A male Japanese black calf born by the embryo transfer showing diarrhea and common cold-like symptoms at 4 days of age, died 7 days later. At necropsy there were scattered dark-red spots in the lungs, multiple dark-red spots in the small intestinal mucosa and swelling of some mesenteric lymphonodes. The thymus was remarkably atrophied. Histology revealed the absence of Hassal bodies, multiple necrotic foci in the liver, cattarrhal bronchopneumonia and multiple necroses in the tunicapropria in the whole intestinal mucosa. In these lesions and the esophagal mucosa, yeast cells and short fungal pseudohyphae were detected by Grocott and PAS stains, and yeast cells were positive for antibody to Candida spp. by immunostain. Candida tropicalis was isolated in pure culture from the lungs, liver and mesenteric lymphonodes.
The body weights (BW) of 14 heifers with pointed metallic foreign bodies (PMFB) in the reticulum were lower than those of 45 heifers without PMFB. After administration of the rare earth magnet, the daily weight gain was improved in the 14 heifers with PMFB, reaching in 120 days almost the BW of 45 heifers same level as those without PMFB. The effect was more remarkable in heifers with PMFB sticking to the reticular wall.
The tube agglutination test for serodiagnosis of equine paratyphoid gave different results depending on diluent of test sera and antigen concentration. Using physiologic saline as serum diluent and a 1: 15 dilution of antigen, out of 1, 066 serum samples, 10 from infected horses showed positive titers of 1: 1, 280 or higher, while others from noninfected horses did titers of 1: 640 or lower. Agglutination pattern was more clearly seen after heating of the reaction mixtures at 50°C for 2 hr and then keeping at 4°C for 18 hr than incubating at 37°C for 18 hr.
The injectable or tableted enrofloxacin (ERFX, 5mg/kg) was administered to 3 adult dogs, and urine or s erum (only with tablet) levels and binding rate to serum protein in vitro were measured. The urinary levels a fter injection and tablet medications were the same, showing peak levels of 72.3 and 78.0 μg/ml at 0-6 h rs, with continued high levels of 8.5 and 12.1 μg/ml at 12-24 hrs after subcutaneous and oral administrati ons, respectively. After tablet administration, serum level reached a peak of 1.8 μg/ml at 1.3 hrs, declining la ter with a half-life of 3.0 hrs and the AUC0-24 of 11.0 μg·hr/ml. The in vitro protein binding rate of ERFX w as 32.6%.
Urinary and serum concentrations of enrofloxacin were studied in 3 adults cats after subcutaneous and oral administration of 5mg/kg of the injectable or tablet formulation. Mean urinary concentrations of 125-140μg/ml were maintained from 6 to 24hrs after oral administration. After subcutaneous administration, the increase in concentration was rather slower than oral route, but showing a higher peak level of 217μg/ml at 12 hrs. The 48hr-cumulative recovery rates were similar in both routes of administration. The serum concentration rapidly increased after oral administration reaching a high peak level of 2.9, μg/ml. The undercurve area was 21.4μg · hr/ml and the half life was 5.9hrs. The in vitro serum protein binding rate was 35.9%.
A 2-year-old female mongrel dog showing chronic osteomyelitis following open fructures on the left distal radius, ulna and carpal joints was treated with osteotomy of the exposed dead bones, fixation with a K-E splint and cancellous bone autograft. The consequent atrophic process was improved and functonal bony union was achieved after additional superficial osteoparacentesis and cancellous bone regrafting at the fracture site.
Slaughtered pigs were subjected to isolation of Erysipelothrix rhusiopathiae and determination of antibody titers of joint fluid by growth-agglutination (GA) test. Of 501 pigs with arthritis, 133, 64, 30, 10, 12, 6, 31, 65, 58 and 92 cases showed the GA titers of 1: 2, 1: 4, 1: 8, 1: 16, 1: 32, 1: 64, 1: 128, 1: 256, 1: 512 and 1: 1, 024, respectively. E. rhusiopathiae was isolated from 173 of 246 cases showing GA titers of 1: 128 or higher, but not from 255 cases showing GA titers of 1: 64 or lower. The GA titers of the sera from 60 normal slaughtered pigs as a control were 1: 32 or lower except for 2 cases with 1: 64. Swine arthritis cases with GA titers of 1: 128 or higher were considered to be due to E. rhusiopathiae, while the casses with 1: 64 or lower titers were not.
Fecal samples from 4, 914 slaughtered cattle were examined for verocytotoxin-producing Escherichia coli (VTEC). Eleven strains of VTEC were isolated, and their serotypes were O26: H-(one strain), O26: H11 (four strains) and O157: H7 (six strains). The VTEC-positive cases were distributed in eight prefectures of Japan.