Heartworms were clearly visualized on the echocardiogram as echogenic, spot-like or infrequently linear patterns within echofree cardiac cavities and pulmonary arteries. The mechanical sectoscanner appeared superior to the electronic linear array scanner in detecting heartworms harbored in the pulmonary trunk and a part of the right pulmonary artery. Both scanners, however, were valuable diagnostic means for visualizing heartworms in the right atrium, ventricle, and ventricular outflow tract, and the venae cavae.
Seventeen dairy cows suffering from ketosis and 12 from the postpartum paretic syndrome were injected intravenously into the jugular vein with 50% glucose solution when they were examined for the first time. In them, the two diseases were classified into types on the basis of chages in insulin secretion immediately after the injection. In ketosis, type I showed high insulin secretion just after the injection, type II high insulin secretion just before and after the injection, and type III low insulin secretion just aftr the injection. Type I could be subdivided into type I-1 which took a good course and tye I-2 which required a long-term treatment. The postpartum paretic syndrome could be classified into type I, which presented normal insulin secretion and symptoms of milk fever just after the injection, and type II, which exhibited low insulin secretion and Downer's syndrome just the after injection. Type II could be subdivided into type II-1, which showed low insulin secretion just after the ijection, and type-II-2, which revealed relatively low insulin secretion just after the injection.
A horse (Thoroughbred, female, year old) and a sheep (Uorriedale, male, 1 year old) raisea in the same barn with a cow suffering from ringworm infection, and three adult farm hands taking care of these animals were infected with Trichophyton verrucosum. The hands must have contracted the injection by contact with the ringworm cow. The horse and sheep might have been infected indirectly by contact with the hads or such environmental factors as hay and feeding tools.
Four strains of egg drop syndrome-1976 (EDS-76) virus isolated in Japan were examined for growth and pathogenicity in cultured cells and embryonating eggs of chicken and duck. All of them grew well in chick and duck embryo liver and duck embryo kidney cells. The infectivity and hemagglutination (HA) titer reached a peak 3-5 days after inoculation. These viruses grew better in cultured cells of duck origin than those of chick origin, except chick embryo liver cells. All the strains grew well in embryonating duck eggs when inoculated into the allantoic cavity or the yolk sac. The allantoic fluid had the highest infectivity and HA titer 5-8 days after inoculation. The strains did not grow so well in embryonating chicken eggs as in embryonating duck eggs. Death occurred to more than 30% of duck embryos inoculated with 104.5-107.5 TCID50 of these strains into the allantoic cavity, chorioallantoic membrane or yolk sac 8 or 13 days after inoculation. No chick embryos died after inoculation, except those inoculated with 107.5 TCID50 into the yolk sac.
Actinobacillus suis was isolated from various organs of 2 dead pigs. The pigs had white spots in the liver, petechiae in the kidney and a reddish tint a round lymph nodes. Multiple bacteial emboli were observed in blood vessels of many organs. They were composed of fibrin exudation and mononuclear cells. Some bacterial colonies were attached to the blood vessels, causing endophlebitis. Interstitial pneumonia, meningitis and hepatic necrosis were noticed. Two pigs were inoculated intravenously and two intramuscularly with A. suis isolated from the dead pigs. Each one of them died in 15 hours after inoculation and was found to have bacterial emboli in many tissues showing essentially the same distribution of lesions as in the 2 dead pigs.
Lymphomatotic lesions were observed in 17-day-old quail which had been inoculated intraperitoneally with heparinized blood of a lymphomatotic quail. Marek's disease (MD)-specific antigen was found in the feather tips of all the quails and MD-specific antibody in the serum of one bird. Intranuclear inclusions were present in the feather follicular epithelium of all the quails. In contact-exposed quails, MD-specific antigen was detected in the feather tips and intranuclear inclusions were in the feather follicular epithelium, but no lymphomatotic lesions were noticed in any Aissne.
Two piglets of the same litter, 40 days old, presented nervous symptoms on a farm in Tottori Prefecture in March, 1983. One of them lay down, trembled, paddled and showed the concussion of the eyeball. The other swayed the hind quarters, with the left intercarpal joints swollen. Histopathologically, one manifested pyogenic cerebrospinal meningitis, and the other the same disease of low severity complicated with purulent synovitis and bronchopneumonia. De Moor's group R streptococci were isolated predomiantly from the brains of both piglets and th e lung and the left intercarpal joints of the latter. They were sensitive to ampicillin, bacitracin, benzylpenicillin, cephaloridine, doxycycline, erythromycin, oxytetracycline, and spiramycin.
The biological activities of cell-free culture filtrates of three pathogenic strains of Vibrio commonly associated with vibriosis in fish (V. anguillarum NCMB6 and NCMB571 strains, and Vibrio sp. N7802 strain) were examined to determine their relations to the pathogenesis. Extracellular materials from culture filtrates of NCMB571 and N7802 strains separated by DEAE-cellulose chromatography followed by Sephadex G200 chromatography showed lethal toxicity for rainbow trouts and mice. Cytopathogenicity was observed in CHSE-214 chinook salmon embryo-derived cell line inoculated with culture filtrate of NCMB571 strain. NCMB6 and NCMB571 strains produced hemolysin against erythrocytes of eleven animal species, including fish. Hemolysin of NCMB571 strain was thermolabile, but toxic material was comparatively thermostable. These strains of Vibrio also produced alkaline proteolytic enzyme. On the contrary, N7802 strain was found to be a poor producer of both hemolysin and protease. These results indicated that the extracellular products, such as toxic material, hemolysin and protease, produced by Vibrio may be responsible for pathogenesis of vibriosis in fish. However, the lethal toxicity of culture filtrate of N7802 strain did not seem to be related to hemolysin or protease.