The minimum effective dose and safety of flubendazole were examined in dogs with gastrointestinal nematodes. Flubendazole was given in an oral dose of 5 to 30mg/kg/day to 169 dogs consisting of 34 infected with ascarids, 60 with hookworms and 75 with whipworms. The minimum effective dose was 10mg/kg/day for ascarids. It was 10 and 15mg/kg/day for 2 consecutive days for hookworms and whipworms, respectively. In a safety test, 18 dogs were divided into 2 groups, which received an oral dose of 250 and 500mg/kg respectively. During an observation period for 7 days no significant changes were seen in any dog in behavior, appearance, hematology, serum or urine.
Ten dogs and 31 domestic cats were infected with Pharyngostomum cordatum. The infected animals were mostly suffering from chronic diarrhea. Ten dogs and 31 domestic cats were infected with Pharyngostomum cordatum. The infected animals were mostly suffering from chronic diarrhea.
Three adult quails were affected with ulcerative enteritis. Autopsy revealed ulcerative enteritis mainly in the middle part of the small intestine. Large Gram-positive rods were exhibited in the ulcer. Clostridium perfringens type A and Escherichia coli were isolated at each 106-8CFU/g from the contents of the ulcerative small intestine. Four mice were dead when inoculated intravenously with the suspension of this intestine. Another 4 mice were live when inoculated intravenously with the same suspension with the addition of anti-C. perfringens type A serum.
The formation of intranuclear inclusion body (IB) in feline lung cell line cells by mink enteritis virus (MEV) was inhibited nonspecifically by bovine serum. The mechanism of the inhibition of IB and the nature of the inhibitor were examined. Commercially available calf serum (CS) inhibited the formation of IB remarkably, but the inhibitory activity of fetal calf serum (FCS) was weak. The inhibitory activity of CS decreased remarkably after treatment with one of ethyl ether, trypsin, receptor-destroying enzyme, and potassium periodate, but not with kaolin, acetone, or heparin. The inhibitory activity of CS was observed in the IgG and other pooled fractions. In FCS it was observed in the IgG fraction only. The inhibitor was absorbed by normal tissue culture cells, but did not combine with viral particles. Therefore, it was deduced that the inhibitor attached itself to the surface of the cell, thereby preventing the penetration of the virus. This inhibitor was considered to be different from those which nonspecifically inhibited the hemagglutination of MEV, and which were persent in both CS and FCS.
Examinations were carried out on the extent of infections and successive countermeasures of cleaning on a farm where pigs were suffereing from mycobacterial infections at a high ratiol. 1. The rate of condemnation of the intestine due to mycobacterial infections was 8.6 to 52.7% per month, or 24.6%(182 of 741 pigs) in a slaughterhouse over a period from February to October, 1982. 2. Mybcoacteria were isolated from various lymph nodes, including the tonsil, and sawdust at a ratio of 47.1%(8/17 pigs) and 88.9%(8/9 samples), respectively. Those of porcine origin belonged to Mycobacterium intracellulare, and those of origin to this and other species. 3. The ratio of positive reaction to avian-type tuberculin was 37.9%(272/718 swine) on the farm until August. The reaction changed to negative in September. 4. The farm could completely be cleaned up in about 8 months by countermeasures, consisting of exposure and condemnation of those positive for tuberulination, environmental control and disinfection of the farm.
This technique enabled to collect porcine blood easily and safely from the coccygeal vein, without an assistant to control and restrain a pig. Pigs suffered from little stress. It was more useful than the conventional techniques to collect blood from the auricular vein and the anterior vena cava.