A pregnant sow gave birth to 12 piglets about one month after introduced into a farm. The young died suddenly with symptoms of blooding from the anus and prostration within 19 hours. Immediately after death autopsy was performed on four of them to collect materials for microbiological and pathological studies.
Clostridium perfringens was isolated from all the intestines and C. sordellii and C. paraputrfcum were from some intestines in the order of 10
6-7/g. These microorganisms also were found in some parts of the animal body. By the neutralization test with anti-
C. perfringens type A serum, 15
C. perfringens strains belonged to
C. perfringens type A. Their toxin titers reached 0.05 to 2.5 u/ml by Evans's method. Toxin was also shown in the intestine of a piglet. There was, however, discrepancy between the presence of toxin in the intestine and the isolation of toxic
C. perfringens type A. Pathological specimens of the intestine showed the image of necrotic enteritis. The disease was diagnosed as necrotic enteritis with bacteremia with
C. perfringens accompanied with
C. sordellii,
C. paraputrificum, etc. Epidemiological discussion was made, since a similar disease occurred 6 years before.
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