Dogs infected with the cestode, Mesocestoides paucitesticulus, generally showed mild clinical signs. Occasionally, soft stools occurred to them when a small number of cestode proglottids were passed. Diarrhea occurred when many proglottids were shed in feces. No changes were detected in pulse, respiration and body temperature. Vomiting, abdominal pain, or anorexia was observed. No significant changes were found in any blood figure either. Kamala, arecoline, bunamidine, dichlorophen, and bithionol preparations were used for treatment. After the singly or combined administration of these preparations, cestode proglottids were discharged into feces, but cestodes with scoleces did not. The arecoline preparation was effective when injected rectally with 2, 000 ml of warm water at 42°C.
An attempt was made to clarify the absorption of antibody in colostrum by prostaglandin F2α-delivered premature calves. Six pregnant cows were treated with prostaglandin F2α (PGF2α) to induce premature parturition. As a result, 6 healthy calves were born from them at 257 to 277 days of gestation. They were separated immediately from their dams after birth and fed 500 to 3, 500 ml of colostrum first milked 6 to 11.5 hours later. Additional 500 to 3, 000 ml of secondly milked one was fed 3 to 11.5 hours after the first ingestion. Then they were allowed to ingest fresh cow's milk ad libitum twice a day. Sera were collected from them before and 3, 6, 9, 12 and 24 hours after ingestion of colostrum and then daily until 7 days of age. Two sera were also collected from each dam before and after parturition. Neutralizing antibody against parainfluenza type 3 virus (PI-3) was checked as a representative of antibodies by the neutralization test. No PI-3 antibody was detected from the precolostral sera of any calf. It increased rapidly to reach a maximum level about 12 hours after ingestion of colostrum. This level was maintained for more than 6 days. It was equal to the level in the dams during the puerperal period. These results indicate that the PGF2α-delivered premature calves absorb antibodies from colostrum, so that the concentration of antibody in the calf blood may attain the same level as that in their dams about 12 hours after ingestion of colostrum.
A daily dose of 1g of a pantothenic acid preparation per head, which contained 100mg/g of pantothenic acid, was added to the milkreplacer and given continually to suckling male calves during a period from 10-15 days of age to weaning. In control groups, bifidobacteria, Clostridium perfringens, lactobacilli, enterobacteriaceae and streptococci decreased with the lapse of time. In the treated groups, however, the decreasing tendency of bifidobacteria and lactobacilli was noticed significantly late, C. perfringens and staphylococci decreased in frequency of appearance, and the ratio of dry matter of feces increased to suppress the occurrence of diarrhea slightly. These results indicated that the administration of pantothenic acid to calves during the nursing period suppressed the occurrence of diarrhea and validly controled the balance of the intestinal flora.
Embryonated nematode eggs, which were almost the same in size as those of hookworms, were detected by the flotation method with saturated NaCl solution from the feces of 3 kittens in Hakone-Machi, Kanagawa Prefecture, Japan, in October, 1981. Cultivation of the feces by Harada and Mori's method revealed filariform larvae and rhabditiform females and males, which seemed to belong to the genus Strongyloides. The nematodes obtained were assigned to Strongyloides planiceps Rogers, 1943 by the morphological characteristics of free-living larvae and adults and parasitic females, and of eggs passed in feces, and by such ecological features as the habitat of parasitic females and the prepatent and patent periods. S. planicepsseems to be widely distributed in Japan. So the eggs of this species must be distinguished from those of hookworms, which are similar in size and morphology when embryonated, by fecal cultivation for the exact diagnosis of feline strongyloidiasis.
Antibody against hog cholera virus was examined, the virus-neutralizing test, in market pigs from 1976 to 1982, and in breeder pigs in 1981. The positive rate of market pigs ranged from 86.9%(193/224) to 95.4%(274/287) in each year, indcating that the vaccination seemed to be well done in Shizuoka Prefecture. The maternal antibody-titer of piglet as estimated from breeders, indicated that 86% of the pigs took hog cholera live vaccine at 23 days of age, and that 77.2% had been protected from the field infection. The rate of vaccinated pigs, as estimated from the numbers of breeder pigs and inoculated pigs, was above 90% in each year. Especially high levels (96.8-98.1%) were shown in 1980 and later.
A four-year-old female Ainu dog was affected with both exocrine and endocrine disturbances of the pancreas, including polyphagia, steatorrhea, severe emaciation, hyperglycemia and a decrease in glucose tolerance. Severe atrophy of the pancreas with acinar atrophy and a marked decrease in B cells in Langerhans' islets was observed pathologically. The cause of the present conditions was unknown.