A total of 343 dogs were administered perorally with 5 and 10 mg/kg of diethylcarbamazine preparation. Of them, seven (2.0%) manifested transient salivation and vomiting and six (1.7%) fell in severe shock soon after medication on account of central nervous system involvement and died. The six dogs attacked by shock were found to have harbored Filaria larvae in the blood.(They were 4.9% of 123 dogs infected with filariasis.) They exhibited the same clinical signs as the clinical case of filariasis. From these results, it was confirmed that the administration of this preparation induced severe shock in dogs when filariae had infected them, and caused death eventually. It is presumed that the shock might result from allergy to that drug. Under these circumstances, that drug can be used for the prevention of canine filariasis only in uninfected immature dogs in which it is proved to have no larvae in the blood. When it is given per os to such dogs consecutively for a long time, its daily dose should be 11 to 22 mg/kg.
Eleven horses suffering from chronic equine infectious anemia with obscure clinical symptoms were subjected to bleeding, so that they lost such amount of blood as corresponding to 4 per cent, on the average, of body weight. They were examined for changes in hematological properties for 24 hours. The results obtained are summarized as follows. 1. The red cell count and hemoglobin value increased immediately after bleeding (a.b.), but decreased suddenly 4 hours a.b. showing a gradual decrease after that. The pigment index exhibited a gradual increase. 2. The white cell count decreased a little immediately a.b. and then increased, reaching the maximum value 8 hours a.b. After that it revealed a gradual decrease. 3. Neutrophils exhibited a shift to the left slightly and a tendency to change in number almost in parallel with the white cell count. Lymphocytes showed changes which were in reverse proportion to those of neutrophils. Eosinophils decreased in number a.b. 4. The maximum resistance of erythrocytes was reduced 4 hours a.b. The erythrocyte sedimentation rate was delayed transitorily just a.b. 5. The specific gravity and viscosity of the blood decreased immediately a.b., showing a remarkable decline 4 hours a.b. 6. There were no significant changes in the amounts of total protein, albumin, and globulin of the serum and in A/G ratio. 7. Individual differences were great and no significant differences present in the amounts of blood sugar and calcium, inorganic phosphorus, and iron of the serum, with an exception that the serum iron exhibited a significant decrease 4 hours a.b.
In the metropolitan slaughterhouse, Shibaura, Tokyo, the first case of swine toxoplasmosis (TP) was reported in December, 1959. After that, 3 cases were recorded in 1960, 12 cases in 1961, and 27 cases in 1962. Of these cases, seven were detected in the antemortem examination from among 70 pigs shipped from Niigata Prefecture in November, 1961. The other cases were found in the postmortem examination from among hogs shipped from different prefectures all over the country. The antemortem signs of TP consisted of a high temperature of over 41°C, purpura in the auricle and hypogastric region, and sometimes dyspnea. The white blood cell count was in a range of 11, 000 to 13, 000. A slight shift, to the left was seen. Intradermal toxoplasmin test was negative in all the clinical cases, which, however, gave highly positive pigment tests on serum. These results provide helpful information on the antemortem diagnosis of TP. Postmortem changes were a little more pronounced in the clinical cases than in those found infected at autopsy. They included petechiae in the apical lobe of the lung, cloudy swelling of the liver, swelling of the splenic follicles, and lymphadenopathy of these organs. Histopathologically, perivascular cell infiltration, proliferation of glia cells hemorrhagic catarrh in the pulmonary alveoli, focal necrosis of the liver, and atrophy of follicles in the lymph node were observed.