A 4-year-old female dachshund was first presented in July, 1981 with paraplegia. A diagnosis of hernia of the intervertebral disc was made. Treatment was done by microwave (70 W/cm2, 10 min.) and electroacupunture (the disorder place on the back, 2-3 HZ, 5 V, 10 min.) every day. She was recovered 7 days after the beginning of the therapy. A 5-year-old male cocker spaniel was first admitted in June, 1982. Chief complaints were abasia and urinary retention. A diagnosis of disorder of the intervertebral disc was made. Treatment was done eiectroacupuncture. Needles were put into the back and posterior paws to stimulate at about 2 HZ-100 HZ, 3-5 V for 12 min. He could pass urine 3 days and walk 18 days after the beginning of the therapy.
In processing plants examined, the total condemnation rate of chicken meat was 1% on the average. Diseases found were Marek's disease, enteritis, hepatitis and arthritis. Campylobacter jejuni, Staphylococcus aureus and Clostridium perfringens were isolated from the muscle with skin and from the cecal contents. Salmonella was not isolated. Their isolation rates were 60%, 45% and 60%, respectively, from the muscle with skin, and 60%, 15% and 10% from the cecal contents. Campylobacter jejuni and Salmonella were isolated from cut meat at a rate of 20% and 17%, respectively. Antibiotic or chemotherapeutic residues were not detected from the muscle with skin.
An attempt was made to isolate bovine respiratory syncytial virus (BRSV) from nasal secretions of 18 effected cattle in two herds by using cultures of Vero cells (VC) and bovine kidney cells (BK). The virus was isolated from 11 cattle (61%) in VC and 3 cattle (3%) in BK. CPE was clearey in VC than in BK. Specific fluorescent cells against BRSV were observed in VC 24 hours after inoculation. They were noticed in 10 cattle (55%) 4 days after inoculation.
The antibody response to Fasciola sp. was studied in 13 rabbits orally infected with metacercariae, by using double immunodiffusion reaction (DID) and counterelectrophoresis (CEP). The antigen used was phosphate-buffered saline extracts from adult flukes of Fasciola sp. Crude antigen formed 1-4 precipitation lines in the DID with infected serum. CEP gave one line with it. Precipitins were first detected in the 2nd to the 5th week after administration with metacercariae. Precipitation response could be detected in serum from rabbits with one fluke. CEP was superior to DID when performed in the early stage of infection, but was less sensitive than DID in the late stage of infection. It seemed that the two assays might be useful for the detection of Fasciola sp. infection before the appearance of fluke eggs in feces.
Samples were collected from water where pet turtles had been raised in three surveys conducted over a three-year period. Of 88 samples collected, 43 (48.9%) were positive for Salmonella. The water inhabited by green turtles showed the highest positive rate (69.2%). For simple disinfection of Salmonella in water used for raising turtles, it is effective to mix the water with 1: 2, 000 dilution of sodium hypochlorite or 0.05% benzalkonium chloride solution and allow the mixture to stand for an hour. It is desirable to discard the water after this treatment of disinfection.
A 5-year-old Holstein cow which had previously given birth to 3 normal calves was slaughtered because of prolonged gestation. It contained a fetus characterized by the absence of the head. The fetus had the rudimentary cranium, which contained the nervous tissue consisting of the medulla oblongata alone. Cranioschisis was not detected. Malformations in the urogenital organs, reduction in number of thoracic vertebrae and ribs, abnormality of shape and ossification in the sternum and atresia ani were also seen. This is a severe case of anencephaly.