The objective of this experiment was to clarify the effective treatment for beef cattle with unobserved estrus. A total of 99 Japanese Black cattle which showed unobserved estrus and had a palpable corpus luteum were divided into three groups; GroupI, consisting of 19 cattle injected with 10ml of vitamin AD3E (vitamin A 5, 000, 000 IU, D3 500, 000 IU, E 500mg) and Group II, 31 cattle with 12-15mg of PGFα, All cattle of both Groups I and II were recommended to inseminate at observed estrus after the treatment. Group III consisted of 49 cattle injected with 12-15mg of PGF, followed 54 hours later with an injection of 100μg LH-RH-A. They were recommended to inseminate at 24 hrs after the administration of LH-RH-A. The numbers of cattle which were inseminated within 10 days of treatment were 2 (10.5%), 20 (64.5%) and 49 (100%) in Groups I, II and III, respectively. The percentage of treated cattle pregnant for this 10 day-period was highest in the cattle of Groupe III (57.1%) when compared with Groups I (5.3%) and II (38.7%). It was considered that timed insemination following the injection of PGF2α in combination with LH-RH-A is applicable to cattle with unobserved estrus.
The concentrations of serum α1-acid glycoprotein (α1-AGP), sialic acid and mucoprotein were estimated in 145 cattle with neoplastic and inflammatry diseases, and 10 normal cattle. The mean values of serum α1-AGP were 330.5±104.5μg/ml in normal cattle, 897.8±500.8μg/ml in calf type leukemia, 848.3±826.5μg/ml in thymic type leukemia, 1353.2±679.6μg/ml in adult type leukemia, 480.0±353.6gg/ml in skin type leukemia, 4050.0μg/ml in myelocytic leukemia, 702.8±339.4μg/ml in solid tumors, 1065.6±707.4μg/ml in BVD· MD, 1411.8±862.5μg/ml in traumatic pericarditis, 1987.3±1387.1μg/ml in chronic endocarditis, 1471.7±939.5μg/ml in necrotic mastitis and 1107.5±56.4gg/ml in granulocytopathy syndrome. Serum α1-AGP concentration showed a positive correlation with serum sialic acid and mucoprotein concentration. Serum α1-AGP of necrotic mastitis showed a negative correlation with lymphocyte count, and a positive correlation with serum γ-globulin. As a nonspecific marker, measurement of serum α1-AGP was considered to be useful in the diagnosis of bovine leukosis, solid tumors and inflammatory diseases.
A total of 201 Escherichia coli strains isolated from diseased animals (calf, 119 strains; pig, 82 strains) from 1984 to 1987 were examined for drug resistance and prevalence of R plasmids. In calf strains, percent resistances to each drug were as follows: tetracyclin (TC), 59.7%; chloramphenicol (CP), 21.0%; streptomycin (SM), 52.9%; sulfadimethoxine (SA), 37.8%; kanamycin (KM), 45.4%; aminobenzyl penicillin (ABPC), 43.7%; and nalidixic acid (NA), 28.6%. In pig strains, percent resistances to each drug were as follows: TC, 98.8%; CP, 20.7%; SM, 63.4%; SA, 80.5%; KM, 67.1%; ABPC, 41.5%; NA, 2.4%; and trimethoprime (TMP), 6.1%. Of the 164 drug resistant E. coli strains (82 calf strains, 82 pig strains) examined, 88 (53.7%) were found to carry R plasmids, capable of performing a conjugal transfer.
Recently, strains of Haemophilus paragallinarum (Hpg) and Mycoplasma gallisepticum (MG) have been isolated from commercial broilers showing respiratory signs. This poultry farm was raising about 30, 000 chickens of the same age in a crowded egg and broiler production area in Miyazaki Prefecture of Japan. Respiratory signs appeared in the flock at 50 days of age, morbidity was 15% and mortality was 11% till 63 days of age prior to marketing. Three 63-day-old chickens and 20 serum samples each of 35-, 49-and 63-day-old chickens were examined. All the three chickens examined showed facial edema and nasal discharge, while Hpg (A type) and MG were isolated from respiratory organs. Hemagglutination-inhibition antibodies against Hpg (A type) were detected in 63-day-old samples, and agglutination antibodies against MG and Mycoplasma synoviae were detected in 49-and 63-day-old samples. When 3 broilers (49-day-old) were inoculated intranasally with Hpg isolates, all of them showed facial edema and nasal discharge 5 days after the inoculation. The present paper may be the first report describing the respiratory disease complicated with infectious coryza and MG infection in commercial broilers in Japan.
The closure of the ductus venosus (DV) was studied in 59 cattle, ranging from a 249-dayold fetus to a 183-day-old calf. In the 249-day-old fetus, the DV was a large vein located within the papillary process of the liver. The DV originated at the confluence of the umbilical and portal veins and emptied into the posterior vena cava. At the origin of the DV, there was a thin circular fold consisting of immature connective tissue. Obliteration of the DV always started at this origin and proceeded toward the posterior vena cava. There was a considerable variation in the time of the closure of the DV. In most cases, however, the origin of the DV was obliterated within two weeks of birth.
A case of glioblastoma was found in a 6-month-old Holstein cow. The animal showed ataxia and had a neoplastic nodule occupying a part of the lower cerebellum and the cerebellar peduncle. The neoplastic tissue was composed of pleomorphic cells frequently displaying mitosis and was accompanied by necrosis and haemorrhage. There were many blood vessels and a considerable amount of collagen fibres in part of the neoplastic tissue. Some neoplastic cells were stained positively with anti-glial fibrillary acidic protein (GFAP) serum, and a large number of cells revealed S-100α or S-100β positivity. Neuron-specific enolase (NSE)-positive cells were rarely seen. The histological and immunohistochemical features in the present case are discussed in comparison to those in other primary neoplasms of the nervous system.
A 3-month-old female West Highland white terrier (case 1) who had a cardiac murmur and mild dyspnea and a 3-month-old male Yorkshire terrier (case 2) who had a cardiac murmur were presented at Azabu University Teaching Hospital and examined because of cardiac diseases. Utilizing physical examination, X-ray, electrocardiogram (ECG) and echocardiogram in the first visit, case 1 was diagnosed as pulmonary arteriostenosis (PS) and case 2 was diagnosed as aortic stenosis. We followed up case 1 for 21 months and case 2 for 15 months. Case 1 gradually became cyanotic after exercise at 16 months of age and case 2 showed no clinical abnormality. In the X-ray examination, neither case showed any changes. In the ECG, right ventricular enlargement in case 1 and left ventricular enlargement in case 2 were observed, each case being stable. In the echo examination, each case had normal left ventricular function. Case 1 is still alive, however, case 2 died suddenly.