A tumor mass 8 cm in diameter, was noticed on the left lumbar area of a 5-year-old dairy cow and enlarged to 16 cm in diameter 5 months later. The cow calved one month later, but was euthanatized at 2 months postpartum, because of deteriorating condition with decreased milk production. Grossly, the epidermis overlying the tumor was hyperkeratotic and partially ulcerated. The tumor affected the dermis, subcutis and muscles with necrotic or hemorrhagic foci at the center, extending to the peritoneum. The left subiliac, supramammary, medial iliac and lumbar aortic lymph nodes were enlarged. Microscopy revealed that the tumor tissue consisted of dense proliferation of fibroblastoid and histiocytoid cells, partly with foamy cells or multinucleated giant cells. Erythrophagia by histiocytoid cells was observed in hemorrhagic foci. Ultrastructurally, histiocytoid cells had abundant cytoplasmic organelles, as well as desmosome-like structures between adjoining cells.
The teat orifices of subclinical mastitis cases in dairy cattle were morphologically classified into four types: 1) smooth and well closed (type I), 2) smooth and with small ring (type II), 3) smooth and with large ring (type III and 4) hyperkeratotic (type IV), and the relationship between the teat orifice abnormalities and the occurrence of mastitis was investigated. Fourty-nine out of 150 quarters were shown to have subclinical mastitis based upon bacterial and cell counts, and the positivity rates were 8.3%, 23.1%, 34.8% and 40.8% in types I, II, III and IV, respectively. Histopathology revealed hydropic degeneration in the streak canal of all samples, and cutaneous hyperkeratosis reached the streak canal with remarkable epithelial ablation of the rosette in type IV teats. These results suggested a close correlation between teat orifice abnormalities and the occurrence of subclinical mastitis.
During a period of September and October 1997, 44 cases of abnormal delivery, mainly abortion and stillbirth, occurred in Fukuoka Prefecture. Neutralizing antibody to Ibaraki virus (IBAV) was detected from 81.8% of the affected maternal cows, and 14 virus strains were isolated from maternal blood samples, placentas and fetal brains using HmLu-1 cell culture. During the same period 7 cases with dysphagia were shown to have neutralizing antibody to IBAV, and 2 virus strains were isolated from blood cells and identified physicochemically and serologically as IBAV. Blood samples collected from 100 sentinel calves spending the first summer season at 50 farms in 1997 showed seroconversion; 5 cases in September, 38 cases in October and 13 cases in November. These findings suggested that IBAV might have been concerned in the multiple cases of abortion and stillbirth.
Transscleral cyclophotocoagulation using diode laser was performed on 5 eyes of 4 dogs with glaucoma showing intraocular pressures (TOP) of 43 to 58 mmHg. The IOPs of 2 eyes were increased temporarily on Days 5 and 6, respectively, and one of these showed IOPs of 46 to 48 mmHg on Day 36 again and received another diode laser cyclophotocoagulation. All the eyes showed IOPs of less than 27 mmHg during 76 to 168 days postoperation. On Days 2 to 7 postoperation eye discharge, anterior chamber flare, corneal opasity, scleral congestion, conjunctival hyperemia, chemosis, photophobia and miosis were observed, but these complications were resolved by Day 14.
In 1996 and 1997 three dead and two living Iriomote cats (Felis iriomotensis) were brought to the Wildlife Conservation Center of the Environmental Agency, Iriomote Island, Okinawa Prefecture. Two of the three dead cases were adult and due to traffic accidents, while the other juvenile was suggested to be fatally bitten by some animal. One of the two living cases was seriously injured by traffic accident and successfully recovered. Another living case, supposedly a suckling of a dead case mentioned above, grew up well and was released into the wild life, but 3 months later it was found dead by unknown cause.
Seven canine and 1 feline cases with postsurgical wound infection were suspected of multi-resistant Staphylococcus aureus (MRSA) infection and the isolates were examined for resistance to methicillin. Two of the 8 isolates were methicillin-resistant MRSA, whereas others were non-methicillin-resistant S. aureus (1 case), S. intermedius (2 cases) and S. delphini (2 cases). The remaining one was unable to identify. According to rapid amplified polymorphism DNA method, 2 strains were suggested to be of intramural infection.
The survey of zoonotic bacteria has been carried out on feces of iguanas, prairie dogs and chameleons etc. in October and November in 1996 and 1997. These animals have been kept at the pet-shops or the ordinary residences in Tama area of Tokyo. The results show thatSalmonella3 (2.1%), Staphylococcus aureus 2 (1.4%) andChlamydia8 (5.7%) were detected from 140 animals in 1996 andSalmonella5 (5.0%), Listeria monocytogenes1 (1.0%) andChlamydia4 (4.0%) from 101 animals in 1997.
Four Cases of Holstein-Friesian cows, 4 2/3 to 7 years old, were slaughtered due to unfavorable clinical diagnoses such as lumbar paralysis, pelvic trauma, and chronic necrotizing pododermatitis, and were diagnosed as generalized lipofuscinosis. In all cases, most of the skeletal, tongue, masseter and diaphragm muscles were grossly tinged with dark brown, and the cardiac muscle, liver, kidney, adrenal cortex, muscular layer of the small intestines and cerebral cortex were also affected with brown discoloration. Microscopy revealed a generalized intracytoplasmic deposition of fine or coarse granules, tinctorially identical to lipofuscin, which were PAS-positive, sudanophilic, acid fast, and yellowish-orange autofluorescent. The cardiac muscle fibers (1 case), diaphragm (3 cases), and intestines (all cases) had intracytoplasmic eosinophilic granules or hyaline droplets of various sizes up to 14μm in diameter. The diaphragmatic muscle fibers (3 cases) had central autophagic vacuoles. No glial reaction was seen around nerve cells containing lipofuscin pigments.