Thirty nine Holstein Friesian bulls raised for meat were examined at 1 to 22 months of age for plasma vitamin A, vitamin D metabolites and vitamin E and liver vitamin A. Plasma vitamin A and 25-hydroxyvitamin D increased with age. Liver vitamin A was lower in younger animals, gradually increasing with age. The levels of plasma 1, 25-dihydroxyvitamin D and alkaline phosphatase were higher at 10.5 months or less of age, suggesting the plasma concentration increasing with the bone growth. Plasma vitamin E was much lower in younger animals, increasing slightly with age.
Three piglets aged 65 days were moved to another pen with other three piglets and the lymphocyte incorporation of 3H-thymidine was measured using pokeweed mitogen (PWM), phytohemagglutinin (PHA) or concanavalin A (Con A). The incorporation was significantly decreased on 1 to 7 days after moving, while such a decrease was not observed in four piglets aged 87.2±1.6 days moved to another pen.
An outbreak of pleuropneumonia occurred on a large scale integrated swine farm rearing 600 sows in August 1991 to 1992, being prevalent in piglets within a week after moving to the raising barn. The mortality was 5% in August 1991, later increasing to 11.3% in April 1992. Pathological examination revealed extensive pleuropneumonia in all dead cases, and Actinobacillus pleuropneumoniae serotype 1 was isolated from the lungs and the pulmonary lymph nodes. The improvement of feeding, enviromental conditions of the raising barn and, in particular, the improved feed mixing of antibiotics lowered mortality to 5% in October 1992.
In January and May 1992, Moraxella catarrhalis was isolated from the lungs of two fatal cases of calves of Japanese Black breed showing purulent bronchopneumonia. Immunohistology revealed M. catarrhalis antigen was demonstrated in the pneumorlic lesions.
From December 1992 to August 1993, 12 stillborn calves and 24 other calves were shown to have enlarged thyroids in two feedlot farms of the first-calving heifers. The thyroids of 3 still-born calves weighed. 250g, 360g and 420g, respectively. Histopathology revealed that the follicular epithelium was of tall columnar cells showing papillary growth and that the follicular lumen was filled with degenerated, and exforiated epithelium without colloid. The thylonine-T3 level was higher in cows and lower in calves of the affected farms while serum lipids and thyloxine-T4 were within the normal range. Average iodine content was significantly lower in the thiroid of still-born calves (5.9 ppm/DM) as well as several feeds examined (0.3ppm/DM) of its farms. The present cases of calf goiter might be due to iodine shortage.
A 4-year-old male cat receiving cardiopulmonary ressuscitation after cardiac arrest because of hypoxia during operation showed severe neurologic symptoms such as confusion, mydriasis, astasia, opisthotonus, head tremor, and loss of menace reaction and less electroencephalogram (EEG) potentials. While the neurologic symptoms gradually recovered in three years, the blindness and less EEG potentials remained.
A rabbit case of bilateral pseudosymblepharon with chameleon eye and partial adhesion was subjected to three operative procedures, which were compared in degrees of the resulting conjunctival hyperplasia and adhesion. The inversion suture of hyperplastic conjunctiva proved to be superior to the other procedures in suppressing adhesion with delayed time of conjunctival hyperplasia. Combined use of mitomycin C seemed to be recommended.
The cutaneous connective tissue from a case of canine dermatosparaxis was examined morphologically. The collagen fibrils were 30 to 200 nm in diameter, showing random running and abnormal morphology and forming no bundles. Histochemically, reactivity of extracellular matrix to alucian blue pH1.0 was different from those of non-diseased cases, suggesting that dermatosparaxis might be due to changes of the extracellular matrix.
The viable numbers of Clostridium perfringens showed decrease of less than 1 log in chicken intestinal contents sampled as early as possible with the two end-legations and stored at 4°C for 2 days. In case of bovine feces kept at 4°C for 7 days in a plastic bag and Cary-Blair transport medium, the viable numbers of the organisms showed decrease of 1.5 log and less than 1 log, respectively.