the presence of inclusion bodies. The results obtained are as follows.
1. In 74 (86.0 percent) of 86 dogs studied, cytoplasmic inclusion bodies were detected from at least one of the specimens collected from the five localities. This indicates that the detection of inclusion bodies is of great practical value in the clinical diagnosis of canine distemper.
2. The locality or localities from which inclusion bodies were detected varied greatly according to individuals.
Any significant differences were hardly observed in frequency of detection among the localities examined from a stochastic point of view. Therefore, it is recommended that specimens for detection be collected from as many localities of the body as possible.
3. No differences were present in the rate of detection of inclusion bodies among dogs which were within about 20 days after the onset of clinical symptoms of distemper.

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2, 2'-Sulfinylbis (4, 6, Dichlorophenyl) を肝蛭実験感染家兎, 肝蛭自然および人工感染緬羊, 同自然感染野外牛に投薬し駆虫力と副作用を調べ, その結果を次のように要約する.
1. 家兎肝蛭に対しては50mg/kg以上の1回, 経口投与ですべての虫体は駆虫され25mg/kgでも2頭中1頭に生存肝蛭はなく他の1頭に死減虫体7, 生存虫体2匹を検出し駆虫効力は25mg/kgにおよぶ.
2. 緬羊肝蛭に対しては20mg/kg以上の瓶投薬においてすべての肝蛭を駆虫し15mg/kgでも2頭中1頭に虫体はなく, 他の1頭には生存虫体が僅かに1匹しか検出されなかった.また, 投薬量100mg/kgまで副作用は観察されなかった.
3. 牛肝蛭に対する駆虫力は投薬方法によって著るしく異なり, 混飼投薬法は瓶による投薬法よりも高い陰転率を示した.混飼投薬法では25～30mg/kgの1回投与が適量で84.2～93.4%の陰転率をえ, この投薬量では高度の副作用はないが, 副作用発現牛は軽度かつ短期間の下痢と食欲不振であった.

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Bacterial contamination occurs very frequently during the conventional manufacturing process of tofu, or soy bean curd, a favorite food for the Japanese. Recently, tofu has come to be sold in package prepared with Hi-Zex. Since packaged tofu is heated at the final step of the manufacturing process, the sanitary quality of this food can be greatly improved.
At the end of boiling at 90°C for 40 minutes, it was found that the central part of a packed piece of tofu had been maintained at 73°C, or a safetyindicating temperature, for 20 minutes, and that 1ess than 300 1iving bacteria, including no coli-form ones, were contained in 1g of tofu in a package.
When a specimen of tofu was allowed to stand at room temperature (30°C at the highest) for 24 hours, it showed a living bacterial count of 10³, containing no coli-form organisms in it. When another specimen was allowed to stand at room temperature for 15 minutes immediately after the process of heating, it remained at a level higher than 73°C for more than 30 minutes. Accordingly, the process of heating is satisfactory from a sanitary point of view.
Packaged tofu had a heat-conducting index of 0.083. Then the temperature at the central part of a piece of tofu could be calculated from the temperature and time of heating by using the following formula: T=T∞(1-e^-kt).

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哺乳子豚10頭を4頭の母豚から選び, 分娩直後から離乳期までの哺乳期間中の血液性状の変動を調べ以下の所見を得た.
(1) 分娩直後のHb値は63.5±4.79%で1週間で44.5±4.11%, 2週間では44.75士2.73%, 4週間で41.45±2.56%, 7週間で分娩時の値にほぼ回復し63.75±1.82%を示した.
(2) RBCは, 生時457.7±9.9万でHbと同様すでに貧血の状態で娩出されている.1週間で400.8土16.5万, 2週間で483.2±38.3万, 7週間で791.95士57.6万と成豚の値に到達している.
(3) WBCは生時9, 780±2, 410と変異の幅が広く成豚に比し低い値を示している.7週間で28, 155士3, 860と著るしく増加している.
(4) 白血球像は分娩直後Nは72.3±6.28%, Lは23.4士5.96%で, この2成分がほとんど大部分を占有しており, 1週間でこの2者の比率は逆転し, Nは40.7±5.67%, Lは55.5士6.72%となる.2週間ではこの割合はさらに開く.4週間では大きな変化はないが, 7週間目では全例にEが出現し, 17.0±6, 53%を示したが, Lは50.6±9.47%と対照的に減少を示した.

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(1) 自然感染にもとづく中和抗体は, 相当の長年月の間存続する.牛によっては10年位存続することもあると考えられる.
(2) 母牛が中和抗体を有すると, 感染が不顕性感染による場合であっても, その子牛は母乳から受けついだ中和抗体を持っている.母子免疫による中和抗体は, 5ヵ月～7ヵ月位存続するようである.
(3) 活性ワクチンは, 母子免疫された子牛には, 中和抗体存続中は無効となることがあるので, その応用には注意を要する.

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