The new organic phosphorous preparation JSR-381 of low toxicity, or 0, 0-dimethy1-0-1, 2-dibromo-2, 2-dichloroethyl phosphate, was examined for anthelmintic effect on four kinds of intestinal parasites of dogs. A total of 21 dogs were employed. The standard dose applied was 75 mg per 10 pounds of body weight, or 16.7 mg/kg.
1. When the standard dose was administered daily for two consecutive days, 94.3% of the ascarids, 97.8% of hookworms, and 99.9% of whipworms were removed on the average. The mean rate of expulsion of tapeworms was only 11.0%.
2. When about twice the standard dose was administered daily for two consecutive days, an expulsion rate of 100% was obtained both from ascarids and hookworms. No effect was observed upon tapeworms.
3. When about twice the standard dose was administered only once, ascarids were removed from dogs at a rate of 72.4%, on the average.
4. Soft feces or diarrhea was seen during the period of administration and a few days which followed. No other side effects were apparent, in spite of the cholinesterase-inhibiting action of the organic phosphorous preparation.
5. Clinical examination made it clear that dogs harboring whipworms (20 cases) and those infected with hookworms (3 cases) had turned to be negative for parasite egg excretion, without exception, and that the state of feces had been improved conspicuously.

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Bayer L 13/59 (Neguvon), an organic phosphate with low toxicity, has frequently been used for the treatment of canine demodecosis in recent years. There are, however, few studies on the effect of this compound on the cholinesterase activity of canine serum and tissues. The present investigation clarified that treatment with dose of 50 mg/kg of the compound induced a reduction (40-50%) in cholinesterase activity, a rapid return to normal, and no toxic manifestations. The method widely adopted of administering three doses on the 1st, 3rd, and 9th day of 50 mg/kg each was found to be of the same effect as the repetition of the onedose method mentioned above. This method brought about no cumulative toxicity and was proved to be safe for the purpose of treatment.

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A bacteriological survey was carried out on 230 mice and rats trapped in eight areas in Kami County, Kochi Prefecture. A total of 203 strains were detected from the rectum of these animals, SS medium being used. Based on biological properties, they were classified as follows (the number of strains in parentheses): Escherichia coli (88), Cloaca (23), Citrobacter (18), Proteus vulgaris (12), Morganella (8), Providencia (8), Proteus mirabilis (7), Klebsiella (3), Rettgerella (3), and others.
Of 42 strains of E. coli examined serologically, ten were found to be pathogenic and consisted of three strains of 0-119, two strains of 0-126, and one strain each of 0-25, 0-26, 0-75, 0-111, and 0-112. These pathogenic organisms had been harbored by 15 Norwegian rats, an Alexandrian rat, a roof rat, and a mouse.

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In March, 1962, on a poultry farm in Osaka, a certain respiratory disease occurred in a 50-day-old chicken flock. The outstanding symptoms were gasping, rale, and coughing. Some chickens showed gasping, with the head extended and the beak open. The fatality rate was 20 per cent in the flock. The mucous membrane of larynx and trachea was covered with a yellowish caseous exudate or blood-stained exudate. In some chickenss blood covered the mucosa.
Histopathologically, intranuclear inclusion bodies were found in the tracheal epithelial cells. By intratracheal inoculation of the tracheal material, the same respiratory symptoms were produced and serial passage through the trachea was possible. A certain virus was isolated by inoculation of embryonated eggs in CAS and on CAM. There were white grayish areas or pocks produced by the virus and intranuclear inclusion bodies in sections of the membrane. In chicken inoculation of the virus, intranuclear inclusion bodies were found in groups of tracheal epithelial cells.
Neutralization test was carried out, by inoculation on CAM, with the virus and serum from chickens inoculated with the isolated virus. The result was successful.
The sinus of the chicken was sensitive to the isolated virus.
In conclusion, the virus isolated on CAM was infectious laryngotracheitis virus. Therefore, the respiratory disease affecting this chicken flock was diagnosed as infectious laryngotracheitis.

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