Dogs were immunized with mamushi venom toxoid which had been inactivated with hydrochloric acid and formalin. In them the dose of the toxoid and antihemorrhagic antibody levels were investigated. As a result, it was revealed that the antibody was maintained for 6 months by injections with 2mg of the toxoid at the beginning, 0.5mg in the third week, and 1mg in the third month.
Five fattening cattle weighing 270-280kg each dies suddenly on a farm in September and October, 1980. Two of them were subjected to autopsy, which revealed congestion and hemorrhage in the brain and the mucosa of the small intestine. Histologically, they exhibited suppurative meningitis with cerebral thrombosis, and necrosis and suppurative inflammation of the mucosal epithelium of the intestine.Haemophilus somnuswas isolated from the brain and other organs in almost pure form, andClostridium perfringensfrom the contents of the small intestine.
of Hokkaido. It was once characterized by hyperemia of the conjunctivae and lacrimation, but, in these 2years, by keratitis with white-turbid conjunctivae. It frequently attacked relatively young cattle. Etiologically, Moraxella boviswas isolated from 17 of 65 animals with the remarkably whiteturbid cornea, andMor. nonliquefaciensat high frequency from animals presumed to be in the early stage of disease and healthy cattle.Branhamella catarrhalis and Gram-positive cocci were also isolated from animals involved in chronic infection with vascularization in the white-turbid cornea.Mycoplasma spp. were found in most animals manifesting various symptoms and some apparently healthy ones, andUreaplasma spp. in one infected and 8 healthy animals.
Heart infusion medium was proved to be an optimal transportation medium forHaemophilus equigenitalis, since this organism survived in it within a range of 31 to-20°C. Optimal media were a medium containing 400μg/mlof streptomycin for cultivation of samples collected from the clitoral fossula of convalescent or asymptomatic mares and the same medium containing no streptomycin for cultivation of materials collected from vaginal exudation and uterine cervix. The MIC of streptomycin was 500 600μg/mlfor 20 strains ofH. equigenitalisisolated from cervical swabs of mares spontaneously infected with ocntagious equine metritis.
Two calves were born normally in January and February, 1980, respectively. They showed ataxia, obvious cerebellar hypoplasia, and a high postcolostrous titer of bovine viral diarrhea-mucosal disease (BVD-MD) virus neutralizing antibody. Fetal infection was confirmed in a clinically healthy calf born at the same time with one of them. A survey revealed that the positive rate of BVD-MD virus infiltration was relatively high among cattle grazed on pasture.
Retinodialysis was found in 2 left eyes of 2 cows when direct ophthalmoscopy was carried out on both eyes of 1, 166 dairy cows, showing a morbidity rate of 0.17%. The 2 eye were affected with total and partial retinodialysis, respectively. Since cattle have a monocular vision, the 2 cows seemed to have no difficulty in locomotion.