The liver therapeutic, thiopronine (2-mercaptopropionylglycine, MPG), was administered to ketotic cows. When 50-100mlof NPG was initravenously administered alone or in combination with some other therapeutics to these cows, recovery rate was 96.0% and 88.6%, respectively.
The result of the dose response test indicated that the optimum volume of MPG to a ketotic cow was 50 or 100mlat a time, which was given five times or less. The therapeutic effect of MPG types of ketosis caws, was lower on the nervous type than on the digestive or concomitant types.
In ketotic cows in which prognosis was good, there was a significant decreases a (p<0.05) in SGOT, CPK, NEFA, BUN, and A/G, and a significant increase (p<0.05) in TP, Ca, and Glu. In cows in which prognosis was poor, there was no improvement in any parameter and the Ca level characteristically decreased. MPG tretment seemed to induce precipitation of cellular metabolism, activation of enzyme activity of carbonate metabolism, and acceleration of lipid and protein metabolism of the liver cells.

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High performance liquid chromatography (HPLC) was used to measure serumβ-carotene in dairy cows. When a Zorbax-ODS column, which is a non-aqueous reversed-phase (NARP) column, was used, a reliable routine method was assumed to be one with a methanol/chloroform (85/15) solution was used as a mobile phase.
When estimated by this method through out a year, serumβ-carotene levels of 8 cows fed sufficient roughage were about 350μg/dlin winter and about 600μg/dlin summer. This difference in level was considered to depend on the amount of green grass fed to the cows.

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The volume and nature of pus in the uterine cavity of bitches with pyometra were examined. The relationships between them and bacterial species isolated were discussed.
1. The volume of pus was 266.7±374.9 mlon the average per 10kg of body weight. It was less than 100ml in 51.2% of all the bitches.
2. The pus was classified into three color groups, reddish brown, yellowish green and milky white. The first one was largest (78.6%).
3. The viscosity of pus varied from watery to pasty. It was thick-soup-like in 28.3% of the bitch, being the most frequent.
4. In bitches from which Gram-negative bacilli, includingEscherichia coil, were isolated the volume of pus was larger and its viscosity higher than in those from which Gram-positive bacteria were isolated.

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A survey was conducted on the water pollution of rain and hot spring by examining pyrogenic contents and bacterial counts.
Samples were anaylzed for pyrogenic contents by the Japanese pyrogen test (PT) and Limulus test (LT) and for total colonies (TC) and coliforma (CF).
Rain water was negative for PT, LT and CF, and less than 300/mlfor TC. Hot spring water was negative for CF and less than 100/mlfor TC. When it was blended with other types of water (tap water, lake water, etc.), the blend was positive for PT and LT, and had a small quantity of pyrogenic contents. A similar blend of hot spring water was negative for these tests.

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An epizootiological survey on hemagglutination inhibiting (HI) antibodies to equine influenza viruses (EIV) was carried out with horse sera collected from several districts in Kumamoto Prefecture over a period from September to October, 1984.
Most of the sera of 600 racehorses with a history of EIV vaccination showed a high HI- antibody titer, a titer of 1: 8 or more being regarded as antibody-positive. Then 63.2 to 71.2% of the sera with a history of the vaccination showed an HI- antibody titer when the N. T. and P strains of EIV were used as antigens. The positive rate decreased to 5.5 to 24.7% when the M. F. S. and K strains of EIV were used. The positive rate among 106 sera with no history of the vaccination was about a half of that in the former group.
HI antibody was not detected from the sers of 123 meat horses, except 3 which showed a titer of 8 to 16 to the N and T strains of EIV. It was presumed that the meat-horse group might have not been exposed to EIV infection.It should be noted that HI antibody to the N strain of EIV (subtype 1) was detected in most of the serasurveyed.

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An outbreak of fibrinous pleuropneumonia was observed on a farm in Nagasaki Prefecture from September to October, 1982.
Sudden death occurred mainly in four-month-old fattening pigs. Autopsy on two dead pigs revealed hepatization in all the lung, fibrination in the pleura, and deposition of fluid in the thoracic cavity. Histologically, fibrinous pleuropneumomia was demonstrated.Haemophilus pleuropneumoniae, which belongs to serotype 5, was isolated from the lung and heart. Of 57 strains isolated in Nagasaki Prefecture from 1982 to 1985, six belonged to serotype 5 and the others to serotype 2.

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Serological and microbiological surveys were performed on Specific pathogen-free (SPF) chicken flbcks, establishedin1979, on the Yakumo farm, Kitasato University. Atotalnumberof30, 445 chickens were examined during a six-year period ending in April, 1985. All of them were free from antibodies against avian infectious bronchitis, avian encephalomyelitis, avian infectious laryngotracheitis (ILT), avian reticuloendotheliosis, Marek's disease, infectious bursal disease, avian viral tenosynovities, avian adeno infection (AAV), avian leukosis and sarcoma (subgroups A and B), Newcastle disease, avian influenza, avian parainfluenza, the egg drop syndrome-1976, infectious coryza, pullorum disease (SP) and mycoplasmosis of chickens (M. gallisepticum, MG;M. synoviae, MS).
A few of them, however, presented nonspecific reactions to SP, MG, ILT and AAV antigen.Escherichia coli, Proteus spp. andStaphylococcusspp. were isolated from intestinal samples as normal flora, but no mycoplasmas or viruses were isolated.
These findings indicated that the SPF chicken flocks had been maintained in the pathogen-free condition.

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A comparison was made between enzyme-linked immunosorbent assay (ELISA) and indirect fluorescent antibody technique (IFA) for the detection of antibody in the serum of cattle infected withBabesia ovata.
1. Antigens were prepared from intraerythrocytic piroplasmas in the early stage of infection. Optical density (OD) of positive serum was more than 0.199. A significant difference (P<0.01) was recognized in OD between positive and negative serum by IFA.
2. In calves experimentally infected withB. ovata, the antibody titer was detected by ELISA and IFA 10 days after inoculation and reached a peak in one month to decrease gradually thereafter.
3. Changes in positive rate of serum antibody were observed by means of ELISA and IFA in cattle contaminated with vector ticks ofB. ovatain three pastures. The positive rate reached about 100% in 2-3 months by both assays. In the early stage of infection, however, ELISA showed a higher average of positive rate than IFA.
In conclusion, ELISA was considered to be a useful method for the serodiagnosis ofB. ovatainfection.

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In April, 1985, a Holstein cow approximately 3 years old died on a farm in the Matsumoto area after exhibiting chronic pneumonialike symptoms and finally hemoptysis.
Autopsy revealed a goose-egg-sized thrombus in the thoracic caudal vena cava. Multiple abscesses and lobular hemorrhages were seen in the lungs. Histopathologically, the thrombus was replaced by granulation tisssue and attached to the wall of the caudal vena cava. A large quantity ofFusobacterium necrophorumwas isolated purely from the thrombus and pulmonary absecsses.

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Verrucous endocarditis was found in a comparatively rare position of the left ventricle in an emaciated two-month-old pig at a slaughterhouse.Haempohilus pleuropneumoniaewas isolated purely from endocarditic warts, myocardium and kidneys of the pig.

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