Two methods of hemolysis test with hydrogen peroxide (one devised by Ikehata et al. and the other modified by the present authors) were evaluated to estimate the concentration of vitamin E (VE) in swine serum.
In experiment 1, 25 blood samples were obtained from 9 gilts fed a VE-poor, VE-rich or ordinary diet for about 1-3 months. When estimated by the method of Ikehata et al., the hemolysis rate averaged 58%, ranging from 3 to 93%, in them. The serum concentration of VE as α-tocopherol in the same samples was determined by the method of Abe and Katsui with high speed liquid chromatography. It averaged 0.18mg/d
l, ranging from 0.03 to 0.52 mg/d
l. There was a highly significant negative correlation between the hemolysis rate (X) and the VE level (Y) in these samples (Y=-0.005X+ 0.464, r=-0.93, P<0.01).
In experiment 2, 10 blood samples were collected in heparinized vacuum tubes from 8 gilts fed diets at different VE levels for 5-14 months. In them, the hemolysis rate by the method of Ikehata et al.(X) and that by the authors' modified method (Y) almost coincided with each other. A highly significant correlation was observed between them (Y=0.995X+0.98, r=0.995, P<0.01).
In conclusion, the modified method seemed to be simpler and more effective than the original one for the screening test of VE deficiency in swine.
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