Biochemical and hematological examinations accompanied by the tuberculin test were carried out in two cattle herds infected with bovine tuberculosis in Hyogo Prefecture. The results obtained from them were evaluated in relation to pathological findings on slaughtered cattle. The following results were obtained. 1. The incidence of tuberculin-positive reactors in the two herds examined was 44.1%(15/34) and 16.3%(7/43), respectively. 2. When tuberculin-positive reactors with moderate tuberculous lesions were compared with tuberculin-negative reactors, red blood cell count and serum protein and γ-globulin contents were statistically higher in the former group. In contrast, albumin-globulin ratio, albumin content, Hayem's solution consumed in the Gros reaction, and GPT value were statistically higher in the latter group. 3. Statistically significant reverse interrelationships were observed among the extent of tuberculous lesion, alubumin-globulin ratio, and Hayem's solueion consumed in the Gros reaction. 4. Cattle with such abnormal hematological findings as mentioned above were slaughtered systematically in connection with the results of tuberculintests in a severely infected herd. As a result, the rate of incidence of tuberculin-positive reactors decreased from 44.1% in 1969 to 2.9%(1/34) in 1971. From these results, biochemical and hematological examinations were regarded as valuable supplemental methods to detect tuberculosis in herds. Discussion was made on these findings.