Journal of the Japan Veterinary Medical Association
Online ISSN : 2186-0211
Print ISSN : 0446-6454
ISSN-L : 0446-6454
Volume 46 , Issue 2
Showing 1-14 articles out of 14 articles from the selected issue
  • Shinji TAKAI
    1993 Volume 46 Issue 2 Pages 91-97
    Published: February 20, 1993
    Released: June 17, 2011
    JOURNALS FREE ACCESS
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  • Hiroshi AMANO, Norikazu KAJIO, Mamoru TSUCHIYA, Masatoshi SHIBATA, Sac ...
    1993 Volume 46 Issue 2 Pages 99-102
    Published: February 20, 1993
    Released: June 17, 2011
    JOURNALS FREE ACCESS
    In November, 1988, 41 of 107 SPF pigs showed pyrexia, anorexia and respiratory signs on an SPF farm which started breeding of SPF pigs 3 months before the occurrence. Ten of the pigs died one to three days after the onset of clinical diseese. At autopsy, a large quantity of yellowish cloudy fluid containing fibrinous exudate was found in the abdominal, thoracic and pericardial cavities. Histopathologically, purulent meningitis, purulent fibrinous polyserositis (pleuritis, pericarditis and peritonitis) and purulent arthritis were observed. Haemophilus parasuis was isolated from some organs, cavity fluid and synovial fluid in five of 11 pigs examined. The isolates were identified as serotype 5 by the gel diffusion method and as PAGE type II by SDS-polyacrylamide gel electrophoresis. These results indicate that the present outbreak was of Glasser's disease. The results of serological examination suggest that the organism may have invaded the SPF farm, and cold stress may have led to a serious enzootic outbreak of the disease with a high morbidity.
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  • Yukio OKADA, Fumio HARA, Nobuo NII, Masami HARA, Takasi ANZIKI, Tadaak ...
    1993 Volume 46 Issue 2 Pages 103-107
    Published: February 20, 1993
    Released: June 17, 2011
    JOURNALS FREE ACCESS
    Properties of raw milk, which derived from dairy cows having no antibiotic treatment but showed inhibitory activity against Bacillus stearothermophilus in a paper disk test (PDT), were studied cytologically and biochemically. The total number of cells, the number of polymorphonuclear leukocytes (PMN), and the concentration of lactoferrin (Lf) and lysozyme (Ly) in PDT-positive milk were higher than in PDT-negative milk. A correlation was found between the diameter of the inhibition zone and the total number of cells, the number of PMN, or the concentration of Lf and Ly. The Lf showed the highest correlation with the diameter of the inhibitory zone. The egg Ly showed the highest inhibiting activity against the bacterial suspension when the suspension was examined by the optical density, followed by PDT-positive whey, human Lf, PDT-negative whey, and penicillin. When bacterial cells were observed by transmission electrom microscopy, morphological changes caused by PDT-positive whey resembled those of human Lf. The results indicate that the PDT-positive reaction with milk with no antibiotic residue is caused mainly by Lf derived from PMN originating from mammary gland inflammation, and that the reaction is accelerated by the presence of Ly.
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  • Shyoji KUMATA, Sota HOMMA
    1993 Volume 46 Issue 2 Pages 108-111
    Published: February 20, 1993
    Released: June 17, 2011
    JOURNALS FREE ACCESS
    Bovine blood selenium (Se) was determined by high performance liquid chromatography (HPLC) with fluorescence detection. After the digestion of blood samples with nitric acid-perchloric acid (1: 1) at 185±5°C for 3 hr, Se (VI) in the solution was reduced to Se (IV) by hydrochloric acid in a boiling water bath. The compound (Se-DAN) derived from the reaction of 2, 3-diaminonaphthalene (DAN) and Se in the solution was extracted with cyclohexane. Cyclohexane was injected into HPLC preparing silica gel for the stationary phase and a solvent of cyclohexane and ethyl acetate (93: 7) for the mobile phase, whereupon Se-DAN was detected with the excitation at 380 nm and the emission at 525 nm. The coefficient of variation of the determined values in whole blood and serum Se (n=10) was below 2%. The calibration curve was linear and its correlation coefficient was 0.999. The recovery rates of Se added to the whole blood or serum were 100±5%. This method is considered convenient, excellent in repetition and suitable for serial determination of Se in a large number of bovine blood samples.
