In order to make a DIC model, 15 normal dogs were injected with Escherichia coli endotoxin, or lipopolysaccharide, via a venous catheter for 10 hours. hours. The platelet count decreased drastically in dogs after endotoxin injection. It was maintained at a low level during the experiment. The fibrinogen amount also decreased after the injection. It was about half of the pre-injection amount 6 to 7 hours after the infusion. PT and APTT prolonged after the injection. FDPs increased in all the dogs 2 hours after the injection. They were more than 40μg/ml in 13 dogs. Autopsy revealed severe hemorrhage in the intestine and congestion in the liver, spleen and kidney in most cases. Degeneration of hepatocells, hemorrhage, and the formation of fibrin-like substances were detected in sections of the liver of the treated dogs stained hematoxylin and eosin and Weigert's staining.
Nine Suffolk ewes 3 to 4 years old from a certain farm in Hokkaido showed severe emaciation, general pruritus, alopecia and incoordination over a period from December, 1981, to May 1982. They died within 9 to 92 days since onset. Histopathological examination revealed severe vacuolation of nerve cells and a spongy state of gray matter of the central nervous system, especially midbrain, pons and medulla oblongata. A diasnosis of scrapie was made in the ewes. This disease had not been reported in Japan. It might have been caused by unconventional virus transmitted from sheep imported, since all the ewes affected were of the second generation of sheep imported from Canada in 1974.
Sixty-six serum samples from field cattle (field sera) and 17 samples of commercially available sera (calf and fetal bovine sera) for cell cultures were tested for inhibitory effect on the growth of infectious bursal disease virus (IBDV) by plaque assay. The average plaque reduction due to the field and calf sera was more than 90%, whereas that of the fetal bovine sera was less than 29%. The serum with high inhibitory effect did not react with IBDV antigen in the immunodiffusion test. This suggested that the effect might not be due to specific antibodies against IBDV. When the serum with inhibitory effect was fractionated by DEAE-cellulose column chromatography, it was found that the inhibitory effect was mainly associated with the IgG fraction, and relatively low in other pooled fractions. The activity was destroyed by treatment with either trypsin or ethyl ether but not with kaolin, acetone, heparin or receptor-destroying enzyme. The fluorescent antibody test showed that the serum with high inhibitory effect was attached nonspecifically to chicken embryo fibroblasts.
In order to elucidate the significance of thermostable antithiamine factor (SF factor), as one of the inducing factors of equine bracken poisoning, 2 horses were given orally with fluid extracted from dried bracken with water and heated at 100°C for 30 minutes to inactivate aneurinase. The extracted fluid was adjusted daily with dried bracken, which was 8.1 g/kg of body weight in one horse and 1.13 g/kg in another. The former horse manifested typical symptoms of bracken poisoning, including stiffness, staggering, and inability to stand for 29 days. The latter showed only temporal stiffness during an experimental period of 83 days. Administered with the fluid containing SF factor, both horses presented a marked decrease in blood thiamine concentration and daily excretion of thiamine in urine, and an increase in pyruvic and lactic acids in blood. It was concluded that bracken poisoning was caused by not only aneurinase but also SF factor, which had also an antithiamine action.
On December 12, 1981, five persons ate raw bear meat thawed from the frazen state in Hotel “M” in the urban area of Yokkaichi, Mie Prefecture. Of them, four suffered from pruritus, eruption, myalgia, and facial edema over a period from December 27, 1981, to January 10, 1982. Examination in a hospital revealed that they might have been involved in trichinosis. Inspection demonstrated the presence of Trichinella spiralis in the bear meat remaining in the hotel. A total of 413 persons were reported to have eaten the same bear meat and subjected to serological tests. As a result, 60 persons, including the four mentioned above, were positive for this parasite. The meat had been collected from a bear, Selenarctos thibetanus, shot in a mountain in Kyoto Prefecture, and supplied in a frozen state (-15°C-30°C).
Over a period from 1980 to 1983 serum samples were collected from 1, 005 dogs caught by 3 health centers in the southern part of Aomori Prefecture and examined by the indirect fluorescent antibody method. Sera positive for antibody against canine parvovirus (CPV) were found for the first time in October, 1980. They showed a sudden increase in number at the beginning of 1981. Their rate changed little over a period from 1981 to 1983. It was 46.0% on the average over this period. Sera showing a high antibody titer were mixed with those showing a low antibody titer every years. In 1983, the rate of house dogs positive for antibody against CPV was 54.0%. Antibody titer was in a range of 1: 4 to 1: 4096 in these dogs. It was in a similar range in retention dogs. Judging from the occurrence of clinical infection and autopsy findings, it was suggested that CPV might have invaded the southern part of Aomori Prefecture in the middle of August, 1980. Four strains of CPV were isolated from dogs clinically infected or subjected to autopsy.
A 9-year-old male mongrel cat presented dysuria in winter. The urinary bladder was quite distended and atonic. It was held under observation for approximately 2 years. A diagnosis of bilateral hydronephrosis was made in it radiographically and ultrasonographically. The patient died of uremia. Necropsy revealed the enlargement of the left kidney, the distension of the renal pelvis and the ureters, and the compression of the renal parenchyma. It was considered that the obstruction of urine outflow might have distended the renal pelvis and caused secondary hydronephrosis. This case was diagnosed histopathologically as chronic interstitial nephritis.