Granulosa-cell tumor was found in a Holstein cow showing abnormal parturition. On 8 days prior to delivery, swelling, hyperemia and looseness of the vulva were remarkable. Vaginal examination revealed fully opened cervical canal and bloody mucus discharge, while enlarged udder or labour pains were not observed. No labor pains appeared for 8 days, so that she underwent artificial delivery. Her calf was apparently normal, but the placenta was retained. The cow secreted a small amount of brown mucous milk for a week. Three days after parturition, the enlarged left ovary was detected by rectal palpation and surgically removed 14 days after parturition. The ovary had many follicles with follicular fluid showing on estradiol 17β(E2) level of 1770 pg/ml. The uterus was very frail and not involved. After the ovarian extraction the milk production was increased and the E2 level was decreased to 5pg/ml or less though it was 36 pg/ml or more before extraction. The first estrus was observed 68 days after parturition.
In July 1993, a 3-week-old milk calf suddenly presented posterior trunk paralysis and at about one month of age disused for poor progenostic. Degeneration of nervus radicis spinalis and nonsuppurative encephalomyelitis were mainly located at substantia grisea medullae spinalis. Neospora protozoa was immuno-stained in the lesions using enzyme-labelled anti-Neospora caninum serum.
Two Anglo-Arab racing horses showed a recurrence of spontaneous and profuse nasal hemorrhage at an interval of less than one month. In one case there was a few masses of necrotic granulationes in the paranesal mucosa. The other was diagnosed as mycotic guttural pouch hemorrhage with destruction of the internal carotid artery wall and replacement by chronic granulation tissue. Both cases were diagnosed as epistaxis due to Aspergillus sp. by histopathology and scanning electron microscopy.
Histopathology of the skin disease was performed on 46 canine and 16 feline cases which were difficult in clinical and etiological diagnosis. Histology was effective in 20 canine and 4 feline cases for specifying the diseases and therapies, and it was helpful for the clinical treatment in 20 canine and 9 feline cases, while the disease entities could not be specified. Histology was ineffective in 9 cases, where treatments based on histological diagnosis had no effects. Therapies based on both the clinical findings and histological diagnoses were effective in 51 of 62 cases (80%).
An acute death occurred in 414 of 500 Formosan squirrels, freely reared at Park Y in a suburban district of Kanagawa Prefecture, from the middle October to November, 1992 and again, 3 of 156 died in the middle of May, 1993, showing mild facial swelling and bleeding from the nares and oral cavity. From 8 of the 9 dead cases β-hemolytic streptococcal strains were isolated and identified as S. zooepidemicus from their biochemical properties, and they were serotyped as Group C of Lancefield. As determined from the minimum inhibitory concentrations to 9 kinds of drugs, the isolates were highly susceptible to ampicillin, benzeylpenicillin, erythromycin, chloramphenicol and ofloxacin.
Effect of milbemycin oxime (MO) by oral route was evaluated in dogs with spontaneous demodicosis and mange. Three dogs with demodicosis given MO at 0.5 to 1 mg/kg did not show any improvement of the dermal signs, and the number of demodex mites in skin scrapings of these dogs unchanged. Although the other 2 dogs with demodicosis treated with 5 mg/kg showed a significant improvement of the dermal signs with regrowth of hairs, while the mites were not eradicated. Three dogs with mange were treated with 1 mg/kg in the same manner. The numbers of scabies mites were decreased significantly or disappeared, and the dermal signs were improved clearly. A dog concurrently infected with Demodex canis and Sarcoptis scabiei was treated with 5 mg/kg. While the number of scabies mites decreased within a short period and became undetectable level, and the dermal signs were abbreviated, demodex mites persisted in the skin.
A 5-year-old, female, Yorkshire terrier with gingival bleeding was revealed to have pancytopenia and hypoplasia in the bone marrow. Treatment by antibiotics, predonisolone, protein anabolic hormone, testosterone, erythropoietin and blood transfusion did not respond and died 26 days later of progressed anemia and thrombocytopenic suggillation. Hemorrhagic spots were present in most organs and the sternal marrow had only a small number of lymphocytes and plasma cells.
The rapid quantitative method of ampicillin (ABPC) in serum treated with methanol was achieved by micellar liquid chromatography (MLC) using an ODS reversedphase column, mobile phase containing 30 mM sodium dodecyl sulfate (SDS) and UV at 222 nm. The recovery rate of ABPC in bovine serum was about 98% and the detection limit was 0.4 ng, and no interference peak was seen with bovine, equine, and swine serum. The remaining amounts of ABPC in serum was 79% and 63% after storing at-40°C for 3 and 7 days, respectively, while 56% and 43% after storing at 5°C for 3 and 7 days, respectively.