The rate of swine positive for C-reactive protein (CRP)-like substance in serum was examined. It was 84% in 50 hogs raised on general farms, 52% in 25 hogs reared on hog farms, 55% in 20 secondary SPF pigs, and 47.6% in 21 piglets on general farms when determined by the capillary precipitation method with commercial anti-CRP rabbit serum for human use. There was a tendency for the positive rate and the grade of positivity to be low on farms showing low microbial contamination. The positive rate was high in swine with renal hemorrhage, pneumonia, gastritis, enteritis, epicarditis, and septicemia. It was low in swine with lesions restricted to a single organ and high in those with lesions spread over the lung and kidney or more than two other organs.
Swine pox appeared in 207 pigs 30-70 days old on a hog farm in Mie Prefecture in 1978. Many lesions were seen on the skin. They recovered in 20-30 days. Histopathologically, eosinophilic intracytoplasmic inclusion bodies and intranuclear vacuoles were seen clearly in hyperplastic cells in the stratum spinosum of the epidermis. Electronmicroscopically, many pox virus particles were seen in the cytoplasm of the epidermal cells. From those skin lesions, viral agents were isolated after six passages in PK-15 cells. No such agents were isolated in HeLa, CEF, or CAM. They were sensitive for ether treatment.
Eighteen cows weighing about 500 kg each died suddenly on Farm N of fattening beef cattle over a period beginning with December 18, 1977. Most of them suddenly manifested recumbency, pyrexia, anorexia, and dyspnea, without showing any precursor symptom, and succumbed within 12 hours. Autopsy revealed purulent meningo-encephalomyelitis accompanied with thrombosis and bacterial embolism, or embolic meningo-encephalitis reported in other countries. A diagnosis of purulent meningo-encephalomyelitis caused byHaemophilus somnuswas made on these cattle. The disease came under control after preventive antibiotic administration was done on the other cattle of the farm.
SPF chicks 2 and 21 days old were used to examine horizontal transmission of the FK-78 strain of infectious bursal disease (IBD) virus. Viral discharge into feces was observed in chicks over a period from 1-2days to 6-7days after oral inoculation with the virus.It was fbund in chicks exposed to contact infection with these chicks immediately after inoculation, over a period from 4-5 days to 10 days after inoculation. The largest amount of virus was recovered from the inoculated chicks 3-4 days and from the exposed chicks 6-7 days after inoculation. AGP or neutralizing antibody was noticed in the serum of all the inoculated and exposed chicks 14 days after inoculation.
Serum a-fetoprotein (AFP) levels in several species of animals were measured by the polyethylene glycol method of radioimmunoassay for human AFP. The serum AFP level was less than 15 ng/ml in normal adult dogs, cats, cows, goats, horses, mice, rats, guinea pigs, rabbits, chickens and human beings. In newborn puppies, kittens, and calves it was high at birth and decreased rapidly within 24 hours and then gradually with an advance in age. In newborn rats and chicks it was low at birth and decreased gradually with an advance in age. No significant changes occurred in the maternal serum AFP level of normal pregnant dogs and cows in any stage of pregnancy. Radioimmunoassay seemed to be available for investigation of changes of AFP level in animals in which AFPs were strongly cross-reactive with the anti-human AFP.