A total of 207 bitches of 24 breeds with pyometra were examined with the following results. The highest incidence, 30.3%, was found in the Spitz. The bitches ranged from 1 to 16 years old. The highest occurrence, 13.2%, was seen in the age group of 10 to 11 years. No bitches under 1 year old were affected. Regular estrous cycles were repeated in 77.7% of the bitches. The interval between the last estrus and the onset of pyometra varied from under 1 month to 4 years mostly less than a month. Nulliparous bitches were affected with pyometra most frequently. There was a tendency for the occurrence of pyometra to decline with the increase in parity. The interval between the last delivery and the onset of pyometra ranged from 20 days to 9 years with an average of 4 years. It was more than 5 years in about a half of the affected bitches. It was a characteristic finding that shortly before the onset of pyometra the highest copulation rate, 19.8%, was seen at the last estrus. The results of this survey suggested that the primary important factor involved in the pathogenesis of canine pyometra might be uterine dysfunction under the normal ovarian control, but not the ovarian abnormality.
Biochemical tests were conducted in 35 cows affected with so-called gangrenous mastitis (24 endotoxin-positive and 11 negative cows) and 12 healthy cows. The affected cows showed an increase in red blood cell count, hematocrit, and staff neutrophils and a decrease in eosinophils, segmented neutrophils, and monocytes. In addition, they revealed a decrease in total protein, albumin, globulin, and calcium and an increase in blood urea nitrogen and inorganic phosphate. A decreace in segmented neutrophils and monocytes and an increase in GOT, GPT, and LDH were more remarkable in the endotoxin-postitive cows than the negative ones. Enzymatic activity was much lower in the udder tissue of the affected cows than in that of the healthy ones. It was a little lower in the heart, liver, and kidney of the endotoxin-positive ones. These findings were similar to those of endotoxemia in human beings.
Scabietic crust was collected in a Petri dish by scratching with a sharp spoon to such extent as to make blood ooze from the skin. It was observed immediately by the microscope or observed microscopically after heated at 37°C for 30-60 minutes. Sarcoptes scabiei var. suis was detected from the crust, unheated and heated, in August, but only from the crust heated at 37°C for 60 minutes in November. In a therapeutic experiment 1: 500 dilution fo KWA-905 (containing 36% Naredo), an organic phosphorus scabicide, was sprayed on the body surface of 46 breeding sows three times at 3-day intervals. All the sows recovered completely in 38 days, except three in which the crust remained inside the auricula probably due to incomplete spraying. The same dilution was sprayed only once upon piglets, all of which recovered. Clinical and hematological examination revealed the safety of this scabicide.
A survey was conducted on the pollution of water with pyrogens by the routine pyrogen test (PT), Limulus test (LT), and general bacterial count (BC). In PT, 10 ml/kg of undiluted tap or well water was pyrogenic when injected, into rabbits. It was also positive for LT. BC was 0-103/ml for tap and well water. In PT, 1 and 10 ml/kg of undiluted river and sea water were pyrogenic when injected into rabbits. LT was positive when applied to undiluted river and sea water and dilutions up to ten times. BC was 103-106/ml for river water and 10-103/ml for sea water. Rain and snow water were negative for PT and LT even when undiluted. BC was less than 102/ml for rain water and less than 10/ml for snow water. When tap water was heated at 100°C for more than 10 minutes or at 121°C for more than 5 minutes, it became negative for PT. Tap water became negative for PT when filtered through a membrane filter 0.45μm in pore diameter or an asbestos filter plate 0.08μm in pore diameter.
Human and porcine serum samples were collected in Chiba Prefecture where outbreaks of abortion and the congenital arthrogryposis-hydranencephaly (AH) syndrome had been observed among cattle in 1972-1973. A total of 1, 348 human serum samples were collected over a period from June, 1977 to April, 1979, and a total of 1, 134 pocine serum samples (654 fattening pigs 4-5 months old and 480 sows) over a period from September, 1978 to February, 1979. A relatively low incidence of inhibition against Akabane Virus hemagglutinin (HI) was obtained with human (1.3%) and porcine (1.4%) serum samples. The HI was observed with human sera collected in the countryside of the eastern part of Chiba Prefecture, where the AH syndrome was prevalent. The positive rate of HI was 0.8% in the fattening pigs and 2.3% in the sows.
On a hog farm 68 (17%) of 411 newborn piglets began to suffer from pyrexia, mucohemorrhagic diarrhea, and dehydration over a six-day period and died within 20 hours to 5 days. Autopsy revealed hyperemia, hemorrhage, and edema in the jejunum. The stomach contained milk components. Histological charaeteristics were hemorrhage and necrosis of areas extending from the tunica propria to the tunica muscularis in the jejunum and in some part of the ileum. From clinical and pathological findings this disease was diagnosed as necrohemorrhagic jejunitis induced probably by Clostridium infection.
A 7-month-old female calf showed symptoms of leukosis, weight loss, lymphocytosis, exophthalmos, and remarkable swelling of general lymph nodes. Infiltration and accumulation of atypical lymphoid cells were observed in the lymph nodes, heart, abomasum, small intestine, uterus and brain, but not in Glisson's sheath of the liver, bone marrow, or thymus. These lesins were similar to those in leukosis of adult type. On the farm where the calf was kept, the rate of cows positive for BLV-antibody test was 28-52%. The calf had also BLV-antibody, but no BLV could be isolated from her. In brief, these lesions were similar to those of the adult cow, although they were presented by the calf 7 months of age.