A diagnosis of Salmonellosis was made on 130 swine of 557 with focal lesions in the liver and on 6 swine of 1, 655 with changes of septicemia in Osaka over a 6-year period beginning in 1972. Salmonella choleraesuis var. kunzendorf was isolated from 132 swine, S. choleraesuis from two, and S. typhimurium from the other two. The rate of Salmonella detection was 93.2% in the liver, 89.3% in the spleen, 49.2% in the Kidney and 39.6% in the internal iliac lymph node. Individually, Salmonella was detected from liver, spleen, and kidney of the same body in 45.8% of all the positive swine, from two of these organs in the same body in 36.4% and from one organ in 17.8%. Concurrent recovery of Salmonella from muscles was also successful in 11 swine of 14 detected the organisms from liver, spleen and kidney of the same individual, from two of these in 4 swine of 9, from one of these, however, none of 6 swine was recovered the organisms. Histologically, specific lesions (typhoid nodules) were consistently present in all swine identified, with the exception of 4 animals.
Canine parvovirus (CPV) was isolated in primary canine kidney cell and feline kidney (FK) cell cultures. It was detected from intestine and feces from eleven of 21 dogs clinically and pathologically suspected of CPV infection from 1980 to 1981. The isolated viruses were propagated with intranuclear inclusion bodies in primary FK cell and feline lung cell cultures and agglutinated porcine and feline erythrocytes. The serological properties of the isolated viruses were identical with those of CPV and feline panleukopenia virus. The serotype of the isolated strains was proved to be of monotype by the hemagglutination-inhibition (HI) test. In experimental infection with an isolate, clinical symptoms and pathologic lesions similar to those in field cases were induced. The virus was recovered from almost all the organs of dead pups. A serologic survey was conducted on 748 street dogs in various districts of Japan over a period from 1977 to 1981. HI antibody-positive sera first appeared in October, 1978. Since 1979, seropositive dogs of high HI antibody have been increasing in number. The result demonstrated that CPV was wide spread among the dogs in Japan.
Serum antibodies against bovine leukemia virus (BLV) were examined on 3, 026 cattle from 38 herds of 20 administrative districts of Iwate Prefecture. They were detected positively in 1, 322 cattle (43.7%), which consisted of 411 (38.2%) of 1, 076 dairy cattle and 911 (46.7%) of 1, 950 beef cattle, and which came from 27 herds (71.1%). The positive rate was 9.5 to 100% in each herd. It increased with the advance in age. It was more than 40% in every age group of 3 years or more, except that of 8 years. All 117 cattle affected with enzootic bovine leukosis (EBL) had serum antibodies against BLV. BLV particles were demonstrated from 60 (93.8%) of 64 cattle examined by electron microscopy. The cattle with EBL were from districts close to those highly contaminated with BLV.
The concentration of three vitamin D3 metabolites were measured in the serum of dairy cows at puerperium using ligand binding assay. The 1, 25-dihydroxy vitamin D3 (1, 25-(OH) 2-D3) serum concentration increased responsively to hypocalcemia during parturition. The serum level of phosphorus increased markedly in corresponding to the elevation of the level of 1, 25-(OH) 2-D3 in a period following parturition. The serum levels of 25-hydroxy vitamin D3 and 24, 25-dihydroxy vitamin D3, however, changed independently of serum mineral levels. These results indicate that homeostasis of calcium and phosphate during puerperium is tightly concerned with 1, 25-(OH) 2-D3.
Typical calcinosis occurred in two adult dairy cows which had been administered with vitamin D for a long time to prevent osteomalacia after delivery. The cows displayed emaciation, weakness of heart sound, distention of the jugular vein, and diarrhea. Pathologically, Severe generalized calcification was revealed in them. Main calcified lesions were in the left atrial and ventricular endocardium of the heart, the media of arteries, the adventitia of veins, trabeculae of the spleen, the basement membranes of tubules of the renal medulla, the serosa of the rumen and omasum, and the gastric glands of the abomasum. Cartilaginous metaplasia was observed in some of the affected arterial walls. In the lungs calcification was seen in the bronchial cartilage, and multiple bony metaplasia in alveolar walls. The present cases were considered to have been caused by an excessive administration of vitamin, D.