The protective effect of a commercial hog cholera live virus vaccine was tested on the infection with the three strains of hog cholera virus, Yamagata (type H), Miyazaki (type IM) and Fukushima (type B), which had been isolated from affected pigs during outbreaks of the disease in 1980.
Among pigs inoculated with the commercial vaccine and challenged with each of the isolates, there were some differences in the time required for acquiring immunity. Five days after vaccina-tion, however, all the pigs were protected against challenge with each type of the isolates.
When measured by using the 3 isolates, there was little difference in the development of virus-neutralizing antibody, except that the antibody measurable with the Yamagata strain tended to be produced slower than that demonstrable with the other two.
Infective titers of the Miyazaki and Fukushima strains propagated in pigs and cell cultures were at almost the same level, while the titer of the Yamagata strain in pigs was more than 100 times as high as that in cell cultures. Pigs inoculated with a large amount of virus (10
5 MLD) of the Yama-gata strain died about one week after inoculation, whereas pigs inoculated with 1 MLD of the same virus died at about 2 weeks of illness. In the case of the Miyazaki and Fukushima strains, death occurred in almost the same course of time, regardless of the inoculum size. In these dead animals, high virus titers were demonstrated in all organs, sera and urine samples examined, showing no significant differences in virus distribution among the 3 strains.
Histopathological investigation revealed no difference in pathological lesions among pigs ino-culated with the 3 strains. The findings observed in these animals were similar to those described in pigs inoculated with hog cholera virus strains isolated in the past.
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