Abortion occurred to three of 5 pregnant mares on a draft horse farm near Ebetsu, Hokkaido, over a period from October to December, 1981. One of these mares showed a sign suggestive of the occurrence of abortion. Streptococcus zooepidemicus was isolated from swab specimens collected from 2 aborted fetuses and the external orifice of the uterus of their dams and the mare showing the abortive sign. The total amount of urinary estrogen was small in the mares affected before the appearance of this sign and the occurrence of abortion. This sign disappeared in the mare when antibiotics were administered by the intravaginal route and persistent progesterone preparation was injected intramuscularly. Then the mare gave birth to a normal foal. S. zooepidemicus seemed to have played an important role in the incidence of abortion in the mares involved. It might have been originated from a mare suffering from pyometra in the same stable or a big flood attacked the farm some time before. Transmission by the stallion was negatived.
A total of 66 bovine embryos were frozen in glass ampoules (6 × 70mm) and stored in liquid nitrogen. Then, they were thawed rapidly in water at 37°C. The storage medium used was Dulbecco's PBS + 25% calf serum containing 1.0M glycerol. Embryos in the storage medium were cooled from 35°C to - 5°C at a rate of 1.0°C/min. and seeded at-5°C. After seeding they were cooled to -36°C at 0.3°C/min. or at 1.0°C/3 min. before being transferred directly to liquid nitrogen. As a result, 54 embryos were cultured for 24-48 hours, 12 embryos transplanted to 12 recipients, and 15 embryos (27.8%) survived after culture. Three recipients (25.0%) were pregnant and gave birth to 3 normal calves.
In October and November, 1982, three cows were affected with a disease with dysphagia as a main symptom in Saga Prefecture in the Kyushu region. They also suffered from salivation, vomiting, and mild pyrexia and died in 7 to 10 days. Autopsy revealed ulceration on the ventral surface of the tongue, scattered white spots on the mucosal surface of the esophagus, and hyperemia and ulceration on the mucosa of the abomasum. Histological changes noticed were coagulation necrosis of muscle fibers in the tongue, esophagus, and heart and necrosis and ulceration on the ventral mucosal surface of the tongue, Virus isolation was negative when it was tried in blood samples collected from one of the affected cows and one of six cows housed in the same barn as the affected ones, but in vain. Neutralizing antibody against Ibaraki virus was detected from all the affected cows and five of the 6 exposed ones. As a result, the disease was diagnosed as Ibaraki disease, the last outbreak of which was in 1960 in Japan.
Streptococcal meningitis broke out among piglets 40 100 days old on a farm in Mie Prefecture over a period from February to December, 1980. It was started with pyrexia over 40°C, ataxia, and crouching like dogs. Affected piglets lay on the side, trembled, and paddled with all the four extremities. The main autopsy finding was thick opalescent turbidity of the meninges. Histological examination revealed purulent meningitis of the central nervous system. Streptococci were isolated from the brain, lung, liver, spleen, kidney, and joint. Judging from their biological characters and serological typing, they were probably identical with Moor's group R streptococci.
Pseudomonas aeruginosa infection associated with cerebral necrosis broke out in broiler chicks. Of 13, 920 birds 2, 060 (14.8%) died with signs of depression up to 10 days of age. All the affected birds were female. Postmortem examination at 7 days of age revealed cerebral necrosis in four of 7 birds examined. Pseudomonas aeruginosa (Serotype G) was isolated in pure culture from the brain, liver and egg yolk in almost all the birds examined. The source of infection was not found.
A survey was conducted on the incidence of scabies in 61, 540 swine on 84 farms. The disease was found in 14, 406 swine (23%) of 80 farms (95%), including 60% of the breeding and 19% of the fattening animals. It was prevalent in early spring and late fall. Its signs were exacerbated in the former over a period from weaning to mating and in the latter around 40days of age. Satisfactory therapeutic effects were obtained by spraying 1: 300 or 1: 500 dilution of the low toxic organic phosphorus preparation, KWA-905, three times at 3-day intervals and once 2weeks later. The 1: 300 dilution was especially effective for fattening swine to increase the economic value.
Examination was made on pathological changes produced by the cestode, Mesocestoides paucitesticulus, in the final hosts, dogs, foxes and raccoon dogs, and in the second intermediate avian hosts, green pheasants. The final hosts revealed only slight catarrh in the mucous membrane of the jejunum. Bladder worms were usually found in the lung and gizzard of avian hosts parasitized by a small number of parasites. They were found in every part of the body when they were large in number. Histological examination revealed slight infiltration by lymphocytes in the lung and general congestion in the liver, but no remarkable pathological changes in the hepatic cells even when bladder worms invaded the parenchyma. No pathological changes were found in any other organ or tissue parasitized by bladder worms.
Three strains of bovine parvovirus were isolated from two herds of calves showing clinical illness. One of them was isolated from nasal secretions of a calf with respiratory symptoms in one herd. The other strains were isolated from fresh diarrheal stool specimens of two calves affected with respiratory and intestinal illness in the other herd. Pairs of serum samples were collected from all the calves of the herd, including the calves showing respiratory symptoms. They revealed a significant rise in antibody titer against bovine parvovirus.