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  • Tetsuo SEKINO, Togo SAMEJIMA, Masanori TAJIMA
    1993 Volume 46 Issue 2 Pages 112-116
    Published: February 20, 1993
    Released: June 17, 2011
    JOURNALS FREE ACCESS
    An enzyme-linked immunosorbent assay (ELISA) using purified equine infectious anemia (EIA) virus antigen was developed for the detection of antibodies to EIA virus. The purified antigen was analyzed by SDSpolyacrylamid gel electrophoresis, and 26 kilodalton polypeptide (26 K) was predominantly detected. In order to define the minimal absorbance reading (MAR) for a positive reaction, sera collected from 5 horses experimentally infected with EIA virus were examined by western blotting (WB) for the appearance of specific EIA antibody. Positive reaction against the 26 K was first observed at 18 days post-infection in 2 horses. Since these two sera showed an absorbance reading of approximately 0.5 by the ELISA, the MAR was established as 0.5. A serum which had been adjusted to give an absorbance reading of approximately 0.5 was applied as a reference, and test-samples showing an absorbance reading greater than that of the reference serum were judged to be positive. The detection of antibodies by the ELISA was compared with that by the agar gel immunodiffusion (AGID) test. The rate of coincidence between the tests was 90% in sera from horses with experimentally induced EIA and 99.6% in sera from horses in the field. Sera not in agreement were confirmed by WB, and the ELISA was evaluated to be more sensitive than the AGID test. Only 0.4% of the sera tested gave false-positive results by the ELISA. The present results indicate that the ELISA is a useful test for detecting antibodies to EIA virus.
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  • Kazuki ISHIBASHI, Hitomi SHIRAKAWA, Yoshifumi TOMISHITA, Hiroyuki MATU ...
    1993 Volume 46 Issue 2 Pages 117-119
    Published: February 20, 1993
    Released: June 17, 2011
    JOURNALS FREE ACCESS
    Indirect fluorescent antibody (IFA) technique using a monoclonal antibody (M-Ab) specific to nucleocapsid protein of avian infectious bronchitis (IB) virus was applied to diagnosis of IB of chickens. Since affected chickens of 69 days of age showed respiratory signs and severe diarrhea, they were suspected of IB clinically. The IB virus specific antigens were clearly demonstrated in thin sections of the swollen kidneys collected from chickens. The IB virus was isolated from the kidneys and virus neutralizing antibody titers against the isolates rose significantly in convalescent sera of chickens. From these results, the disease was diagnosed as IB, and the IFA technique using M-Ab was judged to be usuful for diagnosis of IB.
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  • 1993 Volume 46 Issue 2 Pages 120-136
    Published: February 20, 1993
    Released: June 17, 2011
    JOURNALS FREE ACCESS
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  • Matsunari KAJIYAMA, Midori NAKAMOTO, Takashi MAKITA
    1993 Volume 46 Issue 2 Pages 141-142
    Published: February 20, 1993
    Released: June 17, 2011
    JOURNALS FREE ACCESS
    Pharyngostomum cordatum is one of the parasites in the small intestine of animals of the Felidae family, as a final host. It is an important subject in public health to determine whether P. cordatum is a cause of zoonosis. For this, metacercariae of P. cordatum were collected from the hind leg muscles of naturally infected Bufo and orally administered to four Japanese monkeys, three males and one female.These monkeys, given 450, 215, 82 and 105 metacercariae, were autopsied after 38, 32, 32 and 40 days, respectively. The feces of each monkey were examined every day. None of these monkeys had P. cordatum in the intestine, diaphragm or abdominal organs. These results strongly suggest that the monkey may not susceptible to P. cordatum infestation.
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  • Shigeru TAKANO, Yumi KONDOH, Toshifumi ASAI, Shigeru MIKAWA
    1993 Volume 46 Issue 2 Pages 143-146
    Published: February 20, 1993
    Released: June 17, 2011
    JOURNALS FREE ACCESS
    An outbreak of a disease characterized mainly by depression and torticollis was observed in six rabbits reared on a resort farm. From the findings of the pathological and parasitological examinations, three of them were diagnosed as having encephalitozoonosis. Regarding clinical signs, depression and torticollis were noticed in one rabbit each while nasal discharge and sneeze were observed in all the rabbits. On the 7th day of observation, the rabbit with torticollis began to show incoodination. The autopsy disclosed no marked change but white spots in the kidney of one rabbit and sinusitis in two. Histological examinations revealed granulomatous encephalitis and interstitial nephritis in three rabbits. In one of them, pseudocysts were confirmed in the cerebral cortex. Emulsion of the brain and kidney which had histological lesions was intraperitoneally inoculated into mice which had been subjected to immunosuppressive treatment. As a result, protosoan proliferation was observed in ascites. The protozoa was 2.5×1.5μm in size and grampositive. Electron microscopy revealed a 5-6-round polar filament and polarplast lamellae. From these morphological characteristics, the protozoa was identified as being Encephalitozoon cuniculi.
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  • Mitsuyoshi NAKAGAWA, Kazuro MIYAHARA, Motoyoshi SATO, Tsuneo HIROSE
    1993 Volume 46 Issue 2 Pages 147-150
    Published: February 20, 1993
    Released: June 17, 2011
    JOURNALS FREE ACCESS
    A 360° rotary restraint unit for use in small animals was developed to protect against X-ray irradiation in diagnosis in veterinary medicine. X-ray exposure measured at 50 mm from the surface of the omnidirectional protective shielding X-ray system (MMS-105, Hitachi Medical Corporation) with the use of the restraint unit was compared with X-ray exposure using manual restraint. When the abdomen of a middle-size dog administered with positive contrast medium was irradiated 10 times, total X-ray exposures using manual restraint and the restraint unit were 0.32-1.73μSv and 0.00μSv, respectively. Using fluoroscopy, X-ray exposures using manual restraint and the restraint unit were 13.7-41.5μSv/hr and 0.1-0.5μSv/hr (the same level as background radiation), respectively. Therefore, the use of the restraint unit decreased X-ray exposure markedly. No adverse effect to the roentgenogram was observed with the restraint unit. With the restraint unit and with the rotation, accurate positioning with detailed imaging of the animal in less time was made possible.
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  • Kou SAWASHIMA, Keisuke AOKI, Hidetoshi SHITAKA, Yuuko SAWASHIMA, Hiros ...
    1993 Volume 46 Issue 2 Pages 151-154
    Published: February 20, 1993
    Released: June 17, 2011
    JOURNALS FREE ACCESS
    A 15-year-old, female mongrel dog was presented due to loss of general physical conditions with anorexia. From the physical examination, thoracic radiographs and ultrasonography, the dog was diagnosed as primary lung tumor and treated with a combination protocol of vincristine, cyclophosphamide and prednisolone. After 6 weeks of treatment, thoracic radiography showed a remission of the size of the pulmonary mass, while the general conditions did not recover. Lobectomy was then perfomed, and a surgical biopsied sample of the pulmonary mass was examined and diagnosed histopathologically as bronchiolar-alveolar carcinoma. There was no gross and histopathological evidence of local metastasis of the pulmonary carcinoma, but gastric scirrhus carcinoma had metastasized to many organs and tissues systematically. Massive necrosis of tumor tissue, which seemed to be attributable to the chemotherapeutic effect, occurred in the pulmonary carcinoma, but not in the gastric carcinoma.
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  • 1993 Volume 46 Issue 2 Pages 155-158
    Published: February 20, 1993
    Released: June 17, 2011
    JOURNALS FREE ACCESS
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  • Mutsuko SAKUI, Susumu OHFUJI, Hiroyuki TANIYAMA, Hidefumi FURUOKA
    1993 Volume 46 Issue 2 Pages 163-165
    Published: February 20, 1993
    Released: June 17, 2011
    JOURNALS FREE ACCESS
    Systemic amyloidosis was observed in a 2-year-old, Landrace-breed sow. Amyloid deposits were observed in the liver, spleen, kidney, small intestine, and many lymph nodes. These were stained faintly red with hematoxylin-eosin, and yellowish-red with congo red, which showed emerald-green birefringence under polarized light. Amyloid protein was sensitive to the potassium permanganate treatment losing affinity for congo red, and reacted positive for the anti-AA antibody. The present case was diagnosed to be secondary systemic amyloidosis which was immunohistochemically confirmed to be a deposit of AA protein, associated with granulomatous mastitis caused by Actinomyces sp.
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  • 1993 Volume 46 Issue 2 Pages 166-168
    Published: February 20, 1993
    Released: June 17, 2011
    JOURNALS FREE ACCESS
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