In healthy Holstein dairy cows the milk fat and protein ratios were minimum at the peak of lactation. While the highest fat content was observed immediately after partition without significant changes in protein content for about 3 months post partition. The proportion of milk protin ratio to milk fat ratio (P-F ratio) was 86.7% on an average. In cases with fat cow syndrome, the milk volume, fat content, protein ratio and protein content were lower than those of healthy cows, whereas the milk fat ratio was higher. The postpartum P-F ratio was significantly decreased in the affected cases. It was indicated that changes in the balance of milk components might be exactly evaluated from P-F ratio together with milk fat and protein ratios and contents.
A dairy Holstein cow showed copious vaginal dischage, swelling of the udder, edema of the vulva and decrease of milk production at 221 days of gestation, and abortion occurred 7 days later. At necropsy, extensive hemorrhage and localized edema were seen in the fetal placenta, and muddy yellowish green mucous fluid was present in the fetal abomasum. Histopathologically, there were hemorrhagic and necrotic lesions in the placenta with Grampositive cocci within epitheals as well as caterrhal inflammation in the fetal small intestine and abomasum.Streptococcus suistype 15 was isolated from the placenta, fetal small intestine and abomasum, and vaginal swab of the dam.
After intratracheal inoculation into 6-week-old guinea-pigs with various doses ofActinobacilluspleuropneumoniaeserotype 1, strain HA-337, most animals showed depression, anorexia and dyspnea within one week, and 2 of 3 animals infected with 107CFU died on day 6 postinoculation. The gross lesions were located mainly in the apical and cardiac lobes of the lungs, and histology revealed purulent bronchopnemonia but no fibrinous pleurisy in all infected cases. The organisms were isolated from all animals infected with 107CFU, and one each infected with 105and 103.
With a total of 108 canine sera, the antibody titers by immunoperoxidase (IP) method and those by neutralizing test (NT) to canine distemper virus (CDV) showed a correlation coefficient of 0.910. In 4 dogs, which received CDV vaccine, the kinetics of IP titers were almost similar to those of NT titers. The antisera to anine parainfluenza virus, adenovirus, coronavirus and parvovirus did not react with CDV antigen by IP method.
The efficacy of 1% pimaricin suspension was evaluated to canine otitis externa related with yeast-like fungi. Dogs were diagnosed as otitis externa associated with the fungi from clinical examination using a otoscope and microbiological tests. This drug was administrated 4 or 5 drops into ear canals twice a day for 2 weeks. One hundred and forty-five cases were evaluated for the safety and 70 of them were evaluated for the clinical efficacy. Clinical responses were classified into 4 categories; very good, good, fair, and poor. In 145 cases examined, no significant side effects were observed during the therapy. The symptoms of otitis externa were ameliorated steadily by the therapy. After the therapy, 16 cases belonged to the group of very good response, 35 cases belonged to good, 12 cases belonged to fair and 7 cases belonged to poor. Satisfactory responses were clinically observed in 72.9%.
The prevalence of antibody toEhrlichia caniswas investigated in dogs in Kyushu Island, Japan by indirect fluorescent antibody (IFA) test using an Oklahoma isolate grown on DH82 cells as the antigen. Of 622 dogs without any noticeable clinical signs, 12 (1.9%) showed the IFA titers of 1: 20 to 1: 320. Of four patient dogs with fever, anorexia, weight loss, extreme weakness, epistaxis or pancytopenia, one showed the IFA titer of 1: 80.
From March 1991 to May 1993 a total of 500 cats in Kagoshima area was surveyed for helminths and antibody to Toxoplasma gondii. The positivity rate for helminths was 66.8%, being 79.3% of youngs and 64.4% of aged, and 68.5% in males and 66.0% in females. Eight species of helminth were detected: Pharyngostomum cordatum, Dipylidiumcaninum, Spirometra erinacei, Toxocara cati, Ancylostoma tubaeforme, Dirofilaris immitis, Ancylostoma kusimaenseandUncinaria sp.A. kusimaenseandUncinariasp. were the first cases reported in Japan. Antibodies to T. gondii were detected in 16.0% of cats surveyed.
A 6-month-old female slaughter pig clinically showed pyrexia, polypnea and dysstasia with a deep stab wound in the muscles around the right scapula. Autopsy revealed that the affectedmuscles of the right shoulder to the back were dark reddish-brown in color, which were spongy with numerousgas bubbles and a rank odor. Histologically, coagulation necrosis was observed in the affected muscle fibers, and grampositive large bacilli were seen in the interstitium with edema and thrombophlebitis. An obligatory anaerobic rod was isolated from the affected muscles and lymph nodes, and the isolate was identified asClostridium novyitype A by physiological and biochemical characteristics.