The number of Cryptosporidium muris oocysts were monitored during compost making from bovine feces. After mixing bovine feces (260kg) with rice hull to adjust the moisture rate to 65%, the mixture containing 2.26×105 oocysts/g was piled up. Three days after the setting up, temperature was elevated to 73°C and the oocysts decreased in number to 0.1×105/g on day 8. Later temperature lowered and a few oocysts were detectable. After refermentation by adding rice bran and water, no oocysts were detectable on day 44.
The skin of a cow showing cutaneous fragility and delayed wound healing was revealed to have rarefaction of collagen fibers and increased amount of dermal ground substance by electron microscopy. Biochemical analysis of the skin exhibited dermatan sulfate deficiency without accumulation of procollagen, suggesting that the systemic skin fragility and extensibility were induced by deficiency in dermatan sulfate but not by lack of procollagen aminopeptidase.
Serum components were examined in 112 Japanese Black calves aged 1 to 73 days. No significant correlation was observed between ages and serum free fat acids, triglyceride or glucose levels. However, significant linear correlations were seen between ages and concentrations of serum total protein, β-globulin and γ-globulin. The levels of albumin, total cholesterol and phospholipid increased till 20 to 30 days of age, whereas those of α-globulin, sialic acid and BUN decreased till 10 to 30 days after birth.
SPF kittens were subcutaneously or orally inoculated with the 2a antigenic type canine parvoviruses (CPV) including strain FPV-314 isolated from a case of feline panleukopenia. Those inoculated with CPVs did not manifest clinical symptom except slight leukopenia, while CPV was recovered from feces and some organs and neutralizing antibody was detected. Sera of cats treated by a feline panluekopenia virus (FPLV) vaccine neutralized CPVs in vitro, suggesting that the FPLV vaccine might be effective in preventing CPV infection in cats.
In cats with gingivostomatitis CD8+ T-lymphocytes (993/, μl) remarkably increased in number (p<0.01), and the CD4+ /CD8+ ratio was lower (p<0.01) as compared to healthy animals, while correlation was not seen between the CD4+/CD8+ ratio and the degree of gingivostomatitis. The number of CD4+ T-lymphocytes in the peripheral blood remained unchanged.
An 8-year-old spayed female mixed-breed dog showed anorexia and severe anemia and the condition was improved after blood transfusion. On day 35 of illness, however, the patient showing anorexia and vomitting had pain in the hindquarter, severe anemia and elevated levels of serum protein and calcium. Two monoclonal peaks of gamma-globulin fraction were shown by electrophoresis, and the M-component was immunoelectrophoretically confirmed to be IgA. Splenomegaly and “punch-out” osteolysis were detected by radiography. Peroxidase-negative and IgA-positive mononuclear cells were predominant on bone marrow biopsy and the case was diagnosed as multiple IgA myeloma (stage III), and melpharan and predonisolone treating was started. On day 84 of illness the patient died of sudden dispnea and vomitting.
High-performance liquid chromatography (HPLC) was applied to determination of residual cephalosporins in beef meat. Five drugs, cefazolin, cefalonium, cefuroxime, cephapirin and ceftiofur, were added to meat samples (0.5μg (p)/g), which were extracted with 0.3% metaphosphoric acid-methanol (7: 3), and the extract was cleaned up on an Oasis HLB cartridge. The HPLC separation was carried out on an Inertsil ODS-3V column (4.6mm ID-250mm) with a mobile phase of 25mmol/l phosphate buffer (pH 2.8)-acetonitrile (75: 25) at a flow-rate of 1ml/min. The extracts were analyzed using a UV detector with time programming. The recoveries at levels of (0.5μg (p)/g) were 78.3 to 91.6%, and the detection limit of each drug was 0.05μg (p)/g.
By homogenization in stomacher bags, the isolation rates of Campylobacter jejuni from chicken skin and meat samples stored at 4°C t for 3 days were improved. The isolation rates were reduced after longer time cultivation of the homogenates in an enrichment medium.