The base and apex diameter and length of the teats as well as the diameter and length of the canals and the teat gradient (base diameter-apex diameter) of 24 and 25 mammas from culled and lactating cows, respectively were measured, and these parameters were compared between the normal and abnormal groups according to milk mastitis tests and bacteriological examination. The teat gradient and canal diameter were significantly larger in the abnormal group than in the normal. The teat canal diameter of the bacteriapositive group was significantly larger than that of the negative group, suggesting that the larger teat gradient and/or canal diameter might be liable to mastitis.
Nasal swab-samples and sera from 103 piglets and pigs, aged 2 to 12 weeks, which were reared in 6 farrowto-finish herds in Kumamoto and Oita Prefectures, were examined for carrying Bordetella bronchisepticaand Pasteurella multocida. B. bronchiseptica was isolated from 26 nasal samples from pigs having serumagglutinating titers lower than 1: 160. P. multocida was isolated from 55 pigs whose serum agglutinintiters to B. bronchiseptica ranged from less than 1: 20 to 1: 1, 280. In 3 of 6 herds, P. multocida wasisolated from young age-groups without previous isolation of B. bronchiseptica, suggesting that some cases mightcarry P. multocida in the nasal mucosa without preceding colonization of B. bronchiseptica.
An outbreak of fibrinous and necrotizing pleuropneumonia with edema, hyperemia and induration of the lungs, was observed in 128-and 140-day-old piglets in an integrated farm in Aomori Prefecture in October 1992. In the lung lesions of dead cases serotype 8 antigen of Actinobacillus pleuropneumoniae was detected by immunohistochemistry, and the serotype 8 organisms were isolated from the dead cases as well as slaughtered cases from the same farm. All the isolates were sensitive to all antibiotics tested. Positive rates of complement fixing antibody to the serotype 8 organisms were 30% and 87% in breeding sows and slaughtered pigs, respectively, and 4 of 6 neighbour farms tested were also sero positive (17-71%).
Meronts, gamonts and oocysts were detected in the epithelium of the bile duct and gall bladder of dead Japanese quail Coturnix coturnix japonica from two different sources, and mature oocysts were isolated from the bile. Sporulated oocysts were morphologically identified as those of an Eimerian species. After inoculation of quail chicks with a small number of oocysts, the protozoan parasites were seen only enterocytes, while also in epithelial cells of the bile duct and gall bladder after inoculation with a large number of oocysts. The present species from quails seemed to be of E. bateri Norton and Peirce, 1971 in characteristics.
Of 108 (65 from dogs and 43 from cats) bacterial isolates from 30 of 46 dogs (65%) and from 20 of 34 cats (59%) having ocular diseases in Kanagawa Prefecture from June 1990 to January 1991, staphylococcal species constituted the majority, including of S. intermedius, S. hyicus subsp hyicus and S. hyicus subsp chromogenes. There isolates were most highly sensitive to gentamycim, followed by erythromycin, oxytetracycline and chlorampenicol.
To investigate the feasibility of clinical discrimination on the lethal progression of Babesiagibsoniinfected dogs, the changes in serum enzyme levels, the serum enzyme activities, isozymes andhistopathological data were analysed with experimentally infected dogs. In the dogs died with the ingravescence, the activity of the liver isozyme of alkaline phosphatase (ALP) was found to markedly increase due to the retention of bile in the bile duct of liver and hepatocytic necrosis. This indicated that whether infected dogswould proceed into a fatal condition or not can be determined by ascertaining the increased level of serum ALP.
A 2-month-old kitten which lost consciousness after faling from a height with a transient recovery and got worse without responses to any medical treatment, was subjected to craniectomy of the left parietal bone for cerebral decompression and the postoperative state was satisfactory.
An outbreak of tuberculosis occurred in a deer farm in Aomori Prefecture. In 1988a total of 288 animals of Cervus unicolor and Cervus nippon were introduced from Southeast Asia, and 136 (47.2%) of them and 96 of 146 (65.8%) self-bred deers died within 3 years. Tuberculous lesions have been formed in the lung, pulumonal lymph nodes and various organs of 30 autopsied cases, and histopathology revealed exudative and proliferative tuberculous lesions. Mycobacterium bovis was isolated from lung lesions of 6 cases examined.
Three cats were fed mouse brain containing 1.1-1.4×103 cysts of the K strain of toxoplasma, and they were then injected intramuscularly with prednisolone (10 mg/kg/day) for 10 days. They given prednisolone showed systemic apparent infection with parasitemia and excretion of oocysts and died 10 to 14 days after inoculation. Autopsy revealed typical lesions as seen in natural feline toxoplasmosis and tachyzoites were detected in the lung lymph node and the various organs. A cat given no prednisolone survived infection with a transient parasitemia and oocyst excretion for 9 days.