Bovine viral diarrhea virus (BVDV) 2 infection in cattle, swines, sheep, goat and deers was serologically surveyed in Ishikawa Prefecture. Only 1.1% of adult cattle had antibodies against BVDV 2, strain KZ-91, and retrospective serology with 1-year-old bovine sera collected since 1986, revealed that 1 of 70 cases of 1988, 2 of 51 cases of 1989 and 1 of 27 cases of 1994 had antibodies. Two of 10 BVDV isolates in 1990 were classified as BVDV 2, by restriction endonuclease analysis of reverse transcriptase-polymerase chain reaction (RT-PCR) using a primer set to match the 5' untranslated region sequence of pestivirus.
Thirty-one cases of bovine peracute mastitis due to gram negative bacteria were given oxytocin which was expected to eject the residual milk with bacteria, endotoxin and cytokyne, and then intrarterially treated with antibiotics and steroids for inflammation and cytokine effects. The patients were milked out immediately after the arterial injection, and some antibiotics and steroids were also infused intramammarily. Within 24 hr after the treament, clinical singns were improved and the lactation was restored almost to the normal levels. Six days later, they were all recovered without death nor culling.
The relation between the egg counts by filtration-floatation technique and Anoplocephara infection rate was examind in 102 dewormed and 20 dead horses. While only 28 of 102 (27.5%) horses were positive for the egg before deworming, the tapeworms were excreted from 60 (58.8%) horses after treatment. Of the 20 dead horses, only 3 (15%) had the eggs in the rectal feces, but 17 (85%) horses were shown to have the tapeworms at necropsy. On the other hand, the egg-positive and-negative cases were examined for the tapeworm infection, revealing that those infected with about 40 worms or less shed no eggs in the feces. From these results, it was concluded that the egg-positeve rate was too low to see the actual rate of equine Anoplocephara infection.
Plasma clearance of inulin for estimating glomerular filtration rate (GFR) was evaluated in 8 normal cats under a conscious condition. Areas under the clearance curve (AUC) were determined with 11 or 12 plasma samples by 2 common pharmacokinetic analyses, the two-compartment model (2-CM) and the computer generated nonlinear regression analysis (NLR). AUC was also determined by NLR in case where the number of samples was reduced to 6. There was no significant difference between AUCs determined by 2-CM and NLR with 6 samples and between the resulting AUCs and computed GFRs using inulin dosage. The regression line between AUCs determined by 2-CM and NLR with 6 samples and between computed GFRs using inulin dosage and the AUCs showed an excellent fitness based on p value and coefficient of determinations.
The effects of dexamethasone (DEX) were investigated in puppies inoculated with coronavirus (CCV), 5821 strain. In dogs inoculated intraduodenally or intravenously with CCV, and treated with DEX, virus was detected from the stomach, large intestines and mesenteric lymph nodes, while no virus was detectable from the stomach and large intestines without DEX. Dogs which were reared for longer time period after intraduodenal inoculation plus DEX, developed watery diarrhea, and virus was recovered from blood and feces. In cases inoculated intratracheally with CCV, the tonsil, lungs and mesenteric lymph nodes were positive for virus, irrespective of DEX administration. The results suggested that DEX might enhance the CCV infection in the digestive tract of dogs.
A female 13-year-old Shiba showed dysuria due to a peritoneal mass compressing the bladder. After the mass was removed, urination was readily induced by manual compression of the bladder, although urination was incontinent for 1 month. The animal was under a good condition for 7 months after treating with distigmine bromide. The mass was diagnosed as uterine leiomyosarcoma appearing probabley in relation to hystero-oophorectomy performed 3 years before.
A total of 5, 555 fishes (17 marine fish and 1 squid species) from Sagami and Suruga Bays were examined for Anisakis larvae in 1995 to 1996. The larvae were detected from 6.1% of Japanese anchovies, 33.7% of chub macherels, 2.0% of white-tipped macherel scads and 23.1% of frigate macherels collected from Sagami Bay, as well as from 3.5% of Japanese anchovies, 0.5% of Japanese pilchards, 21.0% of chub macherels and 33.3% of dolphinfishes collected from Suruga Bay. The average numbers of larvae per fish were 1.5 (1 to 3) and 6.5 (1 to 31) in Japanese anchovies and chub mackerels, respectively. Most larvae parasitized in the visceral organs and abdominal cavity, while a few in the muscles. Most larvae obtained were of Anisakis Type I larvae, Anisakis simplex, and a small number of Anisakis Type II and IV, Contracaecum Type B and D and Spirurid larvae were detecfed.
Twenty carcases of broiler chickens 51 days of age from a flock of 2, 542 birds were diagnosed as cholangiohepatitis at processing meat inspection. All affected cases showed enlargement and discoloration of liver with apparent acinar pattern. Enlarged gall bladder and extrahepatic bile ducts contained yellow inspissated material. Histopathologically, bile ductules were extensively proliferated with fibrosis forming interlobular bridges between triads. The portal bile ducts were destroyed and obstructed by multiple granulomas due to bacterial infection and the bile outflow. Infiltration of lymphocytes and plasma cells with germinal centers was also observed around granulomas. In these lesions many Gram-positive bacilli were observed and they were positive for Clostridium perfringens antigen by indirect immunofluorescence. C. perfringens was isolated and identified from affected livers.