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Volume 53, Issue 4
Displaying 1-12 of 12 articles from this issue
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Focusing on Avain Viral Vector-based VaccinesKenji TSUKAMOTO2000 Volume 53 Issue 4 Pages 189-194
Published: April 20, 2000
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSDownload PDF (1203K) -
Noritoshi KAWATE, Makoto TSUJI, Hiromichi TAMADA, Toshio INABA, Tsutom ...2000 Volume 53 Issue 4 Pages 195-199
Published: April 20, 2000
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSAfter an injection of 100μg of GnRH analogue, a functional corpus luteum was formed in a Holstein cow diagnosed as follicular cystic. The follicular cyst recurred, however, after the regression of the corpus luteum. To examine estrogen sensitivity in the hypothalamus of the cystic cow, 1 mg of estradiol-17β(E2) was injected, and LH concentration was determined. No LH surge was detectable in the cystic cow, although peak LH surge occurred 24 hours after E2 injection in 3 cows with normal estrous cycles. These results suggest that reduced sensitivity to estrogen in the hypothalamus is one cause of recurrent follicular cyst in this particular cow.View full abstractDownload PDF (1579K) -
Kazuyuki SUZUKI, Tadaharu AJITO, Yoshifumi MURAKAMI, Hideyuki ANZAI, S ...2000 Volume 53 Issue 4 Pages 200-204
Published: April 20, 2000
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSDoses of 2.0μg/kg of Escherichia coli serotype 0111: B55 endotoxin dissolved in 1, 000ml of pyrogen-free isotonic saline solution were administered over 5-minute periods via the jugular vein to 4 heifers. After initiation of endotoxin infusion, the heifers were monitored until 240 minutes had passed. After decreasing progressively, at t=30min, the relative plasma volume had dropped to 67.4±6.0% and partial oxygen pressure to 56.4±2.4mmHg. Thereafter, they remained constantly at low levels. The arterial-alveolar O2 gradient increased progressively to 44.6±3.2mmHg by t=30min. These results suggest that this model meets experimental requirements for examination of the bovine respiratory-disease complex.View full abstractDownload PDF (723K) -
Kouji OHWADA, Takayuki MURAKAMI2000 Volume 53 Issue 4 Pages 205-209
Published: April 20, 2000
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSWe investigated 469 cases of congenital heart diseases in malformed bovine hearts collected mainly in Miyazaki Prefecture from 1979 to 1997. A total of 893 diseases, subdivided into 53 types, were represented. Most frequently encountered was ventricular septal defect (198 cases; 42.2%).This was followed by atrial septal defect (148 cases; 31.6%), double-outlet right ventricle (66 cases; 14.1%), aortic stenosis (53 cases; 11.3%), double cranial venae cavae (50 cases; 10.7%), patent ductus arteriosus (45 cases; 9.6%), anomalos caudal vena cava with azygous continuation (42 cases; 9.0%), the tetralogy of Fallot (32 cases; 6.8%), hypoplastic left ventricle syndrome (25 cases; 6.8%), complete transposition of the great arteries (24 cases; 5.1%), and total anomalous pulmonary venous connection (21 cases; 4.5%). Simple cardiac defects were detected in 251 cases (53.5%) and complex cardiac in 218 cases (46.5%). This study demonstrates that in cattle as well as in human beings and cats, ventricular septal defect is encountered most frequently.View full abstractDownload PDF (1465K) -
Kouji OHWADA, Takayuki MURAKAMI2000 Volume 53 Issue 4 Pages 210-214
Published: April 20, 2000
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSMorphological observations of 4, 301 bovine necropsied cases collected in Miyazaki Prefecture between 1979 and 1997 at the Miyazaki University showed an incidence of congenital heart disease (CHD) of 8.7%(373/4, 301) among the necropsied cases and 13.2%(373/2, 826) among malformed cases. These values are higher than those in previous reports on both animals and human beings. Anal atresia, cleft palate and anomalies of the eye, the spleen and genito-urinary system, and the sternum and costae were the most frequent CHD-associated extracardiac abnormalities.View full abstractDownload PDF (1576K) -
Takashi ASANO, Yoshisato YOSHIKAWA, Makiko MURAKAMI, Shinichi KUMAGAI, ...2000 Volume 53 Issue 4 Pages 215-217
Published: April 20, 2000
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSEpidemiological investigations were carried out on coumarin poisoning of wildbirds in an apple farm in the city of Morioka, Japan, in March 1999. Numerous apples polluted by a bluechemical compound were found scattered on the ground together with about 50 bird carcasses. Necropsy showedhemorrhagic spots on the skin, skeletal muscles, and frontal bones and thoracic cavities filled withhypo-coagulative clots. Hyperemia of the conjunctiva and food-caused dilation and atony of the upper digestive tractwere recorded. Histopathological studies demonstrated hemorrhagic changes in the lungs, the glandularstomach, and the choroid. Systemic congestion was observed in all of the 8 cases examined. High-performance liquidchromatography performed on an affected apple, bird livers, kidneys, and gastric contents showed from 0.2to 10.9 ppm of coumatetralyl in each sample. This is the first report in Japan of coumarin poisoning of wild birds with sufficient concentration for its toxicity.View full abstractDownload PDF (1208K) -
Yoshiharu OKAMOTO, Kota SATO, Yoshiaki HIKASA, Takehito MORITA, Akinor ...2000 Volume 53 Issue 4 Pages 218-220
Published: April 20, 2000
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSA 6-year-old Golden retriever suffering from vomiting and an enlarged abdomenshowed hematologic and biochemical abnormalities, including marked leukocytosis, azotemia and highalanineaminotransferase (ALT). A radiographic survey of the abdomen led to suspicion of ascite accumulation. Although these symptoms disappeared after initial treatment with antiseptic and diuretic agents, the sameclinical signs recurred after two weeks. When an exploratory laparotomy was performed, the left hepatic lobe was revealed to be enlarged and located at the position of the spleen. Examination of the hilar region revealed counterclockwise torsion of the lobe. The affected lobe was then excised. Histopathological examination showed severe degeneration and necrosis of the hepatocytes, and fibrosis in the lobe.View full abstractDownload PDF (1431K) -
Yoshihiro OHMICHI, Atsushi HIRAKAWA, Yoshiaki TAKAHASHI, Hinako SHIBAY ...2000 Volume 53 Issue 4 Pages 221-224
Published: April 20, 2000
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESShypercalcemia, and radiography revealed an abdominal mass. Experimentallaparotomy showed a large, solid mass originating from the liver. Because the mass was big and tended to bleedwhen manipulated, we abandoned complete excision and collected samples for histophathology. The patientdied 2 days after the operation. The mass was diagnosed as hepatoblastoma. Elevated parathyroidhormone-related protein (PTH-rP) and serum α-feto protein levels suggested that the PTH-rP secreted by hepatoblastomacells led to hypercalcemia in the patient.View full abstractDownload PDF (2554K) -
Seiji ASAMI, Kazumi HATSUGAI, Kazuyuki OSAWA, Yoshio NAKABAYASHI, Taka ...2000 Volume 53 Issue 4 Pages 225-231
Published: April 20, 2000
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSA gas chromatographic/mass spectrometric method (GC/MS) using selected ion monitoring (SIM) was developed for the determination of oxolinic acid, ofloxacin, enrofloxacin, and danafloxacin in porcine meat and kidneys. Quinolone antibacterials were extracted with 0.3% metaphosphoric acid-acetonitrile (6: 4) followed by a Sep-Pak PS-2 cleanup procedure. After evaporation, the residue was dissolved in methanol and reduced with sodium tetrahydroborate treated with 0.1N HC1. Quinolone derivatives were extracted by a Sep-Pak PS-2 from the reaction mixture and analyzed by GC/MS (SIM). Average recoveries of the drugs from tissues were in the ranges of 80.8-90.5% in meat and 82.3-88.6% in kidneys. Detection limits were 0.01μg/g for oxolinic acid, ofloxacin, and enrofloxacin and 0.02μg/g for danofloxacin.View full abstractDownload PDF (914K) -
Akihiko UCHIDA, Kikue UCHIDA, Yoshihiko MURATA2000 Volume 53 Issue 4 Pages 232-234
Published: April 20, 2000
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSThe authors conducted morphological and epidemiological studies of endoparasites in Japanese mammalians. Metagonimus yokokawai and Spirometra erinaceieuropaei were obtained from the smallintestines of masked plam civets, Paguma larvata captured near Sagamihara, Kanagawa Prefecture and Kannami, Tagatagun, Shizuoka Prefecture, Japan. This report states that Paguma larvata is a new final host for Metagonimus yokogawai and Spirometra erinaceieuropaei.View full abstractDownload PDF (1280K) -
2000 Volume 53 Issue 4 Pages 239
Published: April 20, 2000
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSDownload PDF (104K) -
[in Japanese]2000 Volume 53 Issue 4 Pages 241-242
Published: April 20, 2000
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSDownload PDF (1140K)
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