Over a period from September, 1965, to November, 1967, left displacement of the abomasum was observed in 17 Holstein-Friesian cows, 16 of which were patients from 3 towns adjacent to the University Farm, which is located 60 miles northeast of Tokyo. There was nothing noticeable in diet and circumstances that might have been predisposing factors in these cows. there was no apparent seasonal relationship to the development of the disease.
The onset of symptoms was approximately 11 weeks to 1 day ante-partum in 7 cases, immediately following parturition in 2 cases and 1 to 41 days postpartum in 8 cases.
Simultaneous auscultation and percussion was proved to be the most rapid and reliable method of diagnosis. A high-pitched resonant ping sound was heard in all cases when percussion was done on the last ribs with the percussion hammer. In 6 cases, endoscopy via the left paralumbar fossa was attempted and resulted in confirmation of displacement.
Bilateral flank approach associated with omentopexy to the right abdominal wall was used for the treatment of 12 cows in the standing position. Right paramedian approach with omentopexy to the ventral abdominal wall was performed in 4 cases. Twelve of 16 cases recovered uneventfully. Of those 12 cows, one calved twice and four once subsequent to surgery; seven were pregnant and three not pregnant at the end of November, 1967; two were sold. Four cows were destroyed after surgery because of complications, such as disseminated pulmonary abscesses originated from a piece of wire perforating the esophagus, adhesion of the abomasum with the left ruminal wall, aggravated suppurative mastitis, and displacement of the dilated abomasum to the right flank following devouring of a sheet of blanket.
In the remaining one cow, in which omentopexy had not been performed on account of spontaneous replacement of the displaced organ, the condition relapsed 18 days later. Then the cow was subjected to surgical correction by practitioners.

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The relationship between the environment within a milk plant and contamination of the produced milk with psychrophilic bacteria (PB) was analyzed in a plant where the UHT system of pasteurization was carried out.
1. The raw milk used contained about 10₈ PB. No PB were detected from any sample of sampling-cock milk or surge-tank milk immediately after collection or at 7 days of storage at 5 to 7°C. Samples of bottled milk were found to harbor 10₁ PB even immediately after collection.
2. Contamination was hardly observed in any machine after routine rinsing and disinfection. Exceptionally, the filling valve was proved to have been contaminated remarkably. Even a sample collected at it 4 hours after the beginning of operation contained 10₁ to 10₂ PB, which belonged to the genera Pseudomonas and Micrococcus. An adequate sanitary control was effective to reduce the contamination to a great extent.
3. No PB were detected at all from samples collected from the washing bottle or the cap.
4. The atmosphere within the plant harbored 10, to 10₂ PB, which belonged mostly to Pseudomonas.
5. Rather marked contamination was recognized at the fingers and clothes of employees, the floor of the plant, the conveyer, the hood of the bottling machine, and the frames of windows. Many organisms of Pseudomonas and Micrococcus were detected from the fingers and clothes of employees and the floor of the plant.
6. These results seem to indicate that the PB harbored by bottled milk are derived from the filling valve, the atmosphere of the plant, and such environmental conditions of the plant closely related to these as

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遠心管内遊出法による牛肺虫第1期子虫検出の基礎試験に続いて, 本検査法とベールマン氏法, カワラ培養法などとの比較を試み, 応用的見地から牛肺虫実験感染牛について子虫の排泄消長を調べた. また, 牛肺虫病の2発生例について本検査法を実施した.
比較試験では遠心管内遊出法はベールマン氏よりも検出子虫数が多く, カワラ培養法よりかは検出率が極めて高かった. 実験感染牛のPrepatent periodは25～27日, 最高子虫数は感染後36～37日目であった. その後, 子虫は次第に減少したが, 感染後から子虫消滅までの期間は概して50～65日であった.
北海道, 豊富村の1牧野における発生例のうち送付された材料は病的時期や郵送期間における子虫の自然減少などから, 必ずしも好適ではなかったが13頭中9頭から子虫を検出した. 東京都下三宅島牧野の発生は多くの牛が明瞭に牛肺虫病の症状を示したが, 初放牧幼牛97頭中85頭から種々な程度の子虫数を検出し, 最高LPG1366であった. 放牧経験牛の感染率は低く子虫数も少なかった.
これらの成績から, 遠心管内遊出法によって牛肺虫病を正確かつ容易に診断しうるものと思われる.

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