A Cassou gun made in France for artificial insemination was placed into a sterile plastic tube to prevent microbial contamination. The tube was punctured when it reached the uterine cervix. In one method irrigation was done with Ringer's solution containing 30% bovine serum or with TCM-199 solution. Then tansfer was conducted with Ringer's solution containing 50% bovine serum. In the other method irrigation was done with Eagle's MEM and transfer with BMOC-3 solution. As a result, the rate of pregnancy was 57.1 and 60.0% in the former and latter method, respectively. Bovine ova were collected 7 or 8 days after estrus of superovulation and stored in Ringer's solution supplemented with 50% bovine serum. They were transferred by 7 operators into the uterine horn ipsilateral to the corpus luteum-bearing ovary in cows 7 or 8 days after estrous. It was concluded that transfer of fertilized ova with the Cassou gun was practicable in cows.
The efficacy of flubendazole was examined against natural infection with gastrointestinal nematodes in dogs. Eighty-one dogs infected with one or more nematode species received an oral dose of 20 to 30 mg/kg/day for 1 to 3 days for ascarids and tapeworms and for consecutive 3 days for hookworms and whipworms. Sixty-five dogs were examined coprologically during 4 to 54 days after the treatment. Sixteen dogs were killed 10 to 30 days after the treatment and examined for residual worms. Of 37 dogs infected with ascarids, 35 (94.6%) became free from the parasites. Of 25 dogs which had received a single dose, 23 (92.0%) became free. Of 39 dogs infected with hookworms, 39 (100%) were cured. Of 29 dogs infected with whipworms, 25 (86.2%) were cured. Of 4 dogs infected with tapeworms, none were cured. No side effects were observed in any dog.
The intestinal adenoma complex (IAC) was detected in 30 swine, or in 0.02% of the swine examined by meat inspectors over one year ending in October 1981. Of the 30 swine, which were 6-month-old hogs, 14 were affected with porcine intestinal adenoma (PIA), 6 with regional ileitis (RI), 5 with necrotic enteritis (NE), one was with proliferative hemorrhagic enteritis (PHE), one with RI/PIA, and 3 were with an unknown type of IAC. Histopathologically, adenomatous hyperplasia of mucosal epithelial cells was a common finding. In addition, fibrosis of the mucosa and enlargement of the muscle layer were found in RI, pseudomembranous formation was in NE, and mucosal hyperemia, hemorrhage, and severe inflammatory reaction were in PHE. Bacteria looking like those of Campylobacter sputorum subsp. mucosalis were detected from the cytoplasm of hyperplastic epithelial cells in the swine affected with these types of IAC.
A disease of high fatality had been prevalent among chicks on one broiler farm since the time of introduction into the farm. A flock of 11, 000 chicks was reduced to about 69% before it was shipped to the market. Dead birds presented edematous changes and scattered yellowish white nodules of pinhead size in the lung. A number of organisms of Rhizopus chinensis Saito were isolated from the lung in all the birds examined. Hyphae presumed to be those of this species were also observed histopathologically in the lung. A diagnosis of pulmonary phycomycosis was made in these birds.
Osmotic fragility of erythrocytes was estimated by coil planet centrifugation (CPC). In cattle free from Piroplasma infection and abnormal hematological fiondings, it was 122.5±6.8, 99.9±7.3, and 84.0±5.4 mOsm as HSP, HMP, and HEP, respectively, regardless of age. In cattle infected with Piroplasma (+++), HEP was higher in level, but the range of hemolysis was lower than in those free from the infection. Raising cattle exhibited low levels of HSP and HMP at 5 months of pasturing and almost the same levels of them as those reared in the barn at 7 months of pasturing. The estimation of osmotic fragility of erythrocytes by CPC was considered to be useful for the clinical diagnosis of hematological disease in cattle and health administration of cattle reared in the pasture.
Seventeen young boars were exposed to spontaneous infection with Japanese encephalitis virus (JEV). They manifested scrotal edema, which disappeared about 10 days later, a rise in HI antibody against JEV, two-peak pyrexia, and anorexia. Histopathological examination revealed subacute orchitis, epididymitis, funiculitis, and nonpurulent encephalitis. These changes were marked 1-5 days after the appearance of scrotal edema. Organization of the testis, low and insufficient spermatogenesis, and nonpurulent encephalitis were noticed over a period from 7 to 41 days after the appearance of scrotal edema.
Lung fiuke eggs were detected from the feces of a male Yorkshire terrier 2 years and 4 months old raised in an urban area known to be free from contamination with this fluke. They were confirmed tobe of Mivazaki lung flukes.The dog was administered orally with 25mg/kg of bithionol twice a day for 4 consecutive days. Soon the eggs turned to be negative and coughing disappeared. No eggs were detected even 12 months later.