The prevalence of Akabane and Aino viruses in bovine cases of congenital abnormalities was investigated from 1986 through 1995 in Hyogo Prefecture. Antibody against Akabane virus was detected in 1986, 1987, 1990, 1991 and 1994 mainly in the southern area, where 14 cases were pathologically and serologicaly diagnosed as congenital arthrogryposis-hydranencephaly (AH) syndrome from November 1994 through June 1995. Antibody to Aino virus was detected every year from 1986 to 1995 except for 1994 and two epizootics of congenital deformities were observed only in the southern area. The second epizootic of congenital abnormalities occurred in 28 calves from October 1995 through April 1996, and many of affected calves showed pathological findings of AH syndorome, including cerebellar hypoplasia. The precolostrum sera from 5 calves with AH syndrome were positive for neutralizing antibodies to Aino virus, suggesting that the epizootics was due to Aino virus.
Sera from a free-range laying roultry farm aged 120 to 582 days were tested for antibodies to Mycoplasma synoviae (MS) by gel diffusion precipitation (GDP) using commercially available antigen for rapid agglutination (RA). For each group, A sedimentation line fusing with that produced between the antigen and anti-MS immune chicken serum, appeared with some test sera of all age groups. Both RA and GDP positive reactions were observed in 1 of 16 and 15 of 20 cases of pullets aged 120days and those aged 190 days respectively. Bothpositive results were seen also in 14 of 20 and 13 of 20 sera from hens aged 405 days, and those aged 582days respectively. 0 sera of 120-day-old pullets as well as 1 to 2 cases of other groups were negative in both RA and GDP tests. Fifty or 70% of sera showing false positive reactions in RA were positive in GDP except for those from the 120-day-old group. The results suggested that, while GDP is less sensitive for detection of antibodies at an early stage of MS infection, it might be sensitive enough at later stage of infection.
Thirteen of 25 Tokara ponies pastured at Nakanoshima island in Kagoshima Prefecture died trom J July to September, 1994. A large number of nematode worms and eggs were detected from the caecum or colon of dead cases and from feces of surviving cases, respectively. All the nematodes detected were small round worms of subfamily Cyathostominae, belonging to 3 genera and 9 species. The surveys of the numbers of eggs per gram feces (EPG), and infective larvae adhered to grasses and the local climate revealed that the numbers of EPG and infective larvae increased with the rise of temperature, while they seemed not to be related to the rainfall throughout the year.
In 5 canine cases of idiopathic thrombocytopenic purpura (ITP) with more than 200×103/μl platelets after steroid or other immunosuppressive therapy, clinical findings, hemostatic screening test and platelet aggregation to adenosine diphosphate (ADP) were evaluated. While all the 5 cases showed no abnormal findings in hemostatic screening test, subcutaneous bleeding, ecchymoses and delayed hemostasis were continuously recognized in 3 of 5 cases after blood sampling, and defects of platelet aggregation against to ADP were detected in 4 cases, suggesting that bleeding tendency might be due to platelet dysfunction in some canine cases of ITP.
An 11-month-old male cat with frequent vomiting and diarrhea showed the congested extremity of the penis, increased values of BUN, Cre, T-Bil, AST and ALT in blood chemistry and severe occult blood in urinalysis. By abdominal radiography and intravenous urography, the case was diagnosed as urethral rupture. Modified spatulated end-to-end anastomosis was applied to the rupture after laparotomy, resulted in improved symptoms and satisfactory prognosis.
Ureal antifreeze was tested for acute stimulative effects on the eye and nasal mucosa of dogs after topical administration. I mmediately after ocular administration of a 10%(w/v) solution of the commercial antifreeze containing 99.3% urea, 10 dogs (9 mongrels and 1 beagle, with ages ranging between 4-5 years), showed mild blinking, which disappeared within 40 sec. With a 30% solution moderate blinking was induced and disappeared within 150 sec. Corneal injury was not observed in any cases, while one animal showed mild epiphora and several exhibited some behavior for strange taste of the compound. No remarkable signs were induced by intranasal administration of either concentration. Clinical signs were not severer even after five administrations of 30% solution at 15 min-intervals.
Eight (9.9%) of 81 and 7 (6.7%) of 105 sera from sheltered dogs and cats, respectively, were shown to have antibodies to Coxiella burnetii Nine Mile II by indirect immunofluorescence (IF). All the 8 sera from dogs and cats reared by infected human patients were positive for the antibody. C. burnetii was isolated from all the antibody-positive sera of dogs and cats reared by human patients as well as from 2 of 7 antibody-positive sera or spleens of sheltered cats by inoculation into cyclophosphamide-treated mice.