Four green monkeys were incoulated intracerebrally with 10
5.0-10
6.0TCD
50of the low virulent Japanese encephalitis virus, the T37
- (S
-) strain, but manifested no clinical symptoms. They were held under observation for 14 days and sacrificed for histopathological examination of the central nervous system. As a result, mild changes were noticed in one of two animals inoculated with 10
6.0TCD
50. The other three animals were perfectly free from pathological changes. When sacrificed, all the inoculated momkeys were found to have neutralizing antibody in the blood. It was concluded that the virus tested was much attenuated Japanese encephalitis virus.
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