Occurrence, structure and chemical composition of uric acid stones were examined in 269 newborn piglets before 18 days of age, which died or were killed for poor prognosis from June 1994 to May 1995. The stones were detected in 45 of 269 piglets (16.7%) at autopsy, at higher rates in April (39%), May (31%) and November (40%), while at lower rates in July (3%), August (9%) and September (8%). The highest occurrence rate (35.3%) was at 4 days of age, and 27 of 147 (18.4%) females and 18 heads of 122 (14.8%) males were shown to have stones. Nine stone samples were chemically analyzed, revealing six samples of uric acid and the other three of potassium urate. Scanning electron microscopy showed calculi 5 to 100μm in diameter and needle-like crys-tals surrounded by several shell layers.
The relationship between the incidence of calf white scour and alcohol stability of cow's milk (Utrecht abnormality) was investigated in 16 pairs of Japanese black cows and their calves with white scour within six-weeks after birth as well as 5 pairs of healthy controls. Of 16 cows with diseased calves, 9 (56.3%)(Group A) were positive for alcohol test, while 7 (Group B) were negative. The mean concentrations of blood NEFA and beta-lipoprotein were higher and the milk fat rate was lower in cows with diseased calves than controls, and their calves showed hypoliposis. Also, the mean concentration of blood ammonium was higher and glucose and calcium in whey were lower in group A than B. And the mean TG values were higher in group A than B. Menbutone (5g/day) was orally administered for 2 days to 9 cows of group A. Resulting in their milk turned to negative in 3 days and calves recovered from scour. Whey glucose were increased after administration of menbutone. The results suggested that the white scour in calves and the Utrecht Abnormality of milk might be due to the negative energy balance in cows, which was improved by menbutone treating.
Effect of a probiotic additive was investigated in 76 administered and 131 control Holstein heifers in a rear-ing pasture. The experimental heifers were administered the powdered probiotics daily for two weeks before pasturage, and the paste probiotics on the first day of pasturage and two weeks later. Serum α1-acid glycopro-tein and sialic acid in the experimental heifers were significantly lower than in the controls at one month of pas-turage. Leukocyte values in the experimental heifers showed no variation, while decreased at two weeks of pas-turage in the controls. At two months of pasturage glucose was significantly lower in both groups than the pre-pasturage level. Serum non-esterified fatty acid was significantly lower in the experimental heifers at one and two months of pasturage. Daily weight gain between the first day and one month of pasturage was signifi-cantly greater in the experimental heifers than in the controls. Average body weight of the experimental heifers on the first day of pasturage was lower than the standard value of Japan, increasing to the standard by the completion of pasturage.
Thirteen 125-day-old pullets were inoculated intramuscularly with 4.8×108colony forming units of an “Actinobacillussalpingitidis”/avianPasteurellahaemolytica-like isolate. Most of the inoculated birds showed. no clinical signs. In one of 6 cases sacrificed at 13 days postinoculation, the pericardium, was found to be severely thickened and adhered to the epicardium, and ruptured egg yolks were observed in the abdominal cavity in 4 of 7 cases sacrificed at 44 days postinoculation. Histopathology revealed pericarditis and heterophil infiltration in Glisson's sheath of the liver and theca of ovarian follicle. The organism was recovered from the brain, lung, heart, liver, kidney, ovary and oviduct of some cases.
From May to December 1994 at 9 of hoggeries in Z city, Shizuoka Prefecture, approximately 2, 800 piglets showed diarrhea, edema of eyelids, nervous symptoms, 7-14 days after weaning and 1, 400 died. Of 11 killed and 3 dead piglets, 3 cases showed cerebrospinal angiopathy at autopsy. EnterotoximicEscherichiacoliO 141: H4, produced verotoxin, was isolated (more than 107CFU/g) from duodenum contents of 8 cases. By the environmental investigation of 16 hoggeries in the city, E. coliO 141 was detected from floors of 8 farmhouses with the outbreaks as well as 5 farmhouses without outbreaks.
Heavy infestation of cat flea, Ctenocephalides felis, with itching sense was found in 9 dairy rearing calves kept in a paddock with saw dust floor mat. Four calves showed mild to moderate anemia. After treatment for one month with organo-phosphoric insecticide or carbamates, infesting fleas were remarkably decreased and simultaneous recovery from anemia was observed. However, fleas were again found on severed other new calves 3 days after their introduction to that paddock, suggesting that treatment was insufficient.
The ventrodorsal thoracic radiographic images were digitally analyzed by computer into 255 gray levels, and a gray level histogram was obtained based on frequency of each gray level. Five parameters (minimum gray level, maximum gray level, maximum frequency gray level, center gray level, mean gray level) in each histogram had reproducibility when the survey area was over 5% of a whole lung field. When the whole lung field was devided into cranial, middle and caudal areas, there were significant differences in parameters of each area. Therefore this method will provide the objective evaluation of survey thoracic radiographs.
In a one-year and three-month-old female mongrel cat with syncope, which was cardiographically diagnosed as third degree A-V block, isoproterenol administration was effective improving general condition, and no syncope (Adams-Stokes syndrome) occurred for the duration of four months.
A 48-day-old male pug (1.1kg) showing “setting sun sign” with dome-shaped head, open fontanelle and bilateral divergent strabismus, was diagnosed as hydrocephalus, by radiography, ultrasonography and ventriculography. After application of ventriculo-peritoneal shunt (V-P shunt) resulting in signs of success, the dog died from undetermined causes 7.5 months after the operation.
The germinal cell masses, probably immature brood capsules of Echinococcus multilocularis were dectected within cysts formed in the liver of a slaughtered pig. They were either papular thickenings of the germinal layer 8 to 15μm thick or germinal cell accumulations 6×10μm to 11X24μm in diameter within the cysts. No central lumen nor inner cuticle layer was formed. The cysts had a thin cuticle layer 4 to 9μm thick and germinal layers reaching 23μm in thickeness and some of them showed degerative changes, whereas no calcareous corpuscles and protoscoleces were observed.