Changes in ECG by alteration of serum Ca concentration were observed in cattle. Eleven parturient paretic cows and six experimentally-induced hypocalcemic calves were used for this experiment. They were treated with Ca borogluconate. As a result, significantly prolonged QoTc duration and ST segment, and flattened diphasic and sometimes inversed T wave were recognized in the cattle with hypocalcemia. There was a significant correlation between serum Ca concentration and QoTc or QTc duration in these cattle.
A total of 33 bitches with experimentally induced pyometra were examined. When the uterus was in the stage from proestrus to diestrus, it was heavier than that in any other stages. It was smaller than the uterus spontaneously affected with pyometra. The uterus did not show characteristic shapes depending on a given in the estrous cycle. It was most frequently of segmental shape in the stage from proestrus to diestrus and pear-shaped in the postpartum stage. Cystic endometrial hyperplasia which ordinarily could be seen in spontaneous disease was reproduced in only one bitch that was in the diestrous stage.
A female cat 9 years old showed anorexia, vomiting, splenomegaly and a high-grade appearance of mast cells in blood. Pathologically, she presented a marked proliferation of mast cells in the liver and spleen, and ulcerations of the duodenum. A diagnosis of mast cell leukemia was made in this cat.
In sera of pigs in which so-called “liver degeneration” was noticed after slaughter, considerable increases in GOT, GPT, LDH (especially LDH-2 and -3) activities and NEFA were observed. In their livers, a very low level of glycogen, a decrease in acetone-methanol extract and remarkable increases in lipids (NEFA and TG) were found. These changes were rather remarkable in pigs forced to be starved for a rather long period before slaughter.
Over a 2-year period from January, 1984 to December, 1985, there were 46 bovine cases of abnormal births. Of these cases, 10 were abortion, 11 stillbirths, and 25 abnormal newborn calves with kyphosis, blindness, infirmity, hydrocephalia and cerebellar hypoplasia. All the calves had cerebellar encephalitis. Seven stillborn young had neutralizing antibody to Ibaraki virus, but not antibodies to Akabane virus or bovine viral diarrhea-mucosal disease virus. It was suggest that these cases of abnormal births might have been caused by infection with Ibaraki virus or a virus which had the same antigen as this virus.
Pigs were challenged with oocysts or tachyzoites of the Toxoplasma (Tp) SS strain after inoculation with formalin inactivated Tp antigen with Freund's complete adjuvant. In the case of challenge with oocysts, all the inoculated pigs survived, but uninoculated pigs died within 11 or 15 days after challenge, so that the evident protective effects were shown. In the case of challenge with tachyzoites, both groups of pigs survived, but the inoculated pigs were in a shorter febrile condition than the uninoculated. After the challenge, latex agglutination titer increased rapidly, and parasitemia and Tp cysts were observed in the brain and muscle in all the survived pigs.
A 2-month-old piglet was orally inoculated with 1.7×106 sporocysts of Sarcocystis miescheriana which had been collected form canine feces and stored at 4°C for about 1 month. In it, temperature rose rapidly to reach 41 to 42°C 11 days after inoculation. Pulse and respiratory rates increased and appetite was lost in the febrile stage. The piglet died 12 days after inoculation. A large number of petechiae were found in almost all the organs. The histopathological changes observed were hemorrhages and infiltration by mononuclear cells in the whole body. Schizonts were present in the small blood vessels of 24 of 30 kinds of tissues, being 16.7×9.3μm in average size. Japanese S. miescheriana was found to be highly pathogenic to swine.
Forty 30-day-old ducklings died in a flock of 400 birds on a farm with 2, 900 mest ducks in Octover, 1985. They showed nasal and ocular discharge, tremor of the neck, ataxia, and diarrhea. Auropsy on 5 ducklings revealed gross lesions consisting of hepatomegaly, splenomegaly with light gray mottling, spots and fibrionus pericarditis, and edema in the brain. Histopathologically, cell infiltration was seen in heart, lung, liver, kidney, and spleen meningoenchepalitis in the brain and epicarditis. Pasteurella anatipestifer was isolated from the brain of 5 ducklings and the lung, liver, kindney, and spleen of 1 bird. It was sensitive to PC, AB-PC, SM, TC, CM, CL, and EM and resistant to PMB, SDM, and SMM. The diease was diagnosed as Pasturella anatipestifer infection.
During a period of 1979 to 1985, a total of 280 Mycoplasma gallisepticum (MG) strains were isolated from 228 diseased layer chickens housed on 51 commercial farms in varying geographical areas of Japan. Of 55 chicken flocks investigated, 45 showed respiratory symptoms and 10 were affected with synovitis. The 45 flocks consisted of 11 flocks of 50 growing chickens and 34 flocks of 131 adult chickens. They were characteized by nasal discharge, catarrhal exudate in the trachea, and airsacculitis, but never synovitis. Facial edema was also observed in adult chickens mainly in the case of mixed infections with Haemophilus paragallinarum. The chickens of this group had a high level of hemoagglutination inhibiting titer against MG. The organism was isolated frequently from the nasal cavity and trachea, and occasionally from the air sacs, but never from any joint. The 10 flocks with synovitis consisted of in 8 flocks of 36 growing chickens and 2 flocks of 11 adult chickens. They were characterized by swelling of the hock joint and/or breast blisters, and few of them showed respiratory symptoms. In them, MG was frequently isolated from hock joint, breast blisters, nasal cavity, and tracheas.
A male dog of the AKITA breed, 3 years old, weighing 18 kg, showed scleroderma-like skin lesions all over the body. Mask-like expression of the face, difficulty in blinking and mouth opening, and stretching gait were markedly observed. Appetite and energy were substantially normal. It was not so difficult to take enough meal when he spent much time. Skin biopsy showed predominant hyperplasia of collagen fibers and atrophy of sweatand sebaceous glands. Anti-nucleus antibody and C-reactive protein (CRP) test were negative. Thorn's test to evaluate an adrenocortical function was indicative of mild dysfunction with an eosinophile reduction rate of 56.5%. The dog died suddenly of torsion of the intestine and peritonitis on day 104 of observation.