Eighteen beagles spontaneously infected with Microsporum canis were divided into three groups: topical group, systemic group and re-infection group. They were hematologically and biochemically examined for comparison with healthy beagles. Few hematological differences were found in these groups. Biochemical profiles on sera in three infection groups were within nomal range. Regarding serum protein electrophoretic analysis, in the infection groups, increases in β2-and γ-globulin and a decrease in the albumin/globulin ratio were noted. These changes seemed to depend upon the extent of cutaneous lesions.
Clinicopathological findings of white muscle disease in three heavy breed foals, in the Tokachi district of Hokkaido were examined. The foals showed the clinical signs of recumbency, difficulty in sucking, tachycardia and tachypnea within one day of birth and then died within 2 days of their onset. Pathological examination revealed severe mydegeneration of the skeletal muscle, tongue and diaphragm, but the myocardium was not affected. Serum enzyme activities (GOT, CPK, LDH) increased markedly. The levels of serum selenium of affected foals and their mares were markedly low, below 65ppb, while tocopherol levels were normal. However, in organs of affected foals there existed very low concentrations of selenium and tocopherol. Selenium content in feedstuffs was deficient at levels below 50ppb in dry matter, and tocopherol levels in most of the samples were also below 3mg/100g in dry matter. It was considered that white muscle disease in the heavy breed foals was caused by selenium and tocopherol deficiency of their mares.
Four cases of abortion occurred consecutively on a dairy farm in Hiroshima Prefecture from January to May, 1988. An aborted fetus and dam's fresh lochia of the last case were examined bacteriologically and serologically as a diagnostic service. Actinomyces pyogenes was isolated purely from the lungs, brain, spinal cord, thoracic fluid, contents of digestive organs, and lochia, and it was presumed to be the causative agent in this abortion. However, the aborted fetus could not be examined histopathologically due to severe autolysis. A serological survey on A. pyogenes infection was carried out on four aborted dams and raising cows at this farm, using the agargel diffusion test for protease antibodies against A. pyogenes. The antibody was demonstrated in high titer as 1: 64 in the serum of he dam that was aborted in May, and it was increased to 1: 128 after one month. Twenty-two out of 30 cows on the same farm were revealed to be positive reacters by this test, and nine cows including the three other aborted dam had levels of A. pyogenes protease antibody titers of >8 in ser
Fecal examination was performed on 1, 012 pigs bred in Ibaraki Prefecture to detect gastrointestinal nematodes. As a result, nematode eggs were found in 132 pigs (13.0%) and the average EPG (eggs per gram of feces) was 85.8. The infection rate in the breeding pigs was apparently higher than in the fattened pigs, the positive rates being 56.2%(59 of 105) and 8.0%(73 of 907), respectively. Three species of nematodes, Oesophagostomum dentatum, Ascaris suum and Trichuris suis, were identified in this examination. Of them, O. dentatum was the most predominant, the positive rate of which was 50.5% in breeding pigs and 2.2% in fattened pigs. The other two species were recorded in less than 6% of the pigs. Accordingly, treatment with anthelmintic to breeding pigs seems to be necessary to reduce the occurrence of gastrointestinal nematodes.
Spondylolisthesis was diagnosed in commercial broiler chickens of a certain line on three farms of Okayama Prefecture in September and October, 1987. Clinical signs of the affected birds, lameness and sitting on their hocks and keel, occurred from around 20 days of age, and were prominent at approximately 40 days of age. Such signs persisted until their processing age, showing a total morbidity of 3.6% to 4.4%. The radiography of the birds revealed various degrees of deformation of the 5th to 7th thoracic vertebrae, particalarly dorso-ventral rotation of the 6th thoracic vertebral body. Grossly, a dislocation of the 6th thoracic vertebral body, resulting in spinal cord compression, was noticed. Although the etiology of the present incidence was unknown, it was suggested that a genetic factor played a role in the occurrence of this disease.
In order to develop an inactivated cell culture vaccine, the RC·HL strain was produced by adapting the RCEH strain to HmLu cell culture after a long term cultivation in Vero cell culture, and by limiting dilution which was performed at passages 21 to 23. The RC·HL strain increased the infectivity and growth capability in CRFK, ESK, Vero and HmLu cell cultures, and also increased the pathogenicity for suckling mice and hamsters; however, the strain was non-pathogenic for adult mice, guinea pigs, rabbits and dogs. The RC·HL strain attained a higher infective titer at 32°C-34°C than at 37°C and at an input multiplicity of infection of 1.0 or 0.1 than at 0.01 or 0.001. The inactivated RC·HL strain reached peak immunogenicity one to two days after the virus attained its maximum infective titer. The virus was purified by two methods, and the virus recovery and protein reduction rate were compared between the two. A method using 10% polyethylene glycol (PEG) was more favorable for the purification of the virus than the ultrafiltration method. The purified virus was inactivated with 0.01 M binary ethylenimine (BEI) or 0.0125%β-propiolactone (BPL) within 30 hours, whereas 0.075% formalin required 7 days for inactivation. The immunogenicity of BPL-inactivated virus was most stably maintained during 8-month storage at 4°C. Based on the data collected, it was considered that the RC·HL strain may be usedas a seed virus for an inactivated cell culture vaccine against rabies.
Morphological investigation of the intraerythrocytic stage of Babesia gibsoni was undertaken in parasitized erythrocytes obtained from 26 naturally infected dogs. Many parasitized erythrocytes showed a single parasitization, and in about half of them ring form organisms were noted. Other forms, however, were observed in all cases examined. Multiple parasitization of a single erythrocyte with two or more parasites was also revealed in 22 cases. As a result of the present study, it appeared that morphological variance is commonly present in the erythrocytic stage of Babesia gibsoni.