Pathological and etiological field examinations were made to six commercial layer hens with giant facial masses from three farms. The masses in the field cases were diagnosed histologically as myxoma or fibrosarcoma (myxomatous type). In immunohistochemical terms, cellsfrom each tumor were positive for the groupspecific ALV antigen. Specific amplifications of subgroup A ALV genes obtained by means of the PCR method suggest that subgroup A ALV could play a part in tumor formation. Experimental studies were performed on two groups:(1) chickens inoculated with homogenates of tumor tissues from field cases and (2) verticallyinfected chickens hatched from eggs of chickens inoculated with tumor-tissue homogenates. Vertically infected chickens reproduced myxomas similar to those found in field cases, whereas inoculated experimental chickens did not.
A four-year-old Holstein-Friesian cow, in which rectal palpation demonstrated a large massat the left kidney, was examined pathologically. Macroscopically, the left kidney was markedly enlarged, hard, and pale in color. The lobular structure was preserved. On its cut surface, hard whitish tissue had displaced the renal parenchyma. Numerous hard, whitish masses (1 to 10cm in diameter) were detected between the left renal lymph node and the left iliac lymph node. In histopathological terms, irregular tubules lined with atypical neoplastic epithelial cells were diffused through the left kidney, remarkably proliferated connective tissue, replacing the renal parenchyma. Similar tumor tissues were observed in other hard, whitish masses. The tumor cells stained positive for the anti-uromodulin antibody. Ultrastructural examination revealed the tumorous cells to have microvilli and desmosome structures. On the basis of these findings, the tumor was diagnosed as renal cell carcinoma with marked connective-tissue proliferation.
A total of 460 household cats aged from one month to 16 years in the Hachinohe area, Aomori prefecture, Japan, were subject to a fecal examination for parasites using a formalin-ethyl acetate sedimentation technique. Of the cats, 22.4% were positive for intestinal parasites. The parasites detected and their rates were: Toxocara cati 13.9%, Ancylostoma tubaeforme 2.4%, Strongyloides planiceps 0.2%, Spirometra erinaceieuropaei3.0%, Taenia sp. 2.2%, Isospora sp. 2.0% and Giardia intestinalis 3.3%. The prevalence of T. cati in kittens of one to six months old was higher than that in older cats, while the prevalence of A. tubaeforme, S. erinaceieuropaei and Taenia sp. in cats of two to five years old was higher than that in cats of other ages. The prevalence most of the parasites detected in the present study was higher in cats kept outdoors rather than indoors.
The preventive effects of Bacillus subtilis DB9011 (DB9011) on the immunosuppression induced by anesthesia and surgery were investigated in dogs. DB9011 was administered before andafter operation, and a chemiluminescence (CL) assay of the canine neutrophils, macrophagesand peripheral blood lymphocytes (PBL) was performed. In dogs receiving DB9011, CL activity of macrophages and PBL showed a significant increase from one day post-operation compared to that in dogs without the administration of DB9011. Especially, PBL activity was maintained with a more significant increase. These results suggest that daily administration of DB9011 before and after operation may prevent immunosuppression at the earlier phase of the postsurgical period.
dog diagnosed as insulinoma with concurrent peripheral polyneuropathy was treated with surgical resection and postoperative streptozotocin (STZ) therapy. Beginning on the 62nd postoperative day, intravenous administration of STZ (500mg/m2) was performed every three weeks. The dog's blood glucose level returned to the normal range and neurological signs improved after the administration of two doses of STZ. At the third administration of STZ, recurrent hypoglycemia was identified. The dog's condition did not improve and a seizure followed. The dog was euthanatized due to the development of the hypoglycemic seizure on the 127th postoperative day. Normoglycemia had been maintained without severe side effects, such as renal dysfunction, f or two months after the operation. We therefore suggest that STZ therapy is useful in the medical management of canine insulinoma.
We investigated the prevalence ofBartonellaspp. in 346 cats in a compound (sheltered cats, including 88 newborn cats), 84 pet cats in animal clinics and 72 dogs in a compound (sheltered dogs) in Gunma Prefecture. The genomic diversity ofB. henselae isolateswere also examined by pulsed-field gel electrophoresis (PFGE) with Not I and Sma I restriction enzymes.Bartonellaspp. were isolated from 4.3%(11/258) of sheltered cats excluding newborn cats and 3.6%(3/84) of pet cats, but not from the sheltered newborn catsand dogs. Of these positive cats, B. henselae was detected in 11 sheltered and three pet cats, B. clarridgeaewas detected in only oneB. henselae-positivepet cat. All B. henselae isolates (33 strains) belonged to the 16S rRNA gene type I and showed genetically diverse genome patterns. Our data suggested that vertical transmission of Bartonellaspp. may not occur in cats. The cats with positive results in Gunma Prefecture have been co-infected with B. henselae type I with various genetic divergences.