Three methods, such as eyedropping, suboculoconjunctival injection and subblepharoconjuntival injection, were tested comparatively as the therapies for pinkeye infectious keratoconjunctivitis. The method of eyedropping needed maximum 20 days treatment and 3 therapeutic drugs. The suboculoconjunctival and subblepharoconjunctival injections healed the pinkeye with only one shot in the mild and medium cases. Severe cases of pinkeye associated with purulent keratitis, keratic ulcer and blepharoconjunctival ulcer were healed with 3 injections on alternating days. From these results, subblepharoconjunctival or oculoconjunctival injections were proven to be the most suitable therapy for pinkeye.
Seven Holstein dairy calves suffering from abomasal volvulus were studied. All were presented in serious physical condition due to the acute course of the disease. Dehydration, depression, abdominal distention and “ping” sound were the common clinical signs in these seven calves, of which six showed hypochloremia and four were in lactic acidosis. By surgical laparotomy on the right flank, all of these seven calves were proven to have abomasal volvulus in either of four different modes. Thus, they underwent only the surgical reduction following removal of the gas and fluid accumulated in the abomasum. Six of the seven calves have responded well to these surgical interventions, but one died of peritonitis fourteen days after surgery. The intensive fluid therapy with Ringer's and 5% dextrose solutions before, during and even after surgery was concluded to be significantly effective in the successful treatment of these cases.
Transfusion with neutrophils of cattle recovered from Theileria sergenti (TS) infection was performed to examine the effects on the percentage of parasitized erythrocytes (PPE) in peripheral blood of TS infected cattle. A cow recovered from an artificial infection with the Fukushima strain of TS showing the persistent low PPE was selected as a donor. Leukocytes were obtained from 1 liter of a donor cow's blood and washed 3 times with a buffer solution by centrifugation. These leukocytes (more than 109 cells) were intravenously injected twice to the recipient cows at 3-day intervals. PPE in recipient cows rapidly decreased, but increased again to the previous value within a few days. The leukocyte transfusion was more effective when injected 5 times. However, the neutrophils from cattle not infected with TS showed no effects on PPE in the recipient cattle. Culture supernatant of co-culture with neutrophils from the donor cow recovered from TS infection and parasitized erythrocytes from the recipient cow was injected 8 times demonstrating no effects on PPE in the recipient cow. The PPE in the recipient cow was rapidly decreased after the blood transfusion from the donor cow as was the neutrophil transfusion. These results suggested that the neutrophils of cows recovered from TS infection were effective to reduce the PPE in the cows highly infected with TS.
A total of 19 cases of hyena disease occurred on a calf beeding farm at Shiga Prefecture from June, 1984 to May, 1986. Clinically, they showed a marked growth retardation in the hindguarters and a disturbance of locomotion which became apparent at five months of age. Autopsy of the 5 casres revealed shortening of the long bones. The epiphyseal cartilage of the shortened long bones was partially deformed and fading. Histologically, chondrocytes in the growth plates decreased in number and showed an abnormal arrangement. Serological tests carried out on 14 of the 19 cases of hyena disease and apparently normal 5 cattle on the same farm revealed no definite relationship between the occurrence of the disease and infections with bovine vial diarrhea-mucosal disease virus, infectious bovine rhinotracheitis virus, parainfluenza 3 virus and bovine adenovirus serotype 7. Biochemically, the serum level of iron increased, and in the affected bones, the Ca level decreased, while the P level increased.
The agglutinin having affinity for cattle red cells was found in Feijao chumbinho lectin. The red cells agglutinated by this lectin were Fc type and those not agglutinated were fc type. The phenotype frequencies of the Fc type were estimated to be 41.75% for Japanese Black cattle and 35.71% for Japanese Brown cattle. The genetic study on the Fc system proved that the inheritance of this system was controlled by a dominant allele at an autosomal locus. The gene frequencies of Fc were 0.237 for Black cattle and 0.197 for Brown cattle. These results provide useful information for the research of individual differences and population genetics.
One case of nodular nonsuppurative panniculitis in a dog is described. The inflammation was observed in a 9-year-old, male maltese. The recurrent and multiple signs and symptoms appeared subcutaneously as a swelling of the liver and spleen. Claudication and fever broke out at the same time. Histophathological findings of skin lesions consisted of granuloma tous inflammation. The adipose tissue almost entirely disappeared. It was substituted by dense inflammatory cells. The inflammatory cells were composed predominantly of hystiocytes. Furthermore the accumulation of phlogocytes and neutrophils were observed in some parts. The fibrous parts were rarely observed. Therapy consisting of a single administration of predonisolon was effective on all symptoms including the skin induration. The recurrence has not been observed. Nevertheless, continuous observation is considered necessary as a few studies on recurrence have been reported.
In March of 1986, a cow on a dairy farm in Fukuoka Prefecture became ill displaying mainly nervous symptoms. She was treated with antibiotics but later slaughtered because of a poor prognosis. The results obtained by the hematological and biochemical examinations suggested a bacterial infection. An autopsy revealed the formation of a pigeon-egg sized abscess in the bottom of the brain. Histologically, necrosis, intense infiltration of neutrophils and bacterial clumps were observed around the hypophysis. Actinomyces pyogenes (A. pyogenes) was isolated from the lesion. A serological survey on A. pyogenes infection was performed with other cows on the same farm. As a result, A. pyogenes protease antibody was detected in 17 out of 19 cows.
Fallot's tetralogy with right ductus arteriosus was detected in a Holstein calf. The right ductus arteriosus measured approximately 6mm in outside diameter. It arose from the pulmonary trunk immediately before the bifurcation into the right and left pulmonary arteries. It ran anteriorly meandering on the right side of the aortic arch and drained into the brachiocephalic trunk immediately before the ramification of the left subclavian artery. The aortic arch was normally formed by the left fourth aortic arch. This ductal anomaly was considered to have developed at the early formative stage of the circulatory system as a rudiment of the distal portion of the right sixth aortic arch.