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Volume 41, Issue 8
Displaying 1-11 of 11 articles from this issue
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HIROSHI KAMATA, YOSHINORI YANAI, KAZUO IMAIZUMI, YOICHI FUKUDA, EIJI M ...1988 Volume 41 Issue 8 Pages 549-554
Published: August 20, 1988
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSNormal flora in the canine oral cavity was studied using 30 male and 20 female dogs having no periodontics. For the primary isolation, nine different kinds of selective media for 1 family and 8 genera of bacteria were used. The dogs revealed a high incidence of Staphylococcus (80.0%), Streptococcus (74.0%) and Fusobacterium (66.0%). Bacteroides and Enterobacteriaceae (Escherichia, Proteus, Klebsiella) were also isolated. Above mentioned four genera were found to be common in 47 dogs. In these cases, the isolation rate of cocci was rather low and that of obligatory anaerobic rods was rather high. The recovery rate of Veillonella (26.0%) was similar to that of Lactobacillus (22.0%). Candida (14.0%) and Pasteurella (10.0%) were isolated less frequently. The recovery rate of Pasteurella was almost the same as previously reported.
Approximately 90% of the isolates except Candida strains, showed sensitivity against Chloramphenicol and Aminobenzylpenicillin. On an average, 85.3% of the isolates exhibited sensitivities to penicillin/cepharosupolin. The isolates also showed a 75% sensitivity to macrolide and 71.5% to aminoglycoside analogs. The average percentage of sensitive strains of Enterobacteriaceae to antibiotics revealed the lowest average sensitivity rate of 28.7%, except for Naridixic acid.View full abstractDownload PDF (814K) -
YUKIO OKADA, TADASHI TAKUWA, FUMIO HARA, TADAAKI SHIRAISHI, SHINOBU FU ...1988 Volume 41 Issue 8 Pages 555-560
Published: August 20, 1988
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSNine Japanese Black bulls diagnosed as having fat necrosis were kept in the Shimane Prefectural Livestock Breeding Center and given daily 7.5-30 g of a soybean oil extraction by-product; Soysterol, which contains fatty acids and several kinds of plant sterol. Thy were then observed for 20 months.
Rectal examination showed that the mass of fat necrosis around rectum and kidneys was softened after 1 to 2 months. It reduced in size after 4 to 6 months, and disappeared after 6 to 10 months. However, the mass of fat necrosis around the colon did not disappear even after 20 months, although softening and reduction were observed.
Three cases showed serious clinical signs such as bloody diarrhea and anorexia. The treatment with Soysterol was not effective, and all of them were slaughtered.
Hemobiochemical examination showed a decreasing of GOT, γ-GPT and inorganic phosphorus and an increasing of magnesium after 10 months treatment.View full abstractDownload PDF (4547K) -
YUTAKA OONUMA, SHIGERU MIZOI, TOSHIRO ISHIYAMA, SHUJI HONMA1988 Volume 41 Issue 8 Pages 561-564
Published: August 20, 1988
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSFrom 1984 to 1986, in Tokachi District of Hokkaido, a total of 34 cases of Salmonella typhimurium infection among male calves on 21 dairy farms were reported.
The plasmid profiles of 36 strains isolated from the calves were examined. The strains were identified by plasmid profile as follows: 20 strains were Type A, 10 strains were Type B, 3 strains were Type C and 1 strain was Type D. There were 2 unknown types.
In the latter half of 1984, Salmonella typhimurium strains with plasmid profile of Type B were isolated on 6 out of 9 farms. On the other hand, strains with plasmid profile of Type A were isolated. on 9 of 11 farms in the latter half of 1985. These facts indicated that the plasmid profile of the predominant strains was changed from Type B to Type A in this district. There also exists the possibility that the organism with the different plasmid profile invaded a farm via new calves.View full abstractDownload PDF (2735K) -
SHIGERU ICHIJO, SATOSHI OSAME, TAKAO TAKEDA1988 Volume 41 Issue 8 Pages 565-570
Published: August 20, 1988
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSSelenium and tocopherol levels in the blood of female beef cattle (3 to 7 years old), feed and soil of grassland were determined at an early (November, 1985) and end stage (May, 1986) of housing on 21 farms in Hokkaido.
The average levels of serum selenium and blood glutathione preoxidase (GSH-Px) activity of the beef cattle showed deficient values lower than 35 ppb in selenium and 30 enzyme units in GSH-Px activity in all the farms at the early stage and in 71.4% of the farms at the end stage of housing.
The selenium level in the feed and hay showed a lower value than 0.05 ppm in 25 samples out of 42 (59.5%) at both the early and end stages of housing. In addition 10 samples out of 20 (50%) of other roughages including grass silage, haylage, rice and wheat straws examined, revealed low levels of selenium under 0.05 ppm. Upon examing the selenium level in the soil of the grassland, water soluble selenium, which is available for the plant, was lower than 0.1 ppm and the selenium level of the grass was also at a very low level.
The α-tocopherol level in the feed, hay was lower than 3mg/100g in 13 samples out of 18 (72.2%) at the end stage of housing. However the other roughages and concentrates also showed low levels. Accordingly, serum tocopherol of the beef cattle at the end stage of housing showed a lower level than 200μg/100ml in 5 out of 21 farms (23.3%).
From the results described above, it was observed that if beef cattle in Hokkaido are mainly fed with roughage, it might cause probable marked low levels in both serum selenium throughout the year and of serum tocopherol at the end stage of housing.View full abstractDownload PDF (735K) -
KAZUHIRO AIMI, SHIGEKATSU MOTOYOSHI, SHIGENORI IKEMOTO1988 Volume 41 Issue 8 Pages 575-578
Published: August 20, 1988
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSThis paper dealt biochemically with the analyzation of the agglutinin structures of Feijao chumbinho (Fc) lectin. The agglutinin activities were observed on effluent obtained by applying a gel filtration with Sephadex G-50, G-100 and G-200 on the third partition prepared by purifying the extract of albumen in Fc seeds by an ethanol method. These effluents and Fc agglutinin were subjected to immunoelectrophoresis to observe 4 precipitation lines for immune antigen and 2 precipitation lines for the effluents by Sephadex-G 200. The agglutinin activity was recognized and observed in its component. The precipitation coefficient was measured by the SDS gel electrophoresis. The molecular weight approximately 160, 000 was obtained. Fc agglutinin was inhibited by Dglucose, D-mannose and L-ribose.View full abstractDownload PDF (2241K) -
SATOSHI ONEDA, YOSHIO KIMURA, MITSUO SATHO, MITSUO SAITHO, TORU INOUE1988 Volume 41 Issue 8 Pages 579-582
Published: August 20, 1988
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSA calf of two weeks old showed purple spots and hemorrhages in several parts of the skin. At necropsy, petechiae and ecchymosis were observed in the skin, subcutaneous tissue and serosal surface of visceral organs. Hematologically, no thrombocytes could be detected in blood smears and clot retraction did not occur. Light microscopy revealed multiple hemorrhages in the subcutis, submucosa and serosa of the intestinal tract, and in the other internal organs. In the bone marrow, megakaryocytes appeared normal but slightly increased in number, whereas thrombocytes were decreased.
The number of thrombocytes markedly decreased, when blood samples from normal calves were mixed with blood plasma of the diseased calf or when the rabbits were injected intravenously with the serum from the diseased calf. In the former case, aggregates of thrombocytes were frequently seen. It suggested the presence of a platelet agglutinating factor in the patient plasma.
Based on the data collected, the present case was diagnosed as thrombocytopenic purpura.View full abstractDownload PDF (5169K) -
YOUKO KIRIHARA, JUNICHI YONEDA, YUKIO YOSIDA, MITUO KITA, TAMAKI KUBO1988 Volume 41 Issue 8 Pages 583-586
Published: August 20, 1988
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSA dairy cow was suffering from a fever and cardiac murmur for 3 weeks. At autopsy, the formation of a verruca was observed on the valva atrioventricularis dextra. The verruca was the size of an egg with a shape resembling that of a cauliflower. Cardiobactrium hominis was isolated in pure culture from the verruca. Therefore, the present case was diagnosed as having endocarditis verrucosa. The present case may be the first diagnosis of bovine endocarditis verrucosa caused by Cardiobacterium hominis.View full abstractDownload PDF (2412K) -
AKIHIRO WATARI, MASUO INOUE, KIYOSHI SAIDA, OSAMU KURIHARA, YASUO NOMU ...1988 Volume 41 Issue 8 Pages 587-589
Published: August 20, 1988
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSA 5 years old sow, weighing 200kg, developed a lymphosarcoma on the pericardium. The tumor masses were yellowish and had diameters ranging from 10 to 30mm. They were scattered on the pericardium. Thus, the masses were not found in other organs. Microscopically, the tumors consisted of lymphocytes. The tumors were covered with a single layer of mesothelial cells. The tumor cells infiltrated into the myocardium in the coronary sulcus.View full abstractDownload PDF (5645K) -
NAOYA YOSHINAGA, NAOKI ENAGA, HIROAKI ICHIMARU, TOYOICHIRO MURAOKA, HI ...1988 Volume 41 Issue 8 Pages 590-593
Published: August 20, 1988
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSIn September of 1986, on a swine breeding farm in Saga Prefacture, three-33-day-old, weanling piglets were stricken with diarrhea. As a result, two piglets died and one was in a moribund state immediately after the onset of diarrhea.
Histological examination confirmed numerous round protozoa in the epithelial cells lining the crypts of the cecum and the colon of the dead and moribund piglets. The protozoa were 1.5 to 5μm in diameter and were PAS positive. Electron microscopic examination revealed the protozoa in various stages of the development and the disappearance of microvilli at the affected colon. Characteristic findings observed in the present cases corresponded to those previously reported on Cryptosporidiosis.
Due to the findings of catarrhal enteritis in the non-infected small intestine, marked demyelination of the central nervous system of the moribund piglets and OPG obtained in the other healthy piglets of the same litter, some factor other than cryptosporidiosis also be involved.View full abstractDownload PDF (3188K) -
HARUO KOBAYASHI, AKIRA YUYAMA1988 Volume 41 Issue 8 Pages 594-597
Published: August 20, 1988
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSDownload PDF (595K) -
YOSHIO TANAKA1988 Volume 41 Issue 8 Pages 603-606
Published: August 20, 1988
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSDownload PDF (826K)
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