The effects of methyridine (Promintic, an injection formula) on canine helminths were evaluated in 94 dogs.
Trichuris vulpis was almost completely removed with a dose of 36mg of active ingredient per kg of body weight. The drug showed a considerably high efficacy against Ancylostoma caninum at a dose rate of 135mg/kg or higher. It appeared to have some action on adult Dirofilaria immitis with two doses of 200mg/kg given 16 days apart. A dose of 200mg/kg showed only low efficacy against Toxocara canis, and no action on Dipylidium caninum and Diphyllobothriumerinacei.
When subcutaneosly injected, the drug gave rise to such systemic reactions as salivation, vomiting, ataxia, and lameness at a dose of 75mg/kg or higher, but no side reaction at a dose of 50mg/kg or lower.
The intraperitoneal injection of the drug produced transient systemic reactions at such a low dose as 20mg/kg. The intramuscular injection of it developed local reactions at a dose of 36mg/kg or higher. Accordingly, these routes seemed to be unsuitable for the treatment of dogs. The subcutaneous injection done on the neck or dorsal area resulted in pain at the site of injection immediately after injection with such a low dose as 5mg/kg, and produced swelling or dermatitis at a dose of 18mg/kg or higher. No local reaction was produced with a dose of 45mg/kg or lower administered subcutaneously on the lateral side of the thigh.
It is concluded that a dose of 36mg/kg of methyridine (0.04cc/kg of Promintic) administered subcutaneously on the lateral side of the thigh has a high practical value in removing whipworms from a dog.

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Serum neutralizing antibody titers were observed in antibody-negative dogs for one year after the injection of live virus canine distemper vaccines. The titers showed wide range differences according to individual susceptibility. A low percentage of dods which had initially responded to the vaccine had no antibody titers a year later.
Two different brands of commercially available vaccine (vaccine A and B) were tested for antigenicity. As a result, vaccine B proved to have a higher serum antibody titer in inoculated animals than vaccine A. There was no difference in antibody response between pure bred and mongrel dogs, or between the sexes.
Antibody titer at the time of vaccination proved to be a main factor to determine whether the immune response occurred or not. There was no relationship between age and immune response when a dog was free from serum neutralizing antibody. A second injection had no booster effect on dogs which had a sufficient titer of serum neutralizing antibodies.

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Specimens were collected at 14 points of the.Japan Sea off Tottori Prefecture almost once a month over period from April, 1964, to November, 1965. The farthest point of the fourteen was located 28.0 kilometers off the shore. The specimens consisted of 657 water samples collected from zero-, ten-, and fifty-meter strata of the sea water and 209 plankton samples harvested from a vertical depth of water of 30 meters. They were examined for the presence or absence of Vibrio parahaemolyticus.
1. The organism was detected from 61 water and 11 plankton samples, or from 8.3 per cent of the total specimens.
2. The distribution of the organism was higher in such points as located 10.1 to 28.0 kilometers off the shore than in points 2.7 to 8.7 kilometers far from the shore.
3. The organism was detected principally during a period from May to September of the year.
4. Frequency of detection was the highest in the water samples collected from the ten-meter stratum and the lowest in those from the fifty-meter one of all the water samples from the three strata.
5. When analyzed from chlorine ion concentration, the rate of detection was high from the sea water at a concentration of 17.1 to 17.5 per mille, moderate from that at 17.6 to 18.0 per mille, and low from that at 18.1 to 19.5 per mille.
6. Of the 72 strains isolated, 18 strains (25.0 percent) were classified into biotype 1 and the remaining 54 into biotype 2.

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A 7-year-old cow of the Japanese Black breed which had been raised on a farm in Hiroshima Prefecture since purchased at livestock market at 3 years of age was slaughtered at an abattoir.
At autopsy, so-called pearl nodules were found to have developed densely on the great omentum and pleura. The mesenteric lymph node was enlarged to the size of fist. Nodules of miliary to walnut size were present abundantly on the cut surface of the lung.
Histopathologically, a typical picture of miliary tuberculosis was seen. Caseous degeneration was observed in the liver and the lymph node of the hilus of the lung. There were no remarkable changes in the mammary lymph node.
Bacteriologically, tubercle bacilli were isolated from cultures of lung, lymph node of the pulmonic hilus, liver, and mammary lymph node. They were identified as those of human type from their properties and the results of animal inoculation tests.
The man who had raised this cow and his family had no history of tuberculosis. It was impossible to affirm that this cow had any chance to get in touch with a human tuberculous patient. Therefore, the source of infection was unknown in this case.

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1. When 23 three-month-old chickens were inoculated with fowl pox vaccine, all of them acquired complete immunity.
2. Three-day-old chicks were vaccinated by the follicle method and then challenged 1-3 months after vaccination. Five of 18 birds were immune completely for challenge, but the rest allowed more or less pock formation on the challenged area.
3. When 12 chickens were vaccinated twice, at 3 days and some time between 20 and 40 days of age, all of them indicated almost complete immunity.
4. Chickens of various ages, extending from 3 days to 2 months, were vaccinated and then challenged. One of 6 chickens vaccinated at 3 days of age was immune completely. All the 12 chickens vaccinated some time between 14 days and 2 months of age were completely immune. In short, the vaccination at 14 days of age or later was especially effective.
From these results, it seems necessary to perform booster vaccination to prevent chickens from the respiratory disease induced by fowl pox virus, so far as 3-day-old chicks are concerned.

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