Of 118 bitches suffering from pyometra, 97 (82.2%) were found to be in the metestrous phase within 3months after the last estrus. At least one corpus luteum in both ovaries could be seen in 195 bitches (95.6%) out of 204 with spontaneous pyometra. These results suggested that there might possibly be a correlation between the occurrence of pyometra and the exsistance of corpora lutea. Progesterone concentrations in the circulating blood plasma were estimated in 48 bitches with pyometra. The amounts of progesterone receptors in the endometrium were examined in 10 bitches with naturally occurring pyometra. The former or the latter were not always higher than the counterpart of normal bitches at the metestrous stage. It was suggested that neither excessive secretion of progesterone nor hyperaffinity of the endometrium to progesterone might be a cause of canine pyometra.
An intra-articular injection of 2.5-10.0ml of 1% SPH was evaluated in dairy Cattle with acute arthritis (18 cases) or chronic osteoarthritis (8 cases). Of the former cases, 16 (88.8%) revealed a complete disappearance or improvement of lameness and no recrudescence. All the latter cases and one case (5.6%) of acute arthritis exhibited an improvement of lameness, but returned to the pretreatment condition 2weeks later. One case (5.6%) of acute arthritis showed no improvement. In synovial fluid analysis, color, turbidity, mucin content, consistency, concentration of protein and hyaluronic acid were restored to normal after SPH injection. No side effects were observed.
A combination of continuous feeding of oxytetracycline (OTC) and intermittent administration of doxycycline (DOTC) was evaluated in breeding chickens on a larger-scale test for 50weeks beginning with the day of hatching. Serological data indicated that the combination provided a satisfactory control on Mycoplasma gallisepticum (MG) infection. No MG was recovered during the test period. Birds treated with DOTC alone intermittently and nonmedicated control birds converted to be serologically MGpositive and produced MG. Egg-production data indicated that the hens treated with the combination showed 16 and 37 eggs/hen housed advantage, as compared with those intermittently treated and nonmedicated, respectively. Hatchability was higher in those treated with the combination. Those numbers were within the standard of the employed HN strain standard performance. In conclusion, the combination was judged to be safe and applicable to breeder flocks to keep them MG-free.
An upper respiratory disease characterized by the severe discharge of nasal secretions and sneezing broke out among piglets 6 to 25days old on a pig farm in Kitami, Hokkaido. A total of 135 piglets of 14 litters were affected with it, and 4 of them died. The nasal cavities of the affected piglets were filled with a large amount of grayish and viscid secretions. Histopathologically, the epithelialcells of the tubuloalveolar glands were enlarged and necrotized. Large basophilic intranuclear inclusion bodies were present in them, suggesting that the disease was inclusion body rhinitis. Porcine cytomegaloviral antigens were demonstrated in the turbinate mucosa and alveolar macrophages of the affected piglets by fluorescent antibody staining. The virus was isolated from those cells by inoculating them into alveolar macrophage cultures obtained from healthy pigs. This is the first report on the isolation of porcine cytomegalovirus from piglets with inclusion body rhinitis in Japan.
A review of literature revealed that Cryptosporidium was one of the causes of calf diarrhes in many countries. This condition occurred in calves on a farm. The animals involved were mostly around 3weeks of age and showed diarrhea with cryptosporidia, which were demonstrated on the epithelial surface of the mucous membrane of the small intestine.
In December, 1984, 7 piglets approximately 40days old showed respiratory symptoms with fever on a farm in the Matsumoto area. Of them, two died within a day. Autopsy on a dead piglet revealed pulmonary emphysema and white hepatization of the lungs. Haemophilus parasuis was isolated from the lungs, heart, and intestinal lymph node. The isolate was determined as PAGE type II by polyacrylamide gel electrophoresis. From those results of this examination the disease was diagnosed as H. parasuis infection.