Chrome-sulfuric acid was added to a fecal sample to decompose and dissolve the fecal contents, so that the counting of coccidial oocysts might be made with ease. 1. The chemical used was a mixture of equal volumes of 20 % sodium bichromate and concentrated sulfuric acid. 2. It was desirable to finish the oocyst counting within 30 minutes after the chemical treatment in order to prevent the oocysts from being disintegrated. 3. A fecal sample unfiltrated and containing a large amount of residual feed sometimes became foamy. This disadvantage could be prevented by increasing the amount of chrome-sulfuric acid to be added.
A survey was conducted on the source of contamination with psychorphilic bacteria in a milk plant where ultra-high temperature pasteurization was carried out. 1. Psychrophilic bacteria were detected from all the samples tested of city milk stored at 5°C for 7 to 10 days. They were mostly of the genus Pseudomonas. 2. They were found in such portion of the process as farther than the bottling machine. 3. The bottling machine was negative for the swab test of psychrophilic bacteria immediately after swabbing, but was positive after storage at 5°C for 10 days. The capping machine was negative in both cases. 4. The falling bacteria studies in the bottling room included Pseudomonas at the rate of 14.9%.
An outbreak of so-called bovine influenza occurred throughout Japan over a period from October, 1968, to April, 1969. A total of 42, 756 cattle were involved and 153 cattle died, were condemned or sacrificed. The case fatality rate was 0.40%. The main symptoms manifested consisted of a fever(39.0-42.0°C), polypnea, coughing, abundant rhinorrhea, lacrimation, salivation accompanied by copious foams and occasionally by blood, and leukopenia. They almost disappeared after about 10 days. Autopsy revealed severe pulmonary emphysema and hepatization in almost all cases. Spontaneously infected cattle were examinedserologically for neutralizing or hemagglutinationinhibiting antibody against known viruses of bovine origin. They had no relations to bovine epizootic fever, Ibaraki, or infectious rhinotracheitis virus. Some of them showed a significant increase in antibody titer against bovine diarrhea virus, bovine adenovirus type 6, and parainfluenza virus type 3. None of these viruses, however, seemed to be incriminated for the present outbreak. The etiology of the so-called bovine influenza remained unknown.