豚赤痢の発生豚群, 健康豚群およびSPF豚群の主として糞便についてVibrio coliの検索を行ない, つぎの成績を得た.
1) 糞便の5～10倍乳剤を孔径0.65μのmilliporeフィルターで沢過すると, 乳剤中のVibrio coliは選択的に沢過され, ほとんど純粋に分離培養された.
2) 豚赤痢の発生豚群 (197頭) におけるVibrio coliの検出率についてみると, この豚群のうち発症豚 (76頭) では75.0%(57頭), また病豚との同居豚 (121頭) では30.6%(37頭) であり, 平均47.7%(94頭) の検出率であった. これに対し, 健康豚群 (105頭) では11.4%(12頭) の検出率であった. いっぽう, SPF豚群 (32頭) から該菌が検出されるようなことはなかった.
3) 豚におけるVibrio coliはほとんど大腸に限局して存在しており, 原則として実質臓器に侵入増殖するようなことはなかった.
4) 豚赤痢罹患豚の腸内菌叢をみると, 上部消化管におけるEscherichia coliの増殖が目立ち, その内容物1g中の菌数は10⁷個内外に達していた.
5) わが国の豚から分離されたVibrio coliは硫化水素産生能および1%グリシン耐性能から, I. 硫化水素産生 (+), 1%グリシソ耐性 (+), II. 硫化水素産生 (+), 1%グリシン耐性 (-), III. 硫化水素産生 (-), 1%グリシン (-) の3生物型に分けられた.

抄録全体を表示

In August, 1966, blindness began to occur collectively among baby chicks caused by some commercial feeds in many districts of the western part of Japan. It usually affected some strains, such as Kimber K 44, Babcock B 390, and Warren Sex-Sal-Link F, but not a White Leghorn Strain.
The present experiment revealed that blindness had been provoked among chicks given feeds supplemented with ammeline and fish meal.
1. A specific substance causing blindness in baby chicks was an unknown organic compound contained in some fish meal. It had been added to the fish meal unlawfully as a false protein factor to increase the nitrogen content.
2. Regardless of the specific substance contained in the feed blindness was provoked in baby chicks by feeding a diet containing ammeline.
3. The clinical symptoms and gross and microscopic findings of the retina of blind chicks fed some fish meal were in accord with those of blind ones fed a diet supplemented with ammeline.
4. Blindness and dilatation of the pupil were observed in chicks 48hours to 7days after oral administration with ammeline added to a diet at more than 0.5%. Abnormal waves were found in the electroretinogram of chicks 24hours after administration with ammeline at a level of 3%.
6. Ammeline had a specific affinity to the retina of the chick. Early histological examination revealed the disappearance of fuscin granules from the retinal epithelium, conspicuous edema, degeneration and destruction of the visual cells, and retinal detachment. So that the chicks lost their own sight. Late examination revealed the lesions of the retina replaced partly by proliferated connective tissue fibers. The diminution and disappearance of paraboloid glycogen from the inner segment of the visual cells were demonstrated by using Bauer-Feulgen's method for glycogen.

抄録全体を表示

When vaccinated chickens of a flock were infected with Asian type Newcastle disease (ND), some of them showed a nervous syndrome. Pathological examination was made on 36 infected chickens of 9 flocks.
1. Typical gross lesions of Asian type ND were observed in non-vaccinated flocks. On the other hand, characteristic central-nervous-system (CNS) lesions occurred in the vaccinated flock.
2. CNS lesions were found in the cerebral hemispheres, midbrain, cerebellum, and medulla oblongata. Disseminated non-purulent encephalitis was accompanied by primary or secondary necrotic foci.

抄録全体を表示