The first case was found in a Shih Tzu dog 6years and 7 months old with abdominal dilatation and disturbances in urination and defecation. It revealed hypoplasia or agenesis of the vagina at the vestibulo-vaginal junction, a prominent expansion of the vagina containing a large amount of fluid and a mild degree of cystic endometrial hyperplasia of the uterus by laparotomy. From these findings, this case was diagnosed as vaginal dilatation due to atresia vaginalis with hypoplasia of the vestibulo-vaginal junction or caudal vaginal agenesis in gynatresia congenitalis. The second case was a nulliparous mongrel bith 6 years and 3 months old which had undergone ovariohysterectomy in immature day. She demonstrated similar clinical signs to those of the first case. Exploratory laparotomy revealed two large lumps occupying almost the entire abdominal cavity, the remaining left ovary, and the persisting whole hymen without both uterine horns. From these results, the same diagnosis was made as in the first case. It was presumed that estrous secretions from the reserved left ovary mignt have accumulated in the vagina and the remaining portion of the uterine body. Canine vaginal dilatation due to gynatresia congenitalis is rare. Few reports on it can be seen especially in Japan.
Many worm-cysts were found in 6-month-old Landrace hog. They were light yellow in color and from a pea to an alomnd in size. They were situated on the serous membranes ofvisceral organs, especially of the liver. The Cyst-wall was composed of two layers. The outer layer was made of cuticula and the inner of a coarse reticular structure. The protoscolex of the larval cestode was provided with about 15 larger and 15 smaller hooks and four suckers. The larger and the smaller rostellar hooks were arranged alternately. The worm-cysts were indetified as Cysticercus tenuicolis from the characteristics, such as the form and nabitat of worm-cysts and the form, size, and number of rostellar hooks.
From May to September, 1984, proliferative haemorrhagic enteropathy (PHE) broke out among hogs and young breeding pigs on three closed farms in Niigata prefecture. A young breeding pig and one hog died. They were submitted to pathological and bacteriological examination, as well as three disaeased hogs. Campylobacter hyointestinalis (CHI) was isolated from the ileal mucosa of the five pigs. Viable bacterial count per gram of affected mucosa ranged from 106.4 to 107.4. Strains of CHI isolated from three farms were serologically homogenous, and were sensitive to benzylpenicillin, aminobenzylpenicillin, tetracycline, oxytetracycline, colistin, furazolidon and carbadox. Inspection was made for the occurrence of PHE on other 24 farms in Niigata Prefecture. As a result, PHE was found in 40 pigs (0.22%) of 12 farms over a period from June, 1982 to September, 1984.
Biting midges of the genus Culicoides were collected in a cowshed in Kagoshima City over a period from June to December, 1984. They were fed for 3-4 days and classified. An attempt was made to isolate virus from them by the intracerebral inoculation method with suckling mice. 1. The midges collected were classified into 13 species of Culicoides: actoni, arakawae, brevitarsis, jacobsoni, kibunensis, lungchiensis, matsuzawae, maculatus, ohmorii, oxystoma, punctatus, sumatorae and wadai. The five species marked with an asterisk were predonimant. C. oxystoma was the most predominant. 2. Three strains of Akabane virus were first isolated from materials in which C. oxystoma had been mixed with C. jacobsoni, C. muclatus, or C. punctatus collected on October 12 and 15 and Novemler 4. 3. A survey on antibody against arbovirus was conducted in two sentinel calves. Neutralizing antibody turned to be positive against Aino virus in one calf on October 1 and 15 and against Akabane virus in the two calves on November 1. No neutralizing antibodies turned to be positive against Bluetongue virus type 1, Bovine ephemeral fever virus, Getah virus, Ibaraki virus, or Japanese encephalitis virus in either calf.
The newly combined disinfectant, VEK-100-124, which consisted of 88.5% orthodichlorobenzene, 1.5% quinomethionate, and 10.0% of an emulsifier, was examined for effect against Eimeria tenella oocysts and broiler farms. Sensitization with 1: 400 solution for 10 minutes inhibited sporulation of unsporulated oocysts completely and that with 1: 500 sloution for 10 minutes sporulation of 97.4% of such oocysts. When the new drug was mixed with 5% feces, its disinfectant effect did not fell at its 1: 500 solution. Sporulated oocysts sensitized with 1: 100 solution for 24 hours were adminiter to chickens, in which the oocyst count of the ceca was lower than in control chickens. On broiler farms, no oocysts could be detected from disinfected buckets until shipping to a poultry slaughterhouse. No adverse effects were found on growth, living body weight, or feed conversion.
A field trial was done on a combination of oxytetracycline (continuous feeding at 250g/ton of feed over a period from to 60 weeks of age) and doxycycline (intermittent 5-day-administration, 15 times until 60 weeks of age) in 20 flocks of 11 layer breeder farms (in 8 prefectures) and 19 flocks of 13 broiler breeder farms (in 9 prefectures). The 24 farms had been suffering from Mycoplasma gallisepticum (MG) and M. synoviae (MS) infection and treated mainly with tylosin or other macrolide antibiotics. The combination program was compared with those conventional programs of each 39 flocks as controls on the results of serum inspection and performance data. The combination kept 37 flocks (95%) MG- and MS-free, but only 2 flocks (5%) were kept in the controle unitl 60 weeks of age. It showed a better performance in terms of hen housed eggproduction which was 8.4 and 10 eggs in layer- and broiler-breeder flocks, respectively, over the conventional programs. Hatchability was also improved 1.5% and 1.8% in the respective flocks without any side effects. Those perfbrmances were as good as those of the standards of each strain. In conclusion, the combination was applicable safely and effectively to the field condition for the prevention of Mycoplasma infection.
Calf-type bovine leukosis developed in female twin Holstein-Freisian calves. The markings of the hair coat were not similar. Clinically, both calves showed swelling of body surface lymphnodes, fervescence, emaciation, and diarrhea. A large number of immature lymphocytes appearted in the peripheral blood. The animals died at 155 days (No.1) and 199 days of age (No.2). Enlargement was seen in the following lymphatic organs: the thymus, lymphnodes of the body surface, endothoracic and intracelial lymphnodes, spleen, and kidneys as well. Microscopically, immature neoplastic lymphocytes infiltrated the lymphnodes, liver, spleen, kidneys, lung, and bone marrow. In praticular, the neoplastic lymphocytes infiltrated the Glisson's sheeths of the liver. As reported previously this seemed to be one of the distinctive features of calf-type Bovine Leukosis. In addition, Bovine Leukosis Virus (BLV) antigens were not detected in their serum.