Pregnant cows were administered 13-carotene and vitamin A in feed for 8 weeks before parturition, and the blood and milk of cows and blood of newborn calves were examined for the druglevels. In cows given β-carotene alone its serum and colostrum level markedly increased, and newborn calves showed markedly increased levels of serum, 8-carotene and vitamin A. Those given both carotene and vitamin A, showed high levels of serum vitamin A, showing no increased levels of serum retinylpalmitate. The results suggested that an administration of β-carotene alone in feed was effective in utilization of both carotene and vitamin A in cows, whereas combined administration of β-carotene and vitamin A had poor effect.
After a single subcutaneous injection with 1 mg of a long-active prostaglandin F2α(PGF2α) analogue, Fenprostalene (A-75), into cows with the retained placenta by 8 hours after expulsion of fetuses, the fetal membrane was expelled within 4 days in 9 of 27 cases (33.3%). In the other group of cows with lochiometra, which showed fever and poor appetite for 4 to 14 days after parturition, showing the uterine horns 8 or more fingers in width. Eleven of 16 cases (68.8%) expelled a large quantity of lochia within 3 days after treatment with A-75, and clinical recovery was seen with the uterine involution. In another group of cows with pyometra and the uterine horns 8 or more fingers in width sinking across the abdominal cavity for 37 to 165 days after parturition, all the cases expelled a large quantity of pus within 3 days after A-75 treatment, resulting in the uterine involution.
A 7-day-old piglet with diarrhea showed the slightly thin wall and watery contents of the large intestine at necropsy. Histologically, numerous Gram-negative bacilli adhered to the luminal surface of enterocytes in the gyri centripetales of the colon. Scanning electron microscopy revealed that the microvilli of epithelial cells in the area were lost. The organisms were immunohistologically positive for Escherichia coli O group 119 antigen and considered as attaching and effacing E. coli (AEEC).
The accuracy of a latex agglutination (LA) test kit for detection of circulating adult heartworm antigen was compared with that of a enzyme immunoassay (ELISA) test kit. Thirty-five (83%) and 41 (98%) of 42 heartworm-free dogs were negative by LA and ELISA, respectively, and the specificity in ELISA was significantly (P<0.05) higher than in LA. Dogs with false-positive by LA and ELISA showed no correlation with harboring of intestinal parasites. Twenty-five (68%) and 16 (43%) of 37 heartworm-infected dogs without microfilaremia were positive by LA and ELISA, respectively. The sensitivities in LA were higher than in ELISA in dogs harboring both male and female adult heartworms, male or female adult worms only and immature worms. Out of 35 heartworm-infected dogs with microfilaremia, 34 (97%) and 33 (94%) dogs were positive by LA and ELISA, respectively. The degree of reaction in both tests seemed to reflect the severity of worm burden, but the correlation was not enough to estimate the number of heartworms residing
An about 8-year-old male Shetland sheepdog showed vomiting, salivation, listlessness, anorexia, decending abdominal skin with depilation, pigmentation, swelling breast and nipple, continuous bloody stool and cryptorchism. Thorombocytopenia and prolonged blood coagulation time were obserbed, and two fist-sized masses were detected in the peritoneal cavity by palpation and X-ray. During operation to remove the peritoneal masses, there was considerable local bleeding, and hemorrhagic spots were seen in the colon, and the mesentery. After operation, the dog was anemic continuously excreting bloody stool, and died in 15 days. Pathological diagnosis was Sertoli cell tumor with seminoma.
In relation to massive infection with Salmonella Typhimurium occurring at a nursery school, the persistence of the organisms in the sand and the effect of disinfectants were investigated. The organisms survived for months period in the sand and the bacterial growth was remarkable in the sterilized sand after inoculation. The organisms persisted for about one week on the tile floor. Compound solution of cresol and sodium hypochlorite was effective in sterilizing the organisms in the sand.
Deep pectoral myopathy was detected in 10 cases of 52-to 58-day-old broiler chickens. The lesions were bilaterally recognized only in the deep pectoral muscles showing greenish discoloration and atrophic degeneration. Histopathology disclosed ischemic Zenker's degeneration with proliferation of newly formed multinuclear muscle fibers and some fibrous tissue. Lymphocytic perivenulitis was noted.