Cows grazing in some pasture frequently secreted milk unstable for the alcohol test, while those grazing in another pasture did not. A survey revealed that they had been fed abundant roughage and little sodium chloride. When they were fed this mineral sufficiently, sodium increased in level in their milk to make the Na/K ratio high and they became negative for the alcohol test.
When prednisolone was administered, the cows secreted milk low in Na level and Na/K ratio and became positive for the alcohol test. When administered with membutone, they secreted milk high in those level and ratio and became negative for this test.

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Were studied bacteriologically and Ten piglets affected with E. coli diarrhea (white scours) treated with piromidic acid with the following results.
1) Diarrhea occurred in piglets 2-4 weeks old at a high incidence. It was closely related to the unusual growth of E. coli in the duodenum.
2) The viable E. coli count in the contents or on the walls of the duodenum was higher in diarrheal than in normal or healing piglets.
3) Enterotoxigenic E. coli was detected from diarrheal piglets. It had the colonization factor, K-88, and a capacity of producing a heat-stable enterotoxin.
4) Oral administration of piromidic acid was effective for the control of diarrhea. It caused healing of the duodenal lesion and the reduction in viable E. coli count there.

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Hemagglutination-inhibiting (HI) antibody titers against canine parvovirus (CPV) were determined in the sera of 12 puppies of 3 litters to know the levels of maternal antibody transferred and declined in these puppies with the lapse of time.
On the other hand, HI titers of mature dogs kept by private owners in southern Osaka Prefecture were also determined.
Several days after suckling, puppies had HI titers that averaged 50% of their dam's titers. Maternally derived CPV antibody declined exponentially with the lapse of time, showing a half-life of 10.8 days.
Of 168 mature dogs, 144 (68%) had positive HI titers (≥1: 20). Sero-positive dogs kept outdoors had a geometric mean titer of 1: 1349, and dogs kept indoors mean titer of 1: 187.
Of all the puppies raised in the region, 32% had no maternal antibody. The HI titer disappeared from sero-positive dogs kept outdoors by 72 days of age on the average and from dogs kept indoors by 42 days of age, on the average. Consequently, it was possible to predict the optimum age of puppies for an initial vaccination with inactivated CPV vaccine.

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In 1980, 49 samples of feces and 40 samples of leukocytes were collected from 2 flocks affected with an egg drop syndrome-1976 (EDS-76)-like disease in the Kyushu region for virus isolation in duck embryos and duck embryonic liver cells. As a result, heamagglutinating viruses were isolated only from 4 samples of feces.
The isolates had the same antigenicity and physicochemical properties as the JPA-1 strain of avian adenovirus, identified as EDS-76 virus in Japan. HI antibodies against the isolate (KE-80) were detected in more than 90% of the sera from the flocks.
Laying SPF hens and ducks inoculated perorally with the isolates began to show aberrant egg production 7 and 9 days after inoculation, respectively, and produced HI antibody.

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The precise morphology of adult worms, tetrathyridia and eggs ofMesocestoides paucitesticuluswas observed macroscopically and histologically. It was useful for discriminating this species fromM. lineatusandM. litteratus.

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Naled, a low toxic organophosphorus acaricide, was investigated for efficacy against Sarcoptes scabiei var. suis in vitro. Mites of this variety were obtained from cutaneous lesions of naturally infected piglets by incubating at about 37°Cfor 1 hour. Petri dishes with cross stripes on the outside of the bottom were used for the test. One milliliter of the drug solution at a given concentration, which had been prepared by dilution with acetone, was poured into each dish and dried at room temperature. Then 30 mites were put in each dish to calculate the knock down ratio (KT ratio) with the lapse of time.
As a result, this testing procedure was found to be a useful means for the examination of scabicidal effect of drugs. Naled was suggested to have a relatively rapid killing activity on this species of mites. The present study was carried out on adults, nymphs, and larvae of mites, since it was difficult to use mites of the same developmental stage because of the limited number of mites obtained.

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A primiparous infertile Holstein-Friesian cow was imported from Canada and inseminated 19 times for the past 2 years. Its ovarian function was monitored successively by measuring its serum progesterone level. After one full estrous cycle was detected, 800μgof an analog of prostaglandin F_2α (ONo-1052) was administered intramuscularly (i.m.) on day 12 of the estrous cycle. The cow was inseminated 3 days later. Insemination was repeated twice thereafter. On each occasion the cow was treated with bovine APG (Hypohorin), 400KE, or fertirelin acetate (an analog of LHRH), 200μg. No services were successful. During the last estrous cycle, LH-RH was injected on day 2 and O_No-1052 i.m. on day 17. The cow was inseminated at the induced estrus and treated with APG on day 2, when it conceived.

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