Optimum enrichment broth, selective isolation media, and their culture conditions were examined for the purposes of isolating
Arcobacter butzleri and
Arcobacter cryaerophilus. Optimum enrichment-culture conditions for the
Arcobacter species were obtained by using
Arcobacter Selective Broth at 25°C for 2 to 3 days under aerobic conditions. An aerobic culture using modified CIN agar and either Skirrow blood agar or Preston blood agar at 25°C for 2 to 3 days was suitable for isolating both Arcobacter species. Results of tests for catalase, nitrate reduction, 0.04% TTC resistance, and nalidixic-acid resistance and polymerase chain reactions differentiated
A. butzleri, A. cryaerophilus, and thermophilic Campylobacters. In addition, the prevalence of
Arcobacter species was investigated in 80 samples of retail chicken meat and 20 chicken livers. Twenty percent of the 180 samples were contaminated with
Arcobacter species.
A. butzleri was isolated from 11 samples, A.cryaerophilus from 6 samples, and both
A. butzleri and
A. cryaerophilus from 3 samples.
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