Journal of the Japan Veterinary Medical Association
Online ISSN : 2186-0211
Print ISSN : 0446-6454
ISSN-L : 0446-6454
Volume 53, Issue 3
Displaying 1-9 of 9 articles from this issue
  • Yataro KOKUBO, Akira CHAZONO
    2000 Volume 53 Issue 3 Pages 121-129
    Published: March 20, 2000
    Released on J-STAGE: June 17, 2011
    JOURNAL FREE ACCESS
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  • Yoshitaka MATSUI, Tsunao HIRAI, Sadao ONOE, Naohito KUSAKARI, Satoshi ...
    2000 Volume 53 Issue 3 Pages 131-135
    Published: March 20, 2000
    Released on J-STAGE: June 17, 2011
    JOURNAL FREE ACCESS
    Twelve postpartum, nursing Angus and Hereford beef dams were divided in to2groups. In the experimental groups, feed enriched with Selenium (Se) was given to 6 dams (Se: 0.525mg/100 kg of body weight/day) for 11 weeks. The second, or control, group consisted of 6 beef dams species- and aged-matched with the dams in the experimental group and kept under the same conditions except for Sesupplementation. Suckling calves were examined for serum-Se concentration, peripheral neutrophil function, andlymphocyte blastogenesis. After 3 weeks, serum-Se concentration was significantly higher in calves ofdamsreceiving Se supplements than in those of control dams. Assays using whole blood and isolated granulocytesshowed that, at 5 weeks, in the experimental group, it took significantly less time to reach peakchemi-luminescence (CL) than it did in control calves. Furthermore, after 5 weeks, response of lymphocyte-stimulatedconcanavalin A was significantly higher in experimental animals than that in control animals. These resultssuggest that feeding dams Seenriched yeast enhances the neutrophil and lymphocyte functions of sucklingcalves, to which Se is transferred through milk.
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  • Shigeru SATO, Toshihiro ICHIJO, Toshiyuki SUZUKI, Hideya ONO
    2000 Volume 53 Issue 3 Pages 136-139
    Published: March 20, 2000
    Released on J-STAGE: June 17, 2011
    JOURNAL FREE ACCESS
    Serum concentrations of total and free biotin and biotinidase activity were investigated in diary cows with, such hoof disorders as sole ulcer, heel erosion and foot rot (interdigital necrobacillosis). The agar-plate method of biotin bioassay using Lactobacillus plantarumATCC 8014 and the colorimetric biotinidase-assay method were employed. Total-biotin serum concentrations were significantly lower in cows with sole ulcer (P< 0.05) and heel erosion (P< 0.01) than in normal cows. Free-biotin serum concentrations in cows with hoof disorders were similar to those in normal cows. No significant difference was observed in serum biotinidase activity between cows with hoof disorders and normal cows. Positive correlations were observed, however, between biotinidase activity and concentrations of total and of free biotin. It may be that biotin ingestion from feed and biotin synthesis by rumen bacteria may decrease below levels in normal cows in dairy cows with, such hoof disorders as sole ulcer and heel erosion.
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  • Kenichi ITO, Kazuharu KAWASHIMA, Miyuki SAKURAI, Shigeru TAKAMIZAWA, T ...
    2000 Volume 53 Issue 3 Pages 140-143
    Published: March 20, 2000
    Released on J-STAGE: June 17, 2011
    JOURNAL FREE ACCESS
    To investigate the role of apoptosis in the formation of necrotizing hepatitis in cases of the rabbit hemorrhagic disease (RHD), we examined 3 rabbits killed by a natural outbreak of the disease in Shizuoka Prefecture in 1995 and 5 rabbits experimentally inoculated with the RHD virus (Shizuoka strain). All of the inoculated rabbits either died or were killed at from 26.5 to 35 hours after infection. Histological investigation showed severe necrotizing hepatitis in the periportal areas of the liver and fibrinous thrombosis in the lungs and kidneys. Acidophilic bodies, condensed and marginated nuclei, and karyorrhexis of the hepatocytes were observed in and around the necrotic lesions. TUNEL-positive hepatocytes were scattered mainly at lobe peripheries. Electron-microscopic investigation revealed apoptotic cells with condensation of the marginal nuclear chromatin. These findings suggest that apoptosis of the hepatocytes contributes to the pathogenesis of hepatic lesions in RHD.
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  • Tadashi TUBOKAWA, Shuji HONDA
    2000 Volume 53 Issue 3 Pages 144-147
    Published: March 20, 2000
    Released on J-STAGE: June 17, 2011
    JOURNAL FREE ACCESS
    Beginning in June 1997, in a hoggery under our jurisdiction, on a farm in Toyama Prefecture, we began occasionally finding major clinical signs of poor growth in some young weaned pigs. This condition was sometimes accompanied by pale skin, loss of vigor, and diarrhea. Histopathological examinations revealed lymphadenopathy, hypolymphemia, and basophilic inclusion bodies in reticuloendothelial cells in the affected pigs. Electron microscopy detected circovirus-like particles in the inclusion bodies. These clinical and pathological findings resemble those observed in cases of post weaning multisystemic wasting syndrome (PMWS). The DNA sequence of PCR products from the pigs' lymph node emulsions showed a homology higher than 96.9% to the circovirus suspected of being the PMWS causal factor (pmws-PCV). These results suggest that the pmws-PCV is involved in the disease of the young pigs.
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  • Nobuya HISHIYAMA, Junshiro HIROMA, Kazunari HIGA, Hiroshi FUJISE
    2000 Volume 53 Issue 3 Pages 149-153
    Published: March 20, 2000
    Released on J-STAGE: June 17, 2011
    JOURNAL FREE ACCESS
    Such clinical indices as acid-base disturbance and electrolytes were examined in aclinical case of hemolytic anemia presumed to be suffering from onion poisoning and an experimental case towhich onion was administered in high K and high glutathione (HK/HG) dogs. In the clinical case, on theday of admission (day 1), a drop in PCV to 15.9% and in Hb of 5.0 g/dl indicated severe anemia. On that sameday, blood HCO3-decreased because of metabolic acidosis. Low PCO2, however, indicated that respiration wascompensating for the acidosis. On day 2, HCO3-remained low, and pH dropped to 7.25, indicating acidemia.But on day 3, HCO3-began to increase, and blood pH recovered to 7.38. By day 12, the anemic condition haddisappeared. Very similar results obtained in the experimental case strongly suggest that the clinical casewas caused by onion poisoning. In neither case did hyperkalemia resulting from hemolysis of HK/HG red cells occur.
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  • Hideki KAYANUMA, Tsunenori SUGANUMA, Toshifumi WATANABE, Yasuo NOMURA, ...
    2000 Volume 53 Issue 3 Pages 154-156
    Published: March 20, 2000
    Released on J-STAGE: June 17, 2011
    JOURNAL FREE ACCESS
    Myelographic examination of an 8-month-old female golden retriever withprogressive bilateral hind-limb palsy indicated the presence of an intradural, extramedullary mass at the level of L1. Laminectomy followed by durotomy was performed to remove the mass. Histopathological examination of theexcised tissue showed epithelial nephroblastoma characterized by urinoferous tubule-like structures andglomerulus-like structures. The dog recovered and, 13 months after surgery, showed neither neurologicalproblems nor metastasis.
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  • Tsukasa OGANE, Yu UCHIDA, Takatoshi KIKUCHI, Satoshi SAITO, Susumu OFU ...
    2000 Volume 53 Issue 3 Pages 159-162
    Published: March 20, 2000
    Released on J-STAGE: June 17, 2011
    JOURNAL FREE ACCESS
    The former bovine slaughtering process involved skinning the breast and forelegafter the gullet was tied. We improved the process and minimized the stripping area by using a standing-typeskin stripper immediately before whole skinning. In addition, in 1998, we replaced the at-the-deskhygienic lectures we had held until 1997 for meat-plant personnel with practical discussion and trainingsessions conducted while actual slaughtering was taking place. As a result of these measures, total values ofcarcasses increased markedly while total numbers of viable bacteria and ofEscherichiacoli in carcassessignificantly decreascd.
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  • [in Japanese]
    2000 Volume 53 Issue 3 Pages 169-170
    Published: March 20, 2000
    Released on J-STAGE: June 17, 2011
    JOURNAL FREE ACCESS
